Abstract：
:RNA was labeled with iodine in the reaction catalyzed with lactoperoxidase (LP). Labeling of RNA requires additional purification of LP on Sephadex G-100 and application of RNase inhibitors. Immobilized LP shows lower activity than a soluble enzyme but still it is sufficient to label RNA to 7% IC. Immobilized enzyme is easy to remove from the post-reaction mixture and can be used many times. Optimum conditions for enzymatic labeling were as follows: concentration ratio C/KI for the soluble enzyme equal to 7.5 (for the immobilized enzyme--12). The oxidizing agent (H2O2) used in the enzymatic method of labeling at the concentrations required in the reaction (3.5 X 10(-5) M) does not cause the degradation of nucleic acids as opposed to the oxidizing agent (TlCl3) used in the chemical method. The degree of RNA degradation increases with the amount of incorporated iodine. The RNA preparations of high degree of labeling (10% iodocytosine) by means of the chemical and enzymatic methods do not differ much. At lower labeling the enzymatic method produces less degraded preparations.
journal_name
Arch Immunol Ther Exp (Warsz)journal_title
Archivum immunologiae et therapiae experimentalisauthors
Gładek A,Mordarski Msubject
Has Abstractpub_date
1983-01-01 00:00:00pages
555-63issue
4eissn
0004-069Xissn
1661-4917journal_volume
31pub_type
杂志文章abstract：:The intraventricular administration of histamine in a dose of 500 microgram, and two histamine H2-receptor agonists i.e. 50-250 microgram of dimaprit and 100 microgram of 4-methylhistamine decreased GABA level after 30 min (by approximately 20%) in the hypothalamus, but not in the striatum of the rat brain. The level ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription complex which plays a crucial role in cellular adaptation to low oxygen availability. In the last years there has been increasing evidence about the role of this factor in inflammatory/innate immune reactions. It has also been found to contribute to d...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-010-0083-0
更新日期：2010-08-01 00:00:00
abstract：:It was demonstrated that exogenous GH suppressed the resistance to L. monocytogenes infection in Listeria resistant C57Bl/6 and susceptible A/J mice. However, different parameters of the immunological reaction to Listeria were affected by GH treatment in these mouse strains. In C57Bl/6 mice GH decreased accumulation o...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Antiinflammatory effect of ibuprofen was studied in the model of adjuvant arthritis in rats. Its effectiveness was evaluated on the basis of morphological and biochemical indices. The values of proteolytic and beta-glucuronidase activity in the blood serum were shown to correlate well with clinical exacerbation of adj...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Heat-killed cell suspension of several Propionibacterium acnes strains were prepared and studied for their protective activity in viral infection of mice and immunomodulating properties. Majority of the strains caused marked spleen enlargement in the treated mice. These changes persisted for several weeks. Only some o...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Different infections are the most common complication of immunosuppressive therapy. In this context, the effect of cyclosporine A (CsA) on the innate antiviral immunity of mice was studied. The presence of immunity was shown by infection of resident peritoneal cells (RPC) of BALB/c mice with herpes simplex virus type ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:CEA and NCA show an increased mobility in SDS-polyacrylamide gel electrophoresis if the samples are preheated in 1% SDS for 5 min at 100 degrees C. Now we found that the slow-to-fast form conversion of CEA and NCA also occurs under milder conditions and is reversible at low SDS concentration. At 100 degrees C the conv...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common human progressive dementia linked, in its sporadic form, to a common polymorphism within a gene for apolipoprotein E (APOE) mapped to chromosome 19. Individuals with APOE 4 isoforms are more prone to develop sporadic from of AD than those who carry APOE 2 or APOE 3 alleles. ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Programmed cell death protein 1 (PD-1) and its ligand PD-L1 are critical for the regulation of T cell exhaustion and activity suppression. Tumor cells expressing immune checkpoints including PD-L1 escape monitoring of T cells from the host immune system. Checkpoint inhibitors are highly promising therapies that functi...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-020-00601-6
更新日期：2020-11-13 00:00:00
abstract：:Nicotinic acetylcholine receptors (nAChRs) are membrane ligand-gated cation channels whose activation is triggered by the binding of the endogenous neurotransmitter acetylcholine or other biologic compounds including nicotine. Their roles in synaptic transmission in the central and peripheral nervous system as well as...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-013-0266-6
更新日期：2014-04-01 00:00:00
abstract：:Sphingosine 1-phosphate (S1P) has been shown to be a bioactive lipid mediator intimately involved in mediating a variety of immunological processes. In particular, S1P regulates lymphocyte cell trafficking between the lymphatic system and the blood. The lysophospholipid signals mainly through five related G protein-co...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：10.1007/s00005-006-0028-9
更新日期：2006-07-01 00:00:00
abstract：:The chemotactic activity of elastin-derived peptides (EP) for human polymorphonuclear leukocytes (PMNL) was investigated using the under agarose method. The EP were produced by digesting the bovine ligament elastin with porcine pancreatic elastase. Thus prepared digest had weak chemotactic activity for PMNL. The mean ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Dimeric hybrid protein molecule consisting of disulfide-linked beta (heavy) subunit of haptoglobin (Hp) and L (light) subunit of immunoglobulin G, was synthesized. Molecular mass of the hybrid was 62.0 kd, sedimentation constant B20 = 2.43. Hemoglobin binding activity of the beta X L hybrid as compared with that of na...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:The investigation was carried on mice infected with influenza A/053/74/H3N2 virus. 129/Ao/Boy, inbred mice, were inoculated intranasally with influenza virus. The alveolar and peritoneal cells from infected and uninfected animals were induced in vitro with Newcastle disease virus. It was shown that the alveolar cells ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:Pharmacological activity of 6 newly synthetized water-soluble derivatives of barbiturylo propanosulfonic acids, was evaluated. The compounds were obtained under the conditions of the Ritter reaction. The experiments were performed with the aim to determine the anti-inflammatory and immunotropic activities as well as t...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Clinical trials have shown that the type I interferon (IFN)-alpha/beta have some beneficial effects on organ-specific autoimmune diseases, such as Behcet's diseases and multiple sclerosis, although the precise mechanisms remain largely unresolved. T helper cells 1 (Th1)-mediated autoimmune responses are involved in th...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:The mesenteric and pulmonary mast cells of guinea pigs were obtained by enzymatic dispersion of the tissues with the enzyme collagenase. The guinea pig mast cells obtained by this method were morphologically intact, as judged by light microscopy. The mast cells from lung and mesentery of actively sensitized guinea pig...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:In a previous study, anticataleptic action of cyproheptadine was reported. The present investigation deals with the influence of WA-335 (9,10-dihydro-10-(1-methyl-4-piperidylidene)-9-anthrol), another antagonist of serotonin, on catalepsy induced in rats with spiroperidol, pimozide, fluphenazine and reserpine. WA-335 ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:Human chorionic gonadotropin--subunit beta (beta-hCG) has been so far most extensively studied antigen for immuno-contraceptive properties. Studies of vaccination of non-human primates has been so far controversially reported since some antisera obtained from baboons immunized with beta-hCG had shown cross-reactivity ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：1990-01-01 00:00:00
abstract：:The object of this paper is the existence of LH/hCG receptors in female uterine tissue. Specific and high-affinity binding sites for LH are present in the pig and rabbit uterus. Estradiol promoted the synthesis of LH receptors in porcine myometrium. Stimulation of LH receptors with hCG in estrogen-primed tissue had a ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：1990-01-01 00:00:00
abstract：:Total number of mast cells in popliteal lymph nodes of Balb/c mice is a function of time and Nu gene (complex?) content. The number of mast cells increases with age - in both wild +/+ and in Nu gene (complex?) carriers (Nu/+ and Nu/Nu). The increase in the number of mast cells is faster in the Nu gene carriers than in...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:After several decades of research into the macrophage migration inhibitory factor (MIF), its diverse actions in the immune system are yet to be fully revealed. What has become clear is that MIF plays an important role in both innate and adaptive immunity. However, while several pathways mediating the function of MIF i...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2004-11-01 00:00:00
abstract：:Bovine (BLF) and human (HLF) lactoferrin inhibit proliferation and cytokine production by an antigen specific TH1 cell line stimulated with antigen presenting cells (APC) and antigen. Beside the inhibitory effects on TH1 cells we observed a decrease of interleukin 2 receptor (IL-2R) expression on these cells contacted...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:In our previous studies we found that the slower growth of syngeneic, immunogenic MC-induced sarcoma (MC-Sa) in aging rats was followed by the higher activity of spleen lymphocytes in ADCC assay. The purpose of the present paper was to study infiltrating cells of immunogenic and non-immunogenic MC-Sa and to estimate i...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The central action and LD50 of 11 new 2,5-substituted derivates of tetrahydropirimidinedione-4,6 with an aryl group at C2 were investigated. The most favorable action was exerted by 2-furfurylamine derivatives with an alkil or benzyl group at C5. These compounds acted in doses of 0.0025--0.01 of their LD50 synergistic...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:A preliminary study of six hairy cell leukemia patients treated with one course of 2-chloro-2'-deoxyadenosine (2-CdA) is presented. 2-CdA was administered 0.1 mg/kg/daily by intravenous infusion over 7 days. Two patients achieved CR and four PR. ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:In rheumatoid arthritis (RA), T cells infiltrate into the synovial membrane where they initiate and maintain activation of macrophages and synovial fibroblasts, transforming them into tissue-destructive effector cells. The diversity of the disease process and the formation of complex lymphoid microstructures indicate ...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:Mast cells (MCs) are found widely distributed in tissues and contribute to regulation of inflammatory responses and ongoing modulation of the tissues. Although MCs are important in a variety of processes, including innate immunity, their role in allergic disease has received increasing attention in the past decade. MC...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：:A highly statistically significant correlation was found between asbestosis and impaired responsiveness in skin reaction to intermediate and second strength tuberculin and SK-SD. Considering ANA incidence these antibodies were found with higher frequency in asbestos workers who lacked a cutaneous response to the recal...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:The studies concerned in vitro migration of peripheral blood lymphocytes of rabbits given intravenously one dose of E. coli lipopolysaccharide (LPS) and antipyretic doses of acetylsalicylic acid (ASA), indomethacin (IND) or mefenamic acid (MEFA). In normothermic animals, ASA appeared to inhibit in vitro the spontaneou...
journal_title：Archivum immunologiae et therapiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
