Thermodynamics of reverse electron transfer across site 1: ATP/2e- is greater than one.

abstract：:Diphtheria toxin (DT) binds to a specific heparin-binding epidermal growth factor-like growth factor (HB-EGF) precursor that is expressed in DT-sensitive cells. DT binds to the cell-surface HB-EGF precursor with an apparent dissociation constant (KD) of approximately 1 x 10(-8) - 10(-9) M at 4 degreesC, a temperature ...

journal_title：Biochemical and biophysical research communications

authors： Brooke JS,Cha JH,Eidels L

更新日期：1998-07-20 00:00:00

abstract：:Monoclonal antibodies are valuable laboratory reagents and are increasingly being exploited as therapeutics to treat a range of diseases. Selecting new monoclonal antibodies that are validated to work in particular applications, despite the availability of several different techniques, can be resource intensive with u...

journal_title：Biochemical and biophysical research communications

authors： Staudt N,Müller-Sienerth N,Wright GJ

更新日期：2014-03-21 00:00:00

abstract：:Genotoxic agents of environmental or dietary origin may play a role in breast cancer initiation. The ability of extracts of human milk to cause mutations in S. typhimurium TA1538 and YG1019 and to induce micronuclei and DNA strand breaks in MCL-5 cells was investigated. Twenty samples from different donors were analys...

journal_title：Biochemical and biophysical research communications

authors： Martin FL,Cole KJ,Weaver G,Grover PL,Phillips DH

更新日期：1999-04-13 00:00:00

abstract：:The activation of vitamin D requires 25-hydroxylation in the liver and 1alpha-hydroxylation in the kidney. However, it remains unclear which enzyme is relevant to vitamin D 25-hydroxylation. Recently, human CYP2R1 has been reported to be a potential candidate for a hepatic vitamin D 25-hydroxylase. Thus, vitamin D met...

journal_title：Biochemical and biophysical research communications

authors： Shinkyo R,Sakaki T,Kamakura M,Ohta M,Inouye K

更新日期：2004-11-05 00:00:00

abstract：:Previously, we have investigated the potential for a pro-oxidant interaction of iron and ascorbate in vivo in iron and ascorbate cosupplementation or ascorbate supplementation studies. In this study, for the first time, the effects of iron supplementation on oxidative damage to DNA in healthy individuals with plasma a...

journal_title：Biochemical and biophysical research communications

authors： Proteggente AR,England TG,Rice-Evans CA,Halliwell B

更新日期：2001-10-19 00:00:00

abstract：:Specific receptors for murine TNF have been identified in homogenates of rodent brain. These receptors are saturable and bind TNF with sufficient affinity to ensure occupancy by cytokine elaborated during infection. 125I-mTNF was detected in four specific complexes of Mr 130,000, 90,000, 66,000 and 60,000 after affini...

journal_title：Biochemical and biophysical research communications

authors： Kinouchi K,Brown G,Pasternak G,Donner DB

更新日期：1991-12-31 00:00:00

abstract：:DNA topoisomerase II (Topo II) is an essential enzyme that catalyzes topological changes of DNA and consists of a major member of mitotic chromosomes. To investigate the dynamic localization of Topo II in nuclei, we engineered the strain of Aspergillus nidulans expressing Topo II fused with green fluorescent protein (...

journal_title：Biochemical and biophysical research communications

authors： Kawagishi M,Akashi T,Kikuchi A

更新日期：2005-08-26 00:00:00

abstract：:Although the neuron-restrictive silencer element (NRSE/Regard) has been shown to function as a negative-acting DNA regulatory element to prevent the expression of neuron-specific genes in non-neuronal cells, little is known about its silencing effect on transcription in primary glial cells nor its effect on transcript...

journal_title：Biochemical and biophysical research communications

authors： Tabuchi A,Nakatani C,Nakaoka R,Naruse Y,Kojima T,Mori N,Tsuda M

更新日期：1999-08-02 00:00:00

abstract：:Calmodulin contains multiple redox sensitive methionines whose oxidation alters the regulation of numerous targets. Molecular dynamics simulations were used to define the molecular principles that govern how calmodulin is structurally poised to detect and respond to methionine oxidation. We found that calmodulin's ope...

journal_title：Biochemical and biophysical research communications

authors： Walgenbach DG,Gregory AJ,Klein JC

更新日期：2018-10-20 00:00:00

abstract：:Negative regulator of differentiation 1 (Nrd1) is known as a negative regulator of sexual differentiation in fission yeast. Recently, it has been revealed that Nrd1 also regulates cytokinesis, in which physical separation of the cell is achieved by a contractile ring comprising many proteins including actin and myosin...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi A,Kanaba T,Satoh R,Fujiwara T,Ito Y,Sugiura R,Mishima M

更新日期：2013-07-19 00:00:00

abstract：:Chronic elevated glucose is harmful to pancreatic β-cells, resulting in pancreatic β-cells dysfunction and apoptosis. Understanding the molecular mechanisms associated with β-cells survival is pivotal for the prevention of β-cells injury caused by glucotoxicity. The role of Phosphatase and tensin homologue (PTEN)-indu...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Chen K,Wang L,Wan X,Shrestha C,Zhou J,Mo Z

更新日期：2016-08-05 00:00:00

abstract：:Casein kinase II (CKII) activity in colorectal tumours was compared before and after heterotransplantation onto nude mice. The test revealed that the enzyme activity was about two-fold enhanced in the tumours isolated from the nude mice when compared to the respective primary tumours from which they were derived. Immu...

journal_title：Biochemical and biophysical research communications

authors： Seitz G,Münstermann U,Schneider HR,Issinger OG

更新日期：1989-08-30 00:00:00

abstract：:In this study, we used, for the first time, atomic force microscope (AFM) images to investigate the mode of action of DNA topoisomerase I (topo I) in the presence and absence of its inhibitors: camptothecin (CPT) and tyrphostin AG-1387. The results revealed that in the absence of the inhibitors, the enzyme relaxed sup...

journal_title：Biochemical and biophysical research communications

authors： Argaman M,Bendetz-Nezer S,Matlis S,Segal S,Priel E

更新日期：2003-02-14 00:00:00

abstract：:Kinase suppressor of ras (KSR) and MEKK3 (MAP kinase kinase kinase) are integral members of the MAP kinase pathway. We have recently identified a new isoform of the KSR family named human kinase suppressor of ras-2 (hKSR-2), and demonstrated that hKSR-2 negatively regulates Cot, a MAP3K family member which is importan...

journal_title：Biochemical and biophysical research communications

authors： Channavajhala PL,Rao VR,Spaulding V,Lin LL,Zhang YG

更新日期：2005-09-09 00:00:00

abstract：:We explored the effect of transforming growth factor beta (TGF-beta), a cytokine that appears to play a central role in inflammatory events, on albumin expression by normal adult human hepatocytes and hepatoma cells. Addition of TGF-beta to primary human hepatocyte cultures resulted in a dramatic decrease in albumin a...

journal_title：Biochemical and biophysical research communications

authors： Busso N,Chesne C,Delers F,Morel F,Guillouzo A

更新日期：1990-09-14 00:00:00

abstract：:Previous studies have shown that DNA topoisomerase Iα (AtTOP1α) has specific developmental functions during growth and development in Arabidopsis thaliana. However, little is known about the roles of DNA topoisomerases in the closely related and commercially important plant, rapeseed (Brassica napus). Here, the full-l...

journal_title：Biochemical and biophysical research communications

authors： Gao C,Qi S,Liu K,Li D,Jin C,Duan S,Zhang M,Chen M

更新日期：2017-04-22 00:00:00

abstract：:A rabbit liver P-450-like pseudogene has been isolated from a lambda phage genomic library. Sequence analysis revealed structural homology with respect to the rat P-450b and P-450e genes as well as a similar intron-exon organization. A 5'-proximal TATA box-like sequence and two 3'-distal putative polyadenylation signa...

journal_title：Biochemical and biophysical research communications

authors： Zaphiropoulos PG,Folk WR,Coon MJ

更新日期：1986-01-29 00:00:00

abstract：:There are very few reports on antifungal proteins from rhizomes and there is none from the family of Zingiberaceae. An antifungal protein with a novel N-terminal sequence was isolated from ginger rhizomes utilizing a protocol that involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Ng TB

更新日期：2005-10-14 00:00:00

abstract：:The Shank family comprises three large multi-domain proteins playing central roles as protein scaffolds in the neuronal postsynaptic density. The Shank proteins are closely linked to neuropsychiatric diseases, such as autism spectrum disorders. One characteristic domain in the Shank family is the SH3 domain, assumed t...

journal_title：Biochemical and biophysical research communications

authors： Ponna SK,Myllykoski M,Boeckers TM,Kursula P

更新日期：2017-08-26 00:00:00

abstract：:CD40-mediated signals can induce cell aggregation, proliferation and rescue from apoptosis in WEHI231. To define which segment of cytoplasmic domain of CD40 and how signals are involved in those events, we generated mutant CD40 transfectants. We demonstrated the same 10 amino acid segment that could bind to tumor necr...

journal_title：Biochemical and biophysical research communications

authors： Hara T,Murakami M,Maeda H,Hibi M,Uede T

更新日期：1997-04-07 00:00:00

abstract：:The endoplasmic reticulum (ER) is an organelle in which most membrane and secretory proteins are synthesized. If these proteins are not folded correctly, unfolded proteins accumulate in the ER lumen, causing a cellular situation known as ER stress. Recently, many studies on the relationship between ER stress and disea...

journal_title：Biochemical and biophysical research communications

authors： Hosoda A,Maruyama A,Oikawa D,Oshima Y,Komachi Y,Kanai G,Sato H,Iwawaki T

更新日期：2011-02-04 00:00:00

abstract：:The extracellular Ca(2+) sensing receptor (CaSR) belongs to Class C G-protein-coupled receptors (GPCRs) which include receptors for amino acids, γ-aminobutyric acid and glutamate neurotransmitters. CaSR has been described as having an extended sequence containing a Ca(2+) binding pocket within an extracellular amino (...

journal_title：Biochemical and biophysical research communications

authors： Morrill GA,Kostellow AB,Gupta RK

更新日期：2015-03-27 00:00:00

abstract：:CD1d is a specific ligand for the invariant Valpha24Vbeta11-natural killer T (iNKT) cells that play an important role in placental development during early human pregnancy. The localization and regulation of placental CD1d expression remain unclear. Immunohistochemistry of human early gestational placentas revealed CD...

journal_title：Biochemical and biophysical research communications

authors： Matsumoto J,Kawana K,Nagamatsu T,Schust DJ,Fujii T,Sato H,Hyodo H,Yasugi T,Kozuma S,Taketani Y

更新日期：2008-06-27 00:00:00

abstract：:Number and molecular Brightness (N&B) analysis is a powerful method used to monitor protein aggregation in living cells. Here, we used the N&B method to characterize the unexpanded HTT protein oligomerization after the internalization of the mutant HTT (mHTT) which contains a CAG repeat extensions encoding for long po...

journal_title：Biochemical and biophysical research communications

authors： Sameni S,Zhang R,Digman MA

更新日期：2020-01-29 00:00:00

abstract：:The effect of vitamin A supplementation on stearoyl-CoA desaturase gene 1 expression in mouse liver was characterized. Normal BALB/c mice were fed 0.01% and 0.1% retinol palmitate as components of nonpurified diets. This treatment resulted in a 3-fold and a 7-fold induction of SCD1 mRNA levels, respectively, as determ...

journal_title：Biochemical and biophysical research communications

authors： Miller CW,Waters KM,Ntambi JM

更新日期：1997-02-03 00:00:00

abstract：:Oxysterol-binding protein-related proteins are implicated in the sensing and transporting lipids at the membrane contact sites. One of the members of the mammalian ORP family, ORP8, is thought to transport lipids through directly tethering both ER and PM membranes. Targeting to PM is thought to be mediated by N-termin...

journal_title：Biochemical and biophysical research communications

authors： Lee M,Fairn GD

更新日期：2018-02-19 00:00:00

abstract：:Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rap...

journal_title：Biochemical and biophysical research communications

authors： Bamba Y,Shofuda T,Kanematsu D,Nonaka M,Yamasaki M,Okano H,Kanemura Y

更新日期：2014-05-16 00:00:00

abstract：:A variety of different forms of cellular stress can cause protein misfolding and aggregation and proteotoxicity. The cytoprotective response to proteotoxicity is termed the integrated stress response and involves 4 distinct serine/threonine protein kinases that converge on the translation initiation factor eIF2α, resu...

journal_title：Biochemical and biophysical research communications

authors： McConkey DJ

更新日期：2017-01-15 00:00:00

abstract：:A novel protein methyltransferase has been recently described that catalyzes the esterification of the C-terminal leucine residue of the catalytic subunit of protein phosphatase 2A in a variety of eucaryotic cells. This reaction can potentially modulate the phosphatase's activity, subunit interactions, or interactions...

journal_title：Biochemical and biophysical research communications

authors： Xie H,Clarke S

更新日期：1994-09-30 00:00:00

abstract：:The present study examines whether and to what extent the profiles of proteolytic-related genes are altered in atrophying muscle during prolonged food deprivation. Long-term fasted animals exhibited three metabolic phases characterized by changes in lipid and protein utilization. Starvation induced an increase in some...

journal_title：Biochemical and biophysical research communications

authors： Bertile F,Le Maho Y,Raclot T

更新日期：2003-11-28 00:00:00