Abstract：
:1. To elucidate whether K+ channels play a role in the action of epithelium-dependent bronchodilatation, we studied responses in human bronchial strips in the presence of indomethacin and NG-nitro-L-arginine methylester under isometric conditions, in vitro. 2. Mechanical removal of the epithelium increased the contractile responses to acetylcholine; the pD2 values increased from 5.0 +/- 0.2 to 5.9 +/- 0.3 (P < 0.001). This potentiation was abolished by iberiotoxin but not by apamin or glibenclamide. 3. In cascade bioassay, application of the bathing medium from dispersed, bronchial epithelial cells to epithelium-denuded bronchial strips decreased acetylcholine-induced contraction by 44 +/- 6%. This effect was reduced to 10 +/- 3% (P < 0.01) when the epithelial cells were pretreated with iberiotoxin, and to 4 +/- 1% (P < 0.001) when the epithelial cells were incubated with Ca(2+)-free medium containing [1,2-bis(2) aminophenoxy] ethane N,N,N',N'-tetraacetic acid-acetomethoxy ester. 4. In contrast, the bronchodilator effect of the medium bathing epithelial cells was not altered by the direct addition of iberiotoxin to epithelium-denuded tissues. 5. These results suggest that the Ca(2+)-activated K+ channel may play a role in the synthesis and/or release of smooth muscle relaxing factor, which is neither nitric oxide nor a cyclo-oxygenase product, from airway epithelial cells.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Tamaoki J,Tagaya E,Isono K,Kondo M,Konno Kdoi
10.1038/sj.bjp.0701183subject
Has Abstractpub_date
1997-06-01 00:00:00pages
794-8issue
4eissn
0007-1188issn
1476-5381journal_volume
121pub_type
杂志文章abstract：:1. The pharmacological characteristics of RP 67580, a non-peptide tachykinin NK1 receptor antagonist, and its effects on a reflex response evoked by stimulation of primary afferent fibres, were examined in isolated neonatal spinal cord preparations of the rat. Potentials were recorded extracellularly from a lumbar ven...
journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1994.tb17116.x
更新日期：1994-12-01 00:00:00
abstract：:1. Alprenolol, a beta-adrenoceptor blocking drug reported to have the same potency as propranolol in vitro and in vivo, was found to be four times more active than procaine as a local anaesthetic on frog sciatic nerve.2. At doses of 0.125 mg/kg and above alprenolol protected anaesthetized guinea-pigs against ouabain-i...
journal_title：British journal of pharmacology
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journal_title：British journal of pharmacology
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pub_type： 杂志文章
doi：10.1038/sj.bjp.0701797
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pub_type： 杂志文章
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更新日期：1983-03-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 评论,杂志文章
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更新日期：2009-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1038/sj.bjp.0705854
更新日期：2004-11-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1990.tb12945.x
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journal_title：British journal of pharmacology
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更新日期：2005-10-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1111/bph.15209
更新日期：2020-07-22 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1993.tb13779.x
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pub_type： 杂志文章
doi：10.1111/bph.12594
更新日期：2014-07-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/bph.15084
更新日期：2020-08-01 00:00:00
