Abstract：
:DNA can be labeled with iodine in the reaction catalyzed with lactoperoxidase. Iodine is incorporated in cytosine similarly as in the chemical reaction. Iodination by means of the enzymatic method is faster than by means of the chemical method. The equilibrium state is still achieved after 10 minutes, during the enzymatic reaction, while during the chemical reaction it is achieved after 30 minutes. The preservation of the proper concentration of LP/DNA less than or equal to 0.075. ensures the proper reaction rate and decreases "self-iodination" of the enzyme. Deviations from the optimum conditions of the enzymatic reaction cause the decrease of the iodine incorporation or the increase of degradation of nucleic acid. Labeled DNA (less than or equal to 10% ICl) slightly differs from non-labeled DNA. Labeling 10% cytosine in DNA causes the decrease of melting temperature of about 1 degree C. DNA labeled this way reassociates with homologous non-labeled DNA at approximately 98%.
journal_name
Arch Immunol Ther Exp (Warsz)journal_title
Archivum immunologiae et therapiae experimentalisauthors
Gładek A,Mordarski Msubject
Has Abstractpub_date
1983-01-01 00:00:00pages
541-53issue
4eissn
0004-069Xissn
1661-4917journal_volume
31pub_type
杂志文章abstract：:We compared HLA antigen expression on new B-lymphoblastoid cell line (B-LCL) HAJ with that on B-LCLs expressing normal HLA levels as well as on B-LCLs derived from bare lymphocyte syndrome (BLS) patients and in vitro mutated B-LCLs of BLS-like phenotype. HAJ cells had no expression of HLA class II and low expression o...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Results of continuing studies on the influence of dimethylsulfoxide (DMSO) on reversion of sensitivity to rifampicin (RMP) and isonicotinic acid hydrazide (INH) in tubercle bacilli are reported. Nineteen strains of RMP-resistant bacilli, and 61 strains of IHN-resistant bacilli were studied. Reversion to INH sensitivit...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:The chicken is a foundational model for immunology research and continues to be a valuable animal for insights into immune function. In particular, the bursa of Fabricius can provide a useful experimental model of the development of B lymphocytes. Furthermore, an understanding of avian immunity has direct practical ap...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：:Comparison of pharmacological properties of commercial racemic Cyclophosphamide and its D- and L-enantiomers was performed in experiments on mice and rats. Acute toxicity, behavioral screening tests and the effects of subchronic treatment (influence on body mass, the increase of the mass of internal organs, mortality,...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:The studies concerned in vitro migration of peripheral blood lymphocytes of rabbits given intravenously one dose of E. coli lipopolysaccharide (LPS) and antipyretic doses of acetylsalicylic acid (ASA), indomethacin (IND) or mefenamic acid (MEFA). In normothermic animals, ASA appeared to inhibit in vitro the spontaneou...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:The effect of mice tissue homogenates on the inhibition of granulocyte-macrophage colony formation in vitro was studied. The potent inhibitory activity was displayed by homogenate from liver. The lowest values were found in the kidney and heart. An interrelationship between the rate of inhibition and protein concentra...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Studies on localization of structural differences between ovine and bovine serum and colostral IgG immunoglobulins are described. Comparison of heterogeneity, susceptibility to proteolytic enzymes, peptide maps, amino acid compositions, and antigenic properties of immunoglobulins and their Fab and Fc fragments and H a...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Allogenic, semisyngeneic and syngeneic sera of animals immunized with ML-positive leukemia L1210 cells, besides anti-ML antibodies, contain antibodies which react with Gross cellular surface antigen. ML antigen and Gross cellular surface antigen were shown by the immunoferritin test in electron microscopy, and by the ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:The purpose of this study is to evaluate the relationship between the concentration of interleukin-8 (IL-8) in exhaled breath condensate (EBC) and bronchoalveolar lavage fluid (BALF) with the disease activity score and pulmonary function of systemic lupus erythematosus (SLE) patients with and without pulmonary fibrosi...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：10.1007/s00005-014-0270-5
更新日期：2014-06-01 00:00:00
abstract：:Cytokines are small proteins or glycoproteins that transmit information from one cell to another. Most cells in the body secrete and respond to cytokines and their effects have been described on a myriad of cellular functions. Cytokine interactions may not be linear, thus making the system extremely intricate and with...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:In rheumatoid arthritis (RA), T cells infiltrate into the synovial membrane where they initiate and maintain activation of macrophages and synovial fibroblasts, transforming them into tissue-destructive effector cells. The diversity of the disease process and the formation of complex lymphoid microstructures indicate ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:The respiratory burst of polymorphonuclear leukocytes depends on activation of many enzymes and generation of variety of second messengers. One of important links leading to polymorphonuclear leukocytes (PMNL) activation is a transient rise in intracellular free calcium concentration ([Ca2+]i). Serine proteinase inhib...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Sodium salt of 9-oxo-10-acridineacetohydroxamic acid (HCA), a new synthetic compound, forms small crystals in aqueous solution. These crystals were easily phagocytized by the mouse bone marrow-derived macrophages. The ingested HCA crystals were visible under light microscope as dark granules. The degree of phagocytosi...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:The purpose of this study was to analyse the effect of various protocols of 15-deoxyspergualin (DOS) application on skin or kidney graft survival. Following rat strain combinations were used: AS-->LEW (MHC identical/non-MHC-different) and DA-->LEW (MHC-different/non-MHC-different). Reference DOS dose was 2.5 mg/kg, i....
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:The cytokine IL-15 performs numerous functions, such as promotion of growth and survival, on a plethora of cell types from both the lymphoid and non-lymphoid compartments. Therefore, mice genetically engineered to either lack or overexpress functional IL-15 display reduced immunological responses and leukemia, respect...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2005-03-01 00:00:00
abstract：:Until recently, the traditional view was that tolerance to self antigens was maintained by a combination of physical deletion, in the thymus, and functional deletion, in the periphery, of autoreactive T cells. There is now, however, abundant evidence that the normal T cell repertoire contains overtly autoreactive T ce...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:The mesenteric and pulmonary mast cells of guinea pigs were obtained by enzymatic dispersion of the tissues with the enzyme collagenase. The guinea pig mast cells obtained by this method were morphologically intact, as judged by light microscopy. The mast cells from lung and mesentery of actively sensitized guinea pig...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:The dry thermal glycation method was used to conjugate D-glucose and D-fructose with bovine serum albumin. Reactions were conducted at 50-104 degrees C for 30 min. Depending on temperature, different levels of substitution were achieved. In the case of D-glucose, average substitution levels of up to 53 mol glucose/mol...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The results of treatment with intrauterine transfusion are presented. The analysis comprised 48 successful transfusions into 29 foetuses. The reference group consisted of 11 transfusions which proved nonfeasible or ineffective and 54 untreated cases. Following blood treatment, the newborn mortality rate dropped more t...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:The effect of acute (single dose) and long-term ethanol treatment of Swiss Albino and C3H/He mice on IFN production stimulated in vitro by NDV, PHA and ConA was examined. Liver organ cultures and spleen cells of mice which received intraperitoneally a single dose (2 g/kg of body weight) of ethanol (II group) or were d...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The expression of class II antigens (HLA-DR, DP and DQ) on resting and PHA-activated T cells from renal allograft recipients was studied. A tendency for increase in DR+/DQ+ T cells was noted in azathioprine and cyclosporine-treated recipients undergoing rejection. Low inducible HLA-D expression (especially DP) was ass...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:The availability of neoplastic cell lines with well defined growth characteristics has greatly facilitated study of the tumor phenotype, tumor progression and metastatic process. MmB16 cell line has been established in vitro from the B16 mouse melanoma serially passaged in C57BL/6 mice. From MmB16 cells two lectin-res...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:This article takes issue with those who defend a brand of clinical research ethics that tends to substitute the ethics of clinical care of patients being recruited as trial subjects. The distinction between therapeutic and non-therapeutic studies is being disregarded by arguing that research is concerned with the purs...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：10.1007/s00005-009-0027-8
更新日期：2009-05-01 00:00:00
abstract：:1307 patients with suppurative bacterial infections caused by multidrug-resistant bacteria of different species were treated with specific bacteriophages (BP). BP therapy was highly effective; full recovery was noted in 1123 cases (85.9%). In 134 cases (10.9%) transient improvement was observed and only in 50 cases (3...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:The production of sheep Pseudomonas immune serum is described. The animals were immunized with Pseudomonas vaccine prepared from strains of Pseudomonas aeruginosa belonging to seven immunotypes of Fisher's schema. Immune sera obtained from hyperimmunized sheep are characterized by a high level of stabel antibodies wit...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Mast cells (MCs) are found widely distributed in tissues and contribute to regulation of inflammatory responses and ongoing modulation of the tissues. Although MCs are important in a variety of processes, including innate immunity, their role in allergic disease has received increasing attention in the past decade. MC...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：:In order to estimate the dependence of CD4 and CD8 antigen expression upon the cell activation, their presence on resting and activated human T cells was investigated. Peripheral blood lymphocytes of healthy donors derived from late (TEl) and early (TEe) E rosettes were used as resting and presumably in vivo activated...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The comparison of electrophoretic protein patterns and concentrations of the G, A and M immunoglobulins before initiation of oral IFN-alpha therapy of viral hepatitis and after 2 weeks of treatment allow to predict patient's response. Patient who after 2 weeks of treatment with ultra-low orally administered IFN-alpha ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Biofilms are probably one of the most common structures formed by microorganisms in various environments. The higher resistance of such microbial communities to stress conditions, including antibiotics and host immune response, is recently extensively studied. However, the weak activity of phagocytic cells against mic...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：10.1007/s00005-013-0240-3
更新日期：2013-10-01 00:00:00
abstract：:The central action and LD50 of 11 new 2,5-substituted derivates of tetrahydropirimidinedione-4,6 with an aryl group at C2 were investigated. The most favorable action was exerted by 2-furfurylamine derivatives with an alkil or benzyl group at C5. These compounds acted in doses of 0.0025--0.01 of their LD50 synergistic...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
