Abstract:
:1 Because normal human blood platelets contain higher concentrations of taurine than any other amino acid, and have a platelet: plasma concentration gradient exceeding 400: 1, we isolated the cells in vitro and incubated them with radioactively labelled taurine in order to investigate the existence of a metabolically-dependent accumulation process.2 Platelets incubated with taurine (1 to 100 nmol/ml) in autologous plasma or Krebs solution accumulated [(14)C]-taurine against the concentration gradient.3 The transport process was saturable at high concentrations, showed a requirement for sodium ions, and was temperature-dependent.4 The kinetics of transport fulfilled the criteria of Michaelis & Menten for saturable enzyme/substrate interactions, but the kinetic constants were influenced by the incubation medium.5 The metabolic inhibitors 2,4-dinitrophenol and iodoacetic acid in combination inhibited taurine transport in Krebs solution, but stimulated transport in autologous plasma. The latter result suggested the involvement of a sodium-dependent ATPase in taurine transport.6 We conclude that platelets actively transport taurine in vitro under experimental conditions closely resembling those likely to occur in vivo, and that this taurine transport process may be involved in the maintenance of the platelet: plasma concentration gradient.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Ahtee L,Boullin DJ,Paasonen MKdoi
10.1111/j.1476-5381.1974.tb09707.xsubject
Has Abstractpub_date
1974-10-01 00:00:00pages
245-51issue
2eissn
0007-1188issn
1476-5381journal_volume
52pub_type
杂志文章abstract::1. To elucidate whether K+ channels play a role in the action of epithelium-dependent bronchodilatation, we studied responses in human bronchial strips in the presence of indomethacin and NG-nitro-L-arginine methylester under isometric conditions, in vitro. 2. Mechanical removal of the epithelium increased the contrac...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1038/sj.bjp.0701183
更新日期:1997-06-01 00:00:00
abstract::1. (+)-Tubocurarine, hexamethonium, atropine, ouabain, and removal of potassium from the bathing medium were examined for their effects on indirectly evoked twitches (IT) of mouse phrenic nerve-diaphragm muscles in the presence or absence of neostigmine. 2. Neostigmine increased the amplitude of IT. The twitch potenti...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1989.tb11798.x
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Inhibition of the T- and B-cell interaction through the CD40/CD40 ligand (L) axis is a favourable approach for inflammatory disease treatment. Clinical studies of anti-CD40L molecules in autoimmune diseases have met challenges because of thromboembolic events and adverse haemostasis. VIB4920 (for...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/bph.14897
更新日期:2020-03-01 00:00:00
abstract::1. A crude preparation of toxin was extracted from a sample of mussels Mytilus edulis, part of a batch responsible for many cases of paralytic shellfish poisoning.2. The crude extract was partially purified by absorption on sodium Amberlite ion-exchange resin. Two toxins were recovered by elution from the Amberlite, a...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1970.tb10660.x
更新日期:1970-12-01 00:00:00
abstract::1. We have characterized the 5-hydroxytryptamine (5-HT)-induced calcium signalling in endothelial cells from the human pulmonary artery. Using RT-PCR we show, that of all cloned G-protein coupled 5-HT receptors, these cells express only 5-HT1D beta, 5-HT2B and little 5-HT4 receptor mRNA. 2. In endothelial cells 5-HT i...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1996.tb16700.x
更新日期:1996-03-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 临床试验,杂志文章
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1038/sj.bjp.0707180
更新日期:2007-05-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1995.tb16368.x
更新日期:1995-06-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1986.tb10246.x
更新日期:1986-07-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2007-11-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1991.tb12275.x
更新日期:1991-04-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Exposure to an acute stress inhibits gastric emptying and stimulates colonic transit via central neuropeptide Y (NPY) pathways; however, peripheral involvement is uncertain. The anxiogenic phenotype of NPY(-/-) mice is gender-dependent, raising the possibility that stress-induced gastrointestinal...
journal_title:British journal of pharmacology
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更新日期:2012-08-01 00:00:00
abstract::1. Intraplantar administration of the nitric oxide (NO) donor, sodium nitroprusside (SNP), induces hyperaemia in the rat paw skin, which is in part due to release of calcitonin gene-related peptide (CGRP) from afferent nerve fibres. The present study examined whether prostaglandins or other inflammatory mediators part...
journal_title:British journal of pharmacology
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更新日期:1995-11-01 00:00:00
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journal_title:British journal of pharmacology
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:2020-12-07 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1476-5381.1976.tb07689.x
更新日期:1976-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-03-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1993-09-01 00:00:00
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更新日期:1973-07-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2019-11-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1996.tb15198.x
更新日期:1996-01-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
abstract::The consumption of green tea (Camellia sinensis) has been shown to have many physiological and pharmacological health benefits. In the past two decades several studies have reported that epigallocatechin-3-gallate (EGCG), the main constituent of green tea, has anti-infective properties. Antiviral activities of EGCG wi...
journal_title:British journal of pharmacology
pub_type: 杂志文章,评审
doi:10.1111/bph.12009
更新日期:2013-03-01 00:00:00