当前位置： SCI文献检索 > PHYTOCHEMISTRY期刊下所有文献 > Alpha-amylase from mung beans (Vigna radiata)--correlation of biochemical properties and tertiary structure by homology modelling.

Alpha-amylase from mung beans (Vigna radiata)--correlation of biochemical properties and tertiary structure by homology modelling.

Abstract：

:Alpha-amylase from germinated mung beans (Vigna radiata) has been purified 600-fold to electrophoretic homogeneity and a final specific activity of 437 U/mg. SDS-PAGE of the final preparation revealed a single protein band of 46 kDa. The optimum pH was 5.6. The energy of activation was determined to be 7.03 kcal/mol in the temperature range 15-55 degrees C. Km for starch was 1.6 mg/mL in 50 mM sodium acetate buffer, pH 5.5. Thermal inactivation studies at 70 degrees C showed first-order kinetics with rate constant (k) equal to 0.005 min(-1). Mung bean alpha-amylase showed high specificity for its primary substrate starch. Addition of EDTA (10 mM) caused irreversible loss of activity. Mung bean alpha-amylase is inhibited in a non-competitive manner by heavy metal ions, for example, mercury with a Ki of 110 microM. Homology modelling studies with mung bean alpha-amylase using barley alpha-amylases Amy 1 and Amy 2 as templates showed a very similar structure as expected from the high sequence identity. The model showed that alpha-amylase from mung beans has no sugar-binding site, instead it has a methionine. Furthermore, instead of two tryptophans, it has Val(277) and Lys(278), which are the conserved residues, important for proper folding and conformational stability.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Tripathi P,Lo Leggio L,Mansfeld J,Ulbrich-Hofmann R,Kayastha AM

doi

10.1016/j.phytochem.2007.04.006

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

1623-31

issue

12

eissn

0031-9422

issn

1873-3700

pii

S0031-9422(07)00239-7

journal_volume

68

pub_type

杂志文章

相关文献

PHYTOCHEMISTRY文献大全
  • NMR characterization of hydration and thermal stress in tomato fruit cuticles.

    abstract：:In its natural environment, the plant cuticle, which is composed of the biopolymer cutin and a mixture of surface and embedded cuticular waxes, experiences a wide variety of temperatures and hydration states. Consequently, a complete understanding of cuticular function requires study of its thermal and mechanical prop...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2008.08.016

    authors： Stark RE,Yan B,Stanley-Fernandez SM,Chen ZJ,Garbow JR

    更新日期：2008-11-01 00:00:00

  • Differential antibiosis against Helicoverpa armigera exerted by distinct inhibitory repeat domains of Capsicum annuum proteinase inhibitors.

    abstract：:Plant defensive serine proteinase inhibitors (PIs) are known to have negative impact on digestive physiology of herbivore insects and thus have a crucial role in plant protection. Here, we have assessed the efficacy and specificity of three previously characterized inhibitory repeat domain (IRD) variants from Capsicum...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2014.01.011

    authors： Joshi RS,Gupta VS,Giri AP

    更新日期：2014-05-01 00:00:00

  • Isolation and synthesis of chalcones with different degrees of saturation.

    abstract：:Crotaoprostrin, a chalcone not yet known as a plant constituent, was isolated from the aerial parts of the Indian medicinal plant Crotalaria prostrata. The structures of the chalcone polyarvin and the partially hydrogenated naturally occurring derivatives crotaramin, crotaramosmin, and crotin were confirmed by chemica...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(02)00475-2

    authors： Krohn K,Steingröver K,Srinivasa Rao M

    更新日期：2002-12-01 00:00:00

  • Occurrence and characterization of oils rich in gamma-linolenic acid (III): the taxonomical value of the fatty acids in Echium (Boraginaceae).

    abstract：:Fourteen species of the genus Echium (Fam. Boraginaceae) collected in the Macaronesia were surveyed in a search for high levels of gamma-linolenic acid (GLA, 18:3omega6) in the seed oil. High amounts of this fatty acid were found in all of them, ranging from 18.85% (E. pitardii var. pitardii) to 27.42% (E. gentianoide...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(01)00184-4

    authors： Guil-Guerrero JL,Gómez-Mercado F,Rodríguez-García I,Campra-Madrid P,García-Maroto F

    更新日期：2001-09-01 00:00:00

  • Amino acid sequence of the 26 kDa subunit of legumin-type seed storage protein of common buckwheat (Fagopyrum esculentum Moench): molecular characterization and phylogenetic analysis.

    abstract：:The paper describes the amino acid sequence of a 26 kDa basic subunit of 13S globulin of common buckwheat (Fagopyrum esculentum Moench). The protein has 93 and 75% sequence homology with 11S globulin of Coffea arabica and beta subunit of 11S globulin of Cucurbita pepo respectively. The subunit has the "globally conser...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(02)00755-0

    authors： Bharali S,Chrungoo NK

    更新日期：2003-05-01 00:00:00

  • Conjugates of auxin and cytokinin.

    abstract：:Plant growth and developmental processes as well as environmental responses require the action and cross talk of phytohormones including auxins and cytokinins. Active phytohormones are changed into multiple forms by acylation, esterification or glycosylation, for example. It seems that conjugated compounds could serve...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2009.05.006

    authors： Bajguz A,Piotrowska A

    更新日期：2009-05-01 00:00:00

  • Ambiguanine A-G, hexahydrobenzophenanthridine alkaloids from Corydalis ambigua var. amurensis.

    abstract：:Seven hexahydrobenzophenanthridine-type alkaloids, Ambiguanine A-G, along with eight known alkaloids, were isolated from tubers of Corydalis ambigua var. amurensis. Their structures were elucidated based on extensive spectroscopic analyses, with absolute configurations determined by CD experiments. ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2014.05.013

    authors： Yang ZY,Jiang BH,Teng FK,Han N,Liu ZH,Zhou JJ,Wang Y,Guo DA,Yin J

    更新日期：2014-09-01 00:00:00

  • Enantiomeric β-carboline dimers from Picrasma quassioides and their anti-hepatoma potential.

    abstract：:Four pairs of enantiomeric β-carboline alkaloids, (+/-)-kumudine A-D, along with their biosynthesis-related compound kumudine E, were obtained from the stems of Picrasma quassioides. Their structures, including the absolute configurations, were determined via extensive spectroscopic data combined with electronic circu...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2018.12.002

    authors： Zhao WY,Zhou WY,Chen JJ,Yao GD,Lin B,Wang XB,Huang XX,Song SJ

    更新日期：2019-03-01 00:00:00

  • Phenylpropanoid glucosides from leaves of Coussarea hydrangeifolia (Rubiaceae).

    abstract：:Phenylpropanoid glycosides, 1'-O-benzyl-alpha-L-rhamnopyranosyl-(1''-->6')-beta-D-glucopyranoside (1) and alpha-L-xylopyranosyl-(4''-->2')-(3-O-beta-D-glucopyranosyl)-1'-O-E-caffeoyl-beta-D-glucopyranoside (2), together with the known derivatives, 1,6-di-O-caffeoyl-beta-D-glucopyranoside (3), 1-O-(E)-caffeoyl-beta-D-g...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2005.06.019

    authors： Hamerski L,Bomm MD,Silva DH,Young MC,Furlan M,Eberlin MN,Castro-Gamboa I,Cavalheiro AJ,da Silva Bolzani V

    更新日期：2005-08-01 00:00:00

  • Two cationic peroxidases from cell walls of Araucaria araucana seeds.

    abstract：:We have previously reported the purification and partial characterization of two cationic peroxidases from the cell walls of seeds and seedlings of the South American conifer, Araucaria araucana. In this work, we have studied the amino acid composition and NH2-terminal sequences of both enzymes. We also compare the da...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/0031-9422(94)00665-g

    authors： Riquelme A,Cardemil L

    更新日期：1995-05-01 00:00:00

  • Euphane triterpenoids of Cassipourea lanceolata from the Madagascar rainforest.

    abstract：:Fractionation of an ethanol extract of a Madagascar collection of the leaves and fruit of Cassipourea lanceolata Tul. led to the isolation of three euphane triterpenoids 1-3. The (1)H and (13)C NMR spectra of all compounds were fully assigned using a combination of 2D NMR experiments, including COSY, TOCSY, HSQC (HMQC...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2009.12.009

    authors： Hou Y,Cao S,Brodie PJ,Miller JS,Birkinshaw C,Andrianjafy MN,Andriantsiferana R,Rasamison VE,TenDyke K,Shen Y,Suh EM,Kingston DG

    更新日期：2010-04-01 00:00:00

  • Anti-inflammatory lignans and phenylethanoid glycosides from the root of Isodon ternifolius (D.Don) Kudô.

    abstract：:Five undescribed lignans, three undescribed phenylethanoid glycosides and eight known compounds were isolated from the root of Isodon ternifolius (D.Don) Kudô (Lamiaceae). The structures of all of the isolated constituents were characterized by physical data analyses including NMR, MS and ECD. The anti-inflammatory ac...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2018.05.017

    authors： Zhang Y,Wang K,Chen H,He R,Cai R,Li J,Zhou D,Liu W,Huang X,Yang R,Deng S,Li J,Guan X

    更新日期：2018-09-01 00:00:00

  • Deposition of stearate-oleate rich seed fat in Mangifera indica is mediated by a FatA type acyl-ACP thioesterase.

    abstract：:Although the mechanism of accumulation of C8-C16 saturated fatty acids in seed oils has been well-studied, the control of stearic (C18:0) acid deposition in high stearate seed fat is still unclear. We investigated the mechanism that regulates high level of stearate and oleate (C18:1) accumulation in mango (Mangifera i...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2010.11.004

    authors： Bhattacharjee A,Ghosh SK,Neogi K,Aich A,Willard B,Kinter M,Sen SK,Ghosh D,Ghosh S

    更新日期：2011-02-01 00:00:00

  • Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris).

    abstract：:Non-targeted LC-MS metabolomics on fruit of three wild and domesticated apple species (Malus sylvestris, M. sieversii and M. domestica) showed that two crab apple (M. sylvestris) accessions were distinguished by high concentrations of an ascorbic acid glycoside (AAG). This was partly purified, but key NMR signals were...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2020.112297

    authors： Richardson AT,Cho J,McGhie TK,Larsen DS,Schaffer RJ,Espley RV,Perry NB

    更新日期：2020-05-01 00:00:00

  • Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants.

    abstract：:The multitude of terpene carbon skeletons in plants is formed by enzymes known as terpene synthases. This review covers the monoterpene and sesquiterpene synthases presenting an up-to-date list of enzymes reported and evidence for their ability to form multiple products. The reaction mechanisms of these enzyme classes...

    journal_title：Phytochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.phytochem.2009.07.030

    authors： Degenhardt J,Köllner TG,Gershenzon J

    更新日期：2009-10-01 00:00:00

  • Profiling of spatial metabolite distributions in wheat leaves under normal and nitrate limiting conditions.

    abstract：:The control and interaction between nitrogen and carbon assimilatory pathways is essential in both photosynthetic and non-photosynthetic tissue in order to support metabolic processes without compromising growth. Physiological differences between the basal and mature region of wheat (Triticum aestivum) primary leaves ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2015.01.007

    authors： Allwood JW,Chandra S,Xu Y,Dunn WB,Correa E,Hopkins L,Goodacre R,Tobin AK,Bowsher CG

    更新日期：2015-07-01 00:00:00

  • Chemotaxonomy of the Oleaceae: iridoids as taxonomic markers.

    abstract：:The distribution and biosynthesis of iridoid glucosides in the Oleaceae is reviewed and five distinct biosynthetic pathways to iridoids have been identified in the family, deoxyloganic acid apparently being a common intermediate. Likewise, the distributions of caffeoyl phenylethanoid glycosides (CPGs), i.e. verbascosi...

    journal_title：Phytochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/s0031-9422(02)00102-4

    authors： Jensen SR,Franzyk H,Wallander E

    更新日期：2002-06-01 00:00:00

  • Scrokoelziside A, a triterpene glycoside from Scrophularia koelzii.

    abstract：:Scrokoelziside A, isolated from the aerial parts of Scrophularia koelzii was shown to be 3-O-([alpha-L-rhamnopyranosyl-(1-->3), beta-D-glucopyranosyl-(1-->2)]-beta-D-fucopyranosyl-(1-->4)-beta-D- glucopyranosyl]-13 beta,28-epoxyolean-11-en-23-ol, on the basis one- and two-dimensional NMR homo- and hetero-nuclear spect...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/0031-9422(95)00697-4

    authors： Bhandari SP,Roy R,Agrawal PK,Garg HS

    更新日期：1996-02-01 00:00:00

  • Structure and stereochemistry of dimeric proteracacinidins possessing the rare C-4(C) --> C-5(D) interflavanyl linkage.

    abstract：:The rare series of (4-->5)-linked proteracacinidins is extended by identification of oritin-(4alpha-->5)-epioritin-4beta-ol, ent-epioritin-(4alpha-->5)-epioritin-4beta-ol, epioritin-(4beta-->5)-epioritin-4alpha-ol and ent-oritin-(4beta-->5)-epioritin-4alpha-ol from the heartwoods of Acacia galpinii and Acacia caffra. ...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(02)00010-9

    authors： Bennie L,Coetzee J,Malan E,Ferreira D

    更新日期：2002-03-01 00:00:00

  • Characterisation of the willow phenylalanine ammonia-lyase (PAL) gene family reveals expression differences compared with poplar.

    abstract：:Willow is an important biomass crop for the bioenergy industry, and therefore optimal growth with minimal effects of biotic and abiotic stress is essential. The phenylpropanoid pathway is responsible for the biosynthesis of not only lignin but also of flavonoids, condensed tannins, benzenoids and phenolic glycosides w...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2015.06.005

    authors： de Jong F,Hanley SJ,Beale MH,Karp A

    更新日期：2015-09-01 00:00:00

  • Dammarane-type triterpenoid saponins from Salvia russellii Benth.

    abstract：:Three undescribed dammarane-type saponins, russelliinosides A-C, together with a common sterol (β-sitosterol), an abietane diterpenoid (18-hydroxyferruginol), two oleane triterpenoids (daturaolone and oleanolic acid), an ursane triterpenoid (ursolic acid) as well as three 5-hydroxyflavones (cirsimaritin, eupatorin, an...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2020.112653

    authors： Hafez Ghoran S,Firuzi O,Asadollahi M,Stuppner H,Alilou M,Jassbi AR

    更新日期：2021-01-29 00:00:00

  • Purification and properties of pi-repressible acid phosphatases from Aspergillus nidulans.

    abstract：:Two forms of the pacA-encoded acid phosphatase (designated acid phosphatases I and II) secreted by the mold Aspergillus nidulans grown in low-Pi medium at 37 degrees, pH5.0, were purified to apparent homogeneity by PAGE. The M(r) of the purified enzyme forms were ca 115000 (60000) and 113000 (62000) respectively for f...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(98)00205-2

    authors： Nozawa SR,MacCheroni W Jr,Stábeli RG,Thedei G Jr,Rossi A

    更新日期：1998-11-20 00:00:00

  • Galactinol synthase enzyme activity influences raffinose family oligosaccharides (RFO) accumulation in developing chickpea (Cicer arietinum L.) seeds.

    abstract：:To understand raffinose family oligosaccharides (RFO) metabolism in chickpea (Cicer arietinum L.) seeds, RFO accumulation and corresponding biosynthetic enzymes activities were determined during seed development of chickpea genotypes with contrasting RFO concentrations. RFO concentration in mature seeds was found as a...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2016.02.009

    authors： Gangola MP,Jaiswal S,Kannan U,Gaur PM,Båga M,Chibbar RN

    更新日期：2016-05-01 00:00:00

  • Pithedulosides A-G, oleanane glycosides from Pithecellobium dulce.

    abstract：:Seven saponins named pithedulosides A-G were isolated from the seeds of Pithecellobium dulce. Their structures were established through spectral analyses as echinocystic acid 3-O-alpha-L-arabinopyranosyl-(1-->6)-beta-D-glucopyranoside, echinocystic acid and oleanolic acid 3-O-alpha-L-arabinopyranosyl- (1-->2)-alpha-L-...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(96)00725-x

    authors： Nigam SK,Gopal M,Uddin R,Yoshikawa K,Kawamoto M,Arihara S

    更新日期：1997-04-01 00:00:00

  • A bidesmosidic triterpene glycoside from the roots of Symphytum officinale.

    abstract：:A new bidesmosidic triterpenoidal saponin of hederagenin was isolated from the ethanolic extract of the roots of Symphytum officinale L. Its structure was elucidated by using 1H NMR, 1H-1H COSY NMR, heteronuclear 1H-13C correlated spectroscopy (heteroCOSY), 13C NMR, DEPT, FAB mass spectrometry and chemical evidence as...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(00)97091-2

    authors： Noorwala M,Mohammad FV,Ahmad VU,Sener B

    更新日期：1994-05-01 00:00:00

  • Carotene derivatives in sexual communication of zygomycete fungi.

    abstract：:Recognition between mating partners, early sexual morphogenesis and development are regulated by a family of beta-carotene derived signal compounds, the trisporoids, in zygomycete fungi. Mating type-specific precursors are released from the hyphae and exert their physiological effects upon compatible mating partners. ...

    journal_title：Phytochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.phytochem.2009.07.014

    authors： Schimek C,Wöstemeyer J

    更新日期：2009-10-01 00:00:00

  • 3 alpha-hydroxy-oleanane-type triterpene glycosyl esters from leaves of Acanthopanax spinosus.

    abstract：:Three novel 3 alpha-hydroxy-oleanane-type triterpene oligoglycosyl esters, spinoside C1, C4 and C5, were isolated from the leaves of Acanthopanax spinosus. The structures were established to be 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranosyl esters of 3 alpha, 29-dihydroxy-olean...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(00)90853-7

    authors： Miyakoshi M,Ida Y,Isoda S,Shoji J

    更新日期：1993-12-01 00:00:00

  • alpha-D-Glucuronosyl-(1-->3)-L-galactose, an unusual disaccharide from polysaccharides of the hornwort Anthoceros caucasicus.

    abstract：:Acid hydrolysis of cell wall-rich material from thalli of the hornwort Anthoceros caucasicus yielded substantial amounts of an unusual disaccharide (1). Hydrolysis of 1 yielded only GlcA, Gal and unhydrolysed 1. Compound 1 was identified as alpha-D-GlcpA-(1-->3)-L-Gal by 1H and 13C NMR spectroscopic analysis and by th...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(03)00330-3

    authors： Popper ZA,Sadler IH,Fry SC

    更新日期：2003-09-01 00:00:00

  • COX-2 inhibitory activity of homoisoflavanones and xanthones from the bulbs of the Southern African Ledebouria socialis and Ledebouria ovatifolia (Hyacinthaceae: Hyacinthoideae).

    abstract：:The bulbs of Ledebouria socialis (Hyacinthaceae) yielded the benzocyclobutene homoisoflavonoid, (R)-2',5-dihydroxy-3',4',7-trimethoxyspiro{2H-1-benzopyran-3-(4H)-9-bicyclo[4.2.0]octa[1,3,5]triene}-4-one, socialinone (1). Ledebouria ovatifolia yielded (2ε,3R)-2,5-dihydroxy-7-methoxyspiro[2H-1-benzopyran-3(4H), 5'(6'H)-...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/j.phytochem.2013.06.024

    authors： Waller CP,Thumser AE,Langat MK,Crouch NR,Mulholland DA

    更新日期：2013-11-01 00:00:00

  • Production of bioactive triterpenes by Eriobotrya japonica calli.

    abstract：:Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2alpha-hydoxyursolic acid, maslinic acid, tormentic acid, 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oi...

    journal_title：Phytochemistry

    pub_type： 杂志文章

    doi：10.1016/s0031-9422(01)00455-1

    authors： Taniguchi S,Imayoshi Y,Kobayashi E,Takamatsu Y,Ito H,Hatano T,Sakagami H,Tokuda H,Nishino H,Sugita D,Shimura S,Yoshida T

    更新日期：2002-02-01 00:00:00