Abstract:
:1. The prostate function is regulated by androgens and alpha-adrenergic activity. Clinically, antiandrogens and/or alpha(1)-adrenergic antagonists are commonly used to treat symptomatic prostatic hypertrophy. To elucidate the effects of androgen deprivation on prostate contractility via alpha(1)-adrenoceptor, the characteristics and expression of alpha(1)-adrenoceptors were examined in castrated rats. 2. Isolated prostate strips from intact and castrated rats were subjected to a phenylephrine stimulated contraction. Prazosin (10 nM), [(3)H]-prazosin and phenoxybenzamine (3 - 300 nM) were used for inhibition assay, receptor characterization and partial alkylation of alpha-adrenoceptor, respectively. The mRNA content of three subtypes of alpha-adrenoceptors was determined by reverse transcription combined with polymerase chain reaction (RT - PCR). 3. Contractile response to phenylephrine increased in castrated rats, which could be explained by a relative increase of the stromal component. A lowered contraction potency was also noted in castrated rats. Receptor binding assay indicated minimal changes in the affinity or density of alpha(1)-adrenoceptor. Escalating alkylation of the alpha(1)-adrenoceptor population resulted in a rightward shift in the contraction-response curves before depressing maximal contractile force, and the suppression was detected at lower doses in castrated rats. RT - PCR study confirmed the expression of three types of alpha(1)-adrenoceptor, alpha(1a), alpha(1b) and alpha(1d)-adrenoceptors, in intact rat prostate, and revealed that alpha(1a)-adrenoceptor, but not alpha(1b) or alpha(1d)-adrenoceptors, was down-regulated in castrates. 4. The results show that androgen deprivation suppressed alpha(1)-adrenergic contractility of rat prostate strips, and the suppression was associated with down-regulation of receptor reserve for the alpha(1a)-adreneroceptor population expressed in intact rat prostate.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Homma Y,Hamada K,Nakayama Y,Tsujimoto G,Kawabe Kdoi
10.1038/sj.bjp.0703706subject
Has Abstractpub_date
2000-12-01 00:00:00pages
1454-60issue
7eissn
0007-1188issn
1476-5381journal_volume
131pub_type
杂志文章abstract::1. The effects of the lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA) on the ionic currents of rat carotid body type I cells were investigated by use of whole-cell and outside-out patch clamp techniques. 2. NDGA (5-50 microM) produced a concentration-dependent inhibition of whole-cell K+ currents at all activa...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1038/sj.bjp.0701452
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doi:10.1038/sj.bjp.0702517
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1038/sj.bjp.0706208
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journal_title:British journal of pharmacology
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abstract:BACKGROUND AND PURPOSE:Hydrogen sulphide reduces uterine contractility and is of potential interest as a treatment for uterine disorders. The aim of this study was to explore the mechanism of sodium sulphide (Na2 S)-induced relaxation of rat uterus, investigate the importance of redox effects and ion channel-mediated m...
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更新日期:2015-07-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
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更新日期:1983-01-01 00:00:00
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更新日期:2004-09-01 00:00:00
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更新日期:1998-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjp.0703499
更新日期:2000-08-01 00:00:00
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doi:10.1038/sj.bjp.0706843
更新日期:2006-10-01 00:00:00
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更新日期:2010-10-01 00:00:00
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更新日期:2020-11-03 00:00:00
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journal_title:British journal of pharmacology
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更新日期:2013-08-01 00:00:00
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更新日期:2000-06-01 00:00:00
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doi:10.1038/sj.bjp.0701324
更新日期:1997-08-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:2001-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1476-5381.1991.tb12402.x
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pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjp.0703172
更新日期:2000-03-01 00:00:00
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doi:10.1111/j.1476-5381.1991.tb12448.x
更新日期:1991-10-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1983.tb08809.x
更新日期:1983-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1476-5381.1975.tb07363.x
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