Abstract：
:1. The effects of the lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA) on the ionic currents of rat carotid body type I cells were investigated by use of whole-cell and outside-out patch clamp techniques. 2. NDGA (5-50 microM) produced a concentration-dependent inhibition of whole-cell K+ currents at all activating test potentials (holding potential -70 mV). The time-course of the inhibition was also concentration-dependent and the effects of NDGA were only reversible following brief periods of exposure (<2 min). Another lipoxygenase inhibitor, phenidone (5 microM), was without effect on whole-cell K+ currents in carotid body type I cells. 3. NDGA (5-50 microM) also inhibited whole-cell Ca2+ channel currents (recorded with Ba2+ as charge carrier) in a concentration-dependent manner. 4. Isolation of voltage-gated K+ channels by use of high [Mg2+] (6 mM), low [Ca2+] (0.1 mM) solutions revealed a direct inhibition of the voltage-sensitive component of the whole-cell K+ current by NDGA (50 microM). 5. In excised, outside-out patches NDGA (20-50 microM) increased large conductance, Ca2+ activated K+ channel activity approximately 10 fold, an effect which could be reversed by either tetraethylammonium (10 mM) or charybdotoxin (30 nM). 6. It is concluded that NDGA activates maxi-K+ channels in carotid body type I cells and over the same concentration range inhibits voltage-sensitive K+ and Ca2+ channels. The inhibition of whole cell K+ currents seen is most likely due to a combination of direct inhibition of the voltage-sensitive K+ current and indirect inhibition of maxi-K+ channel activity through blockade of Ca2+ channels.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Hatton CJ,Peers Cdoi
10.1038/sj.bjp.0701452subject
Has Abstractpub_date
1997-11-01 00:00:00pages
923-9issue
5eissn
0007-1188issn
1476-5381journal_volume
122pub_type
杂志文章abstract：:The properties of the ATPase released during electrical field stimulation (EFS) (8 Hz, 25 s) of the sympathetic nerves of the superfused rabbit isolated vas deferens were investigated. Superfusate collected during EFS rapidly metabolised exogenous ATP (100 microM) and 50% was broken down in 5.67+/-0.65 min. The main m...
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doi：10.1038/sj.bjp.0703662
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doi：10.1038/sj.bjp.0705869
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journal_title：British journal of pharmacology
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doi：10.1111/j.1476-5381.1996.tb16700.x
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1996.tb15270.x
更新日期：1996-03-01 00:00:00
abstract：BACKGROUND AND PURPOSE:Inhibitors of phosphodiesterase 5 (PDE5) affect signalling pathways by elevating cGMP, which is a second messenger involved in processes of neuroplasticity. In the present study, the effects of the PDE5 inhibitor, sildenafil, on the pathological features of Alzheimer's disease and on memory-relat...
journal_title：British journal of pharmacology
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pub_type： 杂志文章
doi：10.1111/j.1476-5381.1995.tb13362.x
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abstract：:A large range of neuroadaptations develop in response to chronic opioid exposure and these are thought to be more or less critical for expression of the major features of opioid addiction: tolerance, withdrawal and processes that may contribute to compulsive use and relapse. This review considers these adaptations at ...
journal_title：British journal of pharmacology
pub_type： 杂志文章,评审
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更新日期：2008-05-01 00:00:00
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doi：10.1038/sj.bjp.0701183
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pub_type： 杂志文章
doi：10.1111/j.1476-5381.1973.tb08350.x
更新日期：1973-07-01 00:00:00
abstract：:1 The recently discovered benzodiazepine receptor exists in high concentration in the cerebral cortex. We have, therefore, examined the effects of diazepam and chlordiazepoxide on cortical neurone responses to excitatory and inhibitory amino acids and acetylcholine, in the cortex of rats anaesthetized with urethane.2 ...
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doi：10.1111/j.1476-5381.1979.tb08702.x
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pub_type： 杂志文章
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journal_title：British journal of pharmacology
pub_type： 杂志文章,评审
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pub_type： 杂志文章
doi：10.1038/sj.bjp.0705263
更新日期：2003-05-01 00:00:00
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journal_title：British journal of pharmacology
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doi：10.1111/j.1476-5381.1980.tb07028.x
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journal_title：British journal of pharmacology
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doi：10.1111/j.1476-5381.1977.tb08424.x
更新日期：1977-11-01 00:00:00
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journal_title：British journal of pharmacology
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更新日期：1980-01-01 00:00:00
abstract：:The aim of the present study was to determine whether inhibition of cyclic nucleotide phosphodiesterase (PDE) modulates the stimulated generation of the cytokines, interleukin-4 (IL-4) and IL-13, from human basophils. This was addressed by evaluating the effects of both nonselective and selective inhibitors of PDEs on...
journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1038/sj.bjp.0705892
更新日期：2004-08-01 00:00:00
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更新日期：1993-12-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/bph.12690
更新日期：2014-07-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1992.tb14265.x
更新日期：1992-02-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1986.tb10176.x
更新日期：1986-01-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1994.tb14814.x
更新日期：1994-03-01 00:00:00
