Abstract:
:A method is described for growing monolayers of newborn rat beta-cells in microculture trays. After disruption of the pancreas with collagenase, islets were isolated by Ficoll density gradient centrifugation, trypsinized to obtain individual cells, and plated in 96-well tissue culture trays. The cells were incubated for the first 3 days in growth medium containing 0.1 mM 3-isobutyl-1-methylxanthine to promote monolayer formation. The cultures could be maintained in a functional state, as defined by their responsiveness to known modulators of insulin secretion, for at least 2 weeks. As few as 1 X 10(3) islet cells/well gave results that were reproducible within +/- 10%. It is suggested that the microculture system for islet cells might prove to be a rapid and reproducible screening technique for studying drugs, viruses, or other agents that affect beta-cell function.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Dobersen MJ,Scharff JE,Notkins ALdoi
10.1210/endo-106-4-1070subject
Has Abstractpub_date
1980-04-01 00:00:00pages
1070-3issue
4eissn
0013-7227issn
1945-7170journal_volume
106pub_type
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