Abstract:
:7B2 is a novel neuroendocrine polypeptide which belongs to an entirely new superfamily of proteins. In extension of previous reports on 7B2, these studies concern its expression in endocrine pancreatic tissue. They have been performed using specific antibodies prepared against two distinct synthetic fragments of 7B2 comprising amino acids 23-39 and 117-128 of the native human molecule isolated from pituitary gland. Pancreatic insulin-secreting tumors produced in transgenic mice contain high amounts of 21,500- to 22,000-dalton forms of 7B2. Using light microscopy (immunocytochemical colocalization with different pancreatic hormones), immunoreactivity to 7B2 (IR-7B2) was consistently found within cells producing insulin and glucagon and less consistently within pancreatic polypeptide-containing cells. As in previous reports concerning the brain, adenohypophysis, and thyroid gland, IR-7B2 could be detected by electron microscopy within secretory granules of alpha- and beta-like cells in islets. Furthermore, the IR-7B2 level was higher in extracts of insulin-producing tumors of the transgenic mice that contained the hybrid insulin II gene. In addition, IR-7B2 could be detected immunocytochemically in three of seven tumors produced in the rat by streptozotocin-nicotinamide treatment.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Benjannet S,Marcinkiewicz M,Falgueyret JP,Johnson DE,Seidah NG,Chrétien Mdoi
10.1210/endo-123-2-874subject
Has Abstractpub_date
1988-08-01 00:00:00pages
874-84issue
2eissn
0013-7227issn
1945-7170journal_volume
123pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The importance of locally produced insulin-like growth factor-I (IGF-I) in connective tissues has recently been recognized. It has been postulated that the action of anabolic hormones on bone may be mediated through local IGF-I release. However, whether IGF-I can also be modulated by other locally acting cytokines has...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-4-1635
更新日期:1990-10-01 00:00:00
abstract::The incidence of obesity, with its impact on the development of comorbidities, including diabetes and cardiovascular disease, represents one of the greatest global health threats of the 21st century. This is particularly damning considering the vast progress that has been made in understanding the factors and molecula...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2016-1944
更新日期:2017-05-01 00:00:00
abstract::During in vitro maturation of porcine cumulus cell-oocyte complexes and in vitro luteinization of porcine granulosa cells, FSH induces the expression of the protease TNFα-converting enzyme/A disintegrin and metalloproteinase domain 17 (TACE/ADAM17) and the epidermal growth factor (EGF)-like factors, which activate the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1655
更新日期:2014-03-01 00:00:00
abstract::The participation of growth factors in wound healing and tissue repair has been well established. Previous studies demonstrated that the expression of keratinocyte growth factor (KGF) was greatly elevated shortly after injury and that topical application of KGF accelerated healing. Steroidal antiinflammatory agents, s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.6.8641170
更新日期:1996-06-01 00:00:00
abstract::Melanocortin receptor 4 (MCR4) and CB(1) cannabinoid receptors independently modulate food intake. Although an interaction between the cannabinoid and melanocortin systems has been found in recovery from hemorrhagic shock, the interaction between these systems in modulating food intake has not yet been examined. The p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0059
更新日期:2004-07-01 00:00:00
abstract::Japanese quail, a species with a precocial pattern of development, were used as a model for studying the ontogeny of thyroid responses to TSH. Thiourea was administered to embryos early in incubation, and the effects were assessed by measuring thyroid 32P uptake, serum thyroid hormones, thyroid weights, and body weigh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-4-1238
更新日期:1984-04-01 00:00:00
abstract::A chemiluminescent dot blot assay has been developed by our laboratory for rapid determinations of IGF-I in serum-free conditioned media (CM) collected from cultured cells. In contrast to IGF-I radioimmunoassays (RIAs), the IGF binding proteins (IGFBPs) did not interfere with the dot blot assay and did not require the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5237
更新日期:1997-05-01 00:00:00
abstract::Basal or hypothalamic extract-induced ACTH or TSH secretory capacity as well as intracellular ACTH content were assessed in cultured rat adenohypophyseal cells after various pretreatments of the donor animals. Medium and intracellular ACTH or TSH were measured by radioimmunoassay. Ten-day to 2-month adrenalectomy enha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-3-717
更新日期:1976-03-01 00:00:00
abstract::Although mothers against dpp (MAD) and its related proteins (MADR) are believed to be important components of the cell signaling pathway for the transforming growth factor beta (TGFbeta) superfamily, the presence and regulation of these signaling molecules in ovarian cells by TGFbeta is not known. In the present studi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.9.5376
更新日期:1997-09-01 00:00:00
abstract::The effect of a potent agonistic LHRH analog D-Trp-6-LHRH on the hyperprolactinemia induced by haloperidol was tested in intact and ovariectomized female rats. The administration of D-Trp-6-LHRH at two dose levels (5 and 50 micrograms/day) for 20 days blocked the increase in serum PRL induced by haloperidol in intact ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2227
更新日期:1985-06-01 00:00:00
abstract::To elucidate the roles of the hypothalamic peptides, GH-releasing hormone (GRH) and somatostatin (SRIH), potentially responsible for altered GH dynamics in diabetes, we studied the time courses of their changes in level associated with altered GH secretion in streptozotocin (STZ)-induced diabetic mice. Diabetic mice w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7664670
更新日期:1995-10-01 00:00:00
abstract::Aside from studies of a possible synergism between T3 and glucocorticoids in regulating GH mRNA, there have been few previous studies of the modulation by hormones and other factors of glucocorticoid hormone regulation of pituitary cell GH gene expression. We have employed a serum-free medium containing no exogenously...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-1986
更新日期:1987-05-01 00:00:00
abstract::Trans-10, cis-12 conjugated linoleic acid (10,12 CLA) is a dietary fatty acid that promotes weight loss and disproportionate fat loss. Obese mice fed a high-fat, high-sucrose (HFHS) diet containing 10,12 CLA are resistant to weight gain and contain markedly reduced subcutaneous fat and adiponectin, with a concurrent l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00213
更新日期:2017-09-01 00:00:00
abstract::The actin-binding protein filamin A (FLNa) affects the intracellular trafficking of various classes of receptors and has a potential role in oncogenesis. However, it is unclear whether FLNa regulates the signaling capacity and/or down-regulation of the activated epidermal growth factor receptor (EGFR). Here it is show...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1344
更新日期:2009-06-01 00:00:00
abstract::Mice with somatotrope-specific deletion of the Janus kinase binding site in leptin receptors are GH deficient as young adults and become obese by 6 months of age. This study focused on the metabolic status of young (3-4.5 month old) preobese mutant mice. These mutants had normal body weights, lean body mass, serum lep...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1331
更新日期:2012-10-01 00:00:00
abstract::The rat anterior pituitary expresses beta(2)-adrenoceptors (ARs) on somatotrophs, lactotrophs, and corticotrophs. The present study investigates whether beta(1)-ARs exist in the anterior pituitary, in which cell type(s) they are found, and whether they are regulated by glucocorticoids. As determined by quantitative RT...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1396
更新日期:2008-05-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF-alpha) has been shown to induce insulin resistance in cultured cells as well as in animal models. The aim of this study was to map the in vivo mechanism whereby TNF-alpha contributes to the pathogenesis of impaired insulin signaling, using obese and lean Zucker rats in which TNF-alpha ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.12.6336
更新日期:1998-12-01 00:00:00
abstract::Dysregulation of the hypothalamic-pituitary-adrenocortical axis has been theoretically linked to the processes of aging for decades. To investigate the effects of age on high frequency rhythms of plasma ACTH at the time of circadian activation, integrated 2-min blood samples were collected over 4 h in 10 young and 14 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8393770
更新日期:1993-08-01 00:00:00
abstract::The possible role of the pentose phosphate shunt in thyroid hormone secretion was investigated in vivo and in vitro with mouse thyroid glands. Thyroidal endocytosis in response to TSH, a step of thyroid hormone secretion, was evaluated for its dependency upon the pentose phosphate shunt by using 6-aminonicotinamide (6...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-4-962
更新日期:1975-10-01 00:00:00
abstract::The comparative effectiveness of vitamin D3 and its derivatives in curing hyperparathyroidism and osteodystrophic bone lesions was examined in a laboratory model of renal osteodystrophy associated with marked secondary hyperparathyroidism in rats. The experimental model was prepared by a single injection of homologous...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-1-328
更新日期:1980-07-01 00:00:00
abstract::We have measured the concentrations of vasopressin in sequential samples of cerebrospinal fluid (CSF) obtained from fetal lambs between 108 and 130 days gestation. There was a clear and significant (F = 4.46; P less than 0.005) rhythm in vasopressin concentrations in the CSF of nine fetuses, characterized by a mean tr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-3095
更新日期:1989-06-01 00:00:00
abstract::This study was undertaken to identify uterine insulin-like growth factor-I (IGF-I) receptors and examine the regulation of these receptors throughout the estrous cycle and after 17 beta-estradiol (E2) administration to immature rats. We have demonstrated type I IGF receptors in crude uterine membranes by binding and c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-597
更新日期:1989-08-01 00:00:00
abstract::Insulin-like growth factors I and II (IGF-I and -II) are polypeptides secreted by skeletal cells and are considered regulators of bone formation. IGF-I and -II were studied for their effects on collagen synthesis and degradation in cultures of intact fetal rat calvariae and on type I collagen transcript levels in oste...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-301
更新日期:1989-01-01 00:00:00
abstract::Steroid hormones can differentially modify gonadotropin release stimulated by GnRH. Decreased GnRH-mediated gonadotropin release has been observed in vitro after pretreatment with androgens or glucocorticoids. In this study, we tested this phenomenon further with the use of a new cytochemical stain for a potent biotin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-1-396
更新日期:1985-07-01 00:00:00
abstract::Thyroid hormone entry into the thymocyte, a thyroid hormone target, was investigated by incubating the cells with tracer amounts of [125I]L-T3. At 37 C T3 uptake was linear with time up to 2 min, and then approached a plateau. The specific T3 uptake, obtained by subtracting the uptake in the presence of excess unlabel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2443
更新日期:1989-05-01 00:00:00
abstract::Lanosterol 14alpha-demethylase (CYP51) responds to cholesterol feedback regulation through sterol regulatory element binding proteins (SREBPs). The proximal promoter of CYP51 contains a conserved region with clustered regulatory elements: GC box, cAMP-response elements (CRE-like), and sterol regulatory element (SRE). ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0781
更新日期:2005-12-01 00:00:00
abstract::Benign prostatic hyperplasia (BPH) is an age-related debilitating prostatic disease that is frequently associated with prostatic inflammation and bothersome lower urinary tract symptoms (LUTS). Animal models have shown that formalin- and bacterial-induced prostatic inflammation can induce bladder dysfunction; however,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa212
更新日期:2021-01-01 00:00:00
abstract::The developmental appearance and regulation of hypothalamic vasopressin (prepropressophysin) mRNA was studied using quantitated in situ hybridization techniques. Vasopressin mRNA levels in the supraoptic nuclei were reliably detected on day 16 of gestation, while mRNA in the suprachiasmatic nuclei (SCN) was detectable...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2483
更新日期:1987-06-01 00:00:00
abstract::To evaluate the relative contribution of the GH/IGF axis to the development of peak bone mineral density (BMD), we measured skeletal changes in IGF-I knockout (KO), IGF-II KO, and GH-deficient lit/lit mice and their corresponding control mice at d 23 (prepubertal), 31 (pubertal), and 56 (postpubertal) in the entire fe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220948
更新日期:2003-03-01 00:00:00
abstract::Puberty is associated with an increase in the plasma concentration of sex steroids, GH, and insulin-like growth factor-I (IGF-I). Gonadal steroid hormones are important for the normal pubertal growth spurt and skeletal growth. The mechanism by which gonadal steroids induce skeletal growth is still not fully understood...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6618
更新日期:1999-04-01 00:00:00