Abstract:
:Phosphatidylinositol specific phospholipase C from Staphylococcus aureus could solubilize acetylcholinesterase up to 55% from sheep platelets in the presence of ethylenediaminetetra acetic acid (EDTA). The endogenous phosphatidylinositol specific phospholipase C of platelets activated by deoxycholate (at 3-5 mM) could also solubilize the enzyme to a similar extent. The solubilized enzyme could be further purified to apparent homogeneity by affinity chromatography without the use of any detergents. It is suggested that phosphatidylinositol specific phospholipase C will be a useful tool in the solubilization of acetylcholinesterase from mammalian sources and its purification free of detergents. The present study also demonstrates the parallel behaviour of acetylcholinesterase and aryl acylamidase in platelets confirming their identity.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Majumdar R,Balasubramanian ASdoi
10.1016/0006-2952(85)90202-3subject
Has Abstractpub_date
1985-12-01 00:00:00pages
4109-15issue
23eissn
0006-2952issn
1873-2968pii
0006-2952(85)90202-3journal_volume
34pub_type
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pub_type: 杂志文章
doi:10.1016/j.bcp.2004.06.020
更新日期:2004-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1988-12-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/j.bcp.2005.07.031
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2000-08-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2000-03-15 00:00:00
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pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章,评审
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更新日期:1996-04-26 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章,评审
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更新日期:2018-09-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(86)90686-6
更新日期:1986-12-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/s0006-2952(97)00204-9
更新日期:1997-10-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/j.bcp.2017.05.001
更新日期:2017-08-15 00:00:00