Abstract:
:CEA and NCA show an increased mobility in SDS-polyacrylamide gel electrophoresis if the samples are preheated in 1% SDS for 5 min at 100 degrees C. Now we found that the slow-to-fast form conversion of CEA and NCA also occurs under milder conditions and is reversible at low SDS concentration. At 100 degrees C the conversion was completed within few minutes in 0.03% SDS, while at lower temperatures it required higher SDS concentration and longer time of incubation. After the antigens were heated at 100 degrees C at 0.05% or lower SDS concentration, they showed slow reassociation at room temperature. Under conditions insufficient for the complete slow-to-fast or fast-to-slow form conversion two CEA or NCA bands were present at various proportions. No material with an intermediate mobility was detected for CEA, while a weak transient intermediate band (located closer to the fast band) was visible in some gels showing an incomplete dissociation or association of NCA. The ratio of mol. wts. of slow and fast band was approx. 2:1 for NCA and 1.5:1 for CEA. Despite some deviations from the expected mol. wt. of the presumably undissociated CEA, the results obtained supplied a support for the conclusion that the two electrophoretic forms of CEA and NCA represent the dimer-monomer interconversion.
journal_name
Arch Immunol Ther Exp (Warsz)journal_title
Archivum immunologiae et therapiae experimentalisauthors
Krop-Watorek A,Sedlaczek P,Lisowska Esubject
Has Abstractpub_date
1989-01-01 00:00:00pages
703-14issue
5-6eissn
0004-069Xissn
1661-4917journal_volume
37pub_type
杂志文章abstract::The dry thermal glycation method was used to conjugate D-glucose and D-fructose with bovine serum albumin. Reactions were conducted at 50-104 degrees C for 30 min. Depending on temperature, different levels of substitution were achieved. In the case of D-glucose, average substitution levels of up to 53 mol glucose/mol...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The production of sheep Pseudomonas immune serum is described. The animals were immunized with Pseudomonas vaccine prepared from strains of Pseudomonas aeruginosa belonging to seven immunotypes of Fisher's schema. Immune sera obtained from hyperimmunized sheep are characterized by a high level of stabel antibodies wit...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
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更新日期:1981-01-01 00:00:00
abstract::Preclinical pharmacological studies of two acridine derivatives, dihydrochloride N10-oxide 1-nitro-9-/3-dimethylaminopropylamino/-acridine (C-666) and dihydrochloride 1-nitro-9-[(2-dimethylamino)-1-methylethylamino]-acridine (C-829) are reported. Both compounds are characterized by biological activity, poor absorption...
journal_title:Archivum immunologiae et therapiae experimentalis
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更新日期:1979-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章,评审
doi:10.1007/s00005-014-0285-y
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abstract::Glycogen-protein complexes from the muscles of Celerio euphorbiae and horse as well as from the livers of frog, carp and man were isolated and used as antigens. After immunization of rabbits antisera were obtained from which fractions of immunoglobulins were isolated. Antibodies were used in agar immunodiffusion tests...
journal_title:Archivum immunologiae et therapiae experimentalis
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更新日期:1986-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Studies performed on chimpanzees and humans have revealed that strong, multispecific and sustained CD4(+) and CD8(+) T cell immune responses is a major determinant of hepatitis C virus (HCV) clearance. However, spontaneous elimination of the virus occurs in minority of infected individuals and cellular response direct...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章,评审
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:10.1007/s00005-018-0511-0
更新日期:2018-10-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::To evaluate the mesenchymal stem cells (MSCs) influence on cytokines and matrix metalloproteinases (MMPs) expression in rat bladder wall regeneration. MSCs cultures from the bone marrow were established. Acellular matrices from the bladder submucosa were prepared. Bladders were reconstructed using cell-seeded (n = 5) ...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:10.1007/s00005-013-0249-7
更新日期:2013-12-01 00:00:00
abstract::A line of AKR leukemic mouse cells cultured in vitro, designated AKR 87, was established and characterized. Two types of cells were distinguished in this line fibroblasts and lymphoblasts. The lymphoblast-like cells resembled the original tumor cells spontaneous leukemia of AKR mice. In the XC test, cells of this line...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
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更新日期:1976-01-01 00:00:00
abstract::The availability of neoplastic cell lines with well defined growth characteristics has greatly facilitated study of the tumor phenotype, tumor progression and metastatic process. MmB16 cell line has been established in vitro from the B16 mouse melanoma serially passaged in C57BL/6 mice. From MmB16 cells two lectin-res...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::The aim of this study was to analyze association between HLA class I antigens and sarcoidosis in Poland. HLA-A, B, C antigens in a group of 100 patients suffering from sarcoidosis and in a group of 100 healthy blood donors were determined. Histocompatibility typing was performed by the NIH method using commercially av...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::The original article has been published without acknowledgment section. The acknowledgement section is given below for your reading. ...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00005-017-0490-6
更新日期:2017-12-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章,评审
doi:10.1007/s00005-014-0269-y
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
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更新日期:2000-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章,评审
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更新日期:1997-01-01 00:00:00
abstract::There is a strong epidemiologic relationship between chronic hepatitis C virus (HCV) infection and the development of hepatocellular carcinoma, although the cellular and molecular mechanisms of tumor formation remain to be firmly established. Clearly, HCV is associated with the development of chronic hepatitis and cir...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章,评审
doi:
更新日期:2001-01-01 00:00:00
abstract::Trasylol in a single dose 100000 i.u. was administered intravenously to 22 patients suffering from bronchial asthma. Pulmonary function was evaluated by the subjects self-comfort, physical examination, spirometric measurements (VC, FEV1, FEV1%, MCBind.) and blood gas analysis (pH PCO2, PO2, SaO2). In half of the patie...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Lyt phenotypes of interleukin-2 and interferon producing Balb/c cortisone resistant thymocytes, stimulated by concanavalin A in vitro, were studied using cytotoxic anti-Lyt-1.2 and anti-Lyt-2.2 alloantisera and monoclonal antibodies. It was found that while interleukin-2 was produced by both Lyt1+ and Lyt2+ cells, the...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章,评审
doi:10.1007/s00005-007-0037-3
更新日期:2007-09-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:10.1007/s00005-016-0423-9
更新日期:2017-10-01 00:00:00
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journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::Clinical trials have shown that the type I interferon (IFN)-alpha/beta have some beneficial effects on organ-specific autoimmune diseases, such as Behcet's diseases and multiple sclerosis, although the precise mechanisms remain largely unresolved. T helper cells 1 (Th1)-mediated autoimmune responses are involved in th...
journal_title:Archivum immunologiae et therapiae experimentalis
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00