Abstract:
:We report the first optical recordings of action potentials, in single trials, from one or a few (approximately 1-2 microm) mammalian nerve terminals in an intact in vitro preparation, the mouse neurohypophysis. The measurements used two-photon excitation along the "blue" edge of the two-photon absorption spectrum of di-3-ANEPPDHQ (a fluorescent voltage-sensitive naphthyl styryl-pyridinium dye), and epifluorescence detection, a configuration that is critical for noninvasive recording of electrical activity from intact brains. Single-trial recordings of action potentials exhibited signal-to-noise ratios of approximately 5:1 and fractional fluorescence changes of up to approximately 10%. This method, by virtue of its optical sectioning capability, deep tissue penetration, and efficient epifluorescence detection, offers clear advantages over linear, as well as other nonlinear optical techniques used to monitor voltage changes in localized neuronal regions, and provides an alternative to invasive electrode arrays for studying neuronal systems in vivo.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Fisher JA,Barchi JR,Welle CG,Kim GH,Kosterin P,Obaid AL,Yodh AG,Contreras D,Salzberg BMdoi
10.1152/jn.00929.2007subject
Has Abstractpub_date
2008-03-01 00:00:00pages
1545-53issue
3eissn
0022-3077issn
1522-1598pii
00929.2007journal_volume
99pub_type
杂志文章abstract::The aim of this study was to investigate the contribution of feedback from large-diameter sensory fibers to the adaptation of soleus muscle activity after small ankle trajectory modifications during human walking. Small-amplitude and slow-velocity ankle dorsiflexion enhancements and reductions were applied during the ...
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