当前位置： SCI文献检索 > BIOCHEMICAL PHARMACOLOGY期刊下所有文献 > Reactive oxygen species are required for β2 adrenergic receptor-β-arrestin interactions and signaling to ERK1/2.

Reactive oxygen species are required for β2 adrenergic receptor-β-arrestin interactions and signaling to ERK1/2.

Abstract：

:The β2-adrenergic receptor (β2AR) is the prototypical member of the heptahelical G protein-coupled receptor (GPCR) superfamily and is well-known to elicit biological effects through both G protein-dependent and G protein-independent signaling cascades. Agonism of β2AR has been described to promote phosphorylation and activation of extracellular signal-regulated kinases (ERK1/2) via a G-protein/PKA pathway that transpires rapidly upon receptor agonism, as well as by a distinct β-arrestin-mediated pathway that occurs at later time points. We have previously shown that β2AR agonism promotes generation of intracellular reactive oxygen species (ROS) and that β2AR-associated G protein signaling is dependent on ROS formation. It has also been suggested that β2AR-mediated ROS generation occurs via recruitment of β-arrestins. In this study, we confirm the effects of β-arrestin on β2AR-induced ROS generation, and investigate the ROS-dependency of β-arrestin-linked β2AR signaling. In HEK293 cells, both coimmunoprecipitation and BRET studies reveal that ROS are vital for the physical interaction of β2AR with β-arrestin partner proteins. Using phosphorylation of ERK1/2 as a functional endpoint to assess the role of ROS in β2AR-β-arrestin signaling, our results show that inhibition of intracellular ROS abrogates both the β-arrestin and G protein-mediated phosphorylation of ERK1/2 upon agonism of β2AR. Importantly, both the G protein and β-arrestin components were reversed upon exogenous administration of ROS, suggesting a critical role for oxidants in stabilization of β2AR. Taken together, our data signify that ROS serve purposeful roles in stabilizing both G protein- and β-arrestin-mediated β2AR signaling in HEK293 cells.

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Singh M,Moniri NH

doi

10.1016/j.bcp.2012.06.012

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

661-9

issue

5

eissn

0006-2952

issn

1873-2968

pii

S0006-2952(12)00410-8

journal_volume

84

pub_type

杂志文章

相关文献

BIOCHEMICAL PHARMACOLOGY文献大全
  • Effects of metyrapone on microsomal-dependent Salmonella mutagenesis. Studies with chloroallyl ethers and model compounds.

    abstract：:Metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone, MTP) is used as an inhibitor of cytochrome P-450 enzymes, particularly those induced by phenobarbital (PB). We examined the effects of MTP on the microsomal dependent mutagenesis of a newly isolated promutagen, 3-(2-chloroethoxy)-1,2-dichloropropene (CP), three S-chlo...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(83)90144-2

    authors： Distlerath LM,Loper JC,Tabor MW

    更新日期：1983-12-15 00:00:00

  • Characterization of potent and selective iodonium-class inhibitors of NADPH oxidases.

    abstract：:The NADPH oxidases (NOXs) play a recognized role in the development and progression of inflammation-associated disorders, as well as cancer. To date, several NOX inhibitors have been developed, through either high throughput screening or targeted disruption of NOX interaction partners, although only a few have reached...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2017.07.007

    authors： Lu J,Risbood P,Kane CT Jr,Hossain MT,Anderson L,Hill K,Monks A,Wu Y,Antony S,Juhasz A,Liu H,Jiang G,Harris E,Roy K,Meitzler JL,Konaté M,Doroshow JH

    更新日期：2017-11-01 00:00:00

  • The Ca2+/CaMKK2 axis mediates the telbivudine induced upregulation of creatine kinase: Implications for mechanism of antiviral nucleoside analogs' side effect.

    abstract：:Telbivudine (LdT), a widely prescribed anti-hepatitis B virus (HBV) drug for the treatment of chronic Hepatitis B (CHB), causes adverse reactions ranging from creatine kinase (CK) elevation to myopathy. The purpose of this study was to explore the mechanism(s) of LdT induced CK elevation. The effects of LdT on mitocho...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2017.10.005

    authors： Jianfei L,Min W,Chunlai M,Bicui C,Jiming Z,Bin W

    更新日期：2017-12-15 00:00:00

  • Levels and subcellular distributions of detoxifying enzymes in the ovarian corpus luteum of the pregnant and non-pregnant pig.

    abstract：:The levels and subcellular distribution of enzymes involved in defenses against reactive oxygen superoxide dismutase (SOD; E.C.1.15.1.1), glutathione peroxidase (GPX; E.C.1.11.1.9), catalase (CAT; E.C.1.11.1.6), and DT-diaphorase (DT; E.C.1.6.99.2) and of the conjugating enzymes glutathione transferase (GST; E.C.2.5.1...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(99)00185-9

    authors： Eliasson M,Boström M,DePierre JW

    更新日期：1999-10-15 00:00:00

  • Neratinib decreases pro-survival responses of [sorafenib + vorinostat] in pancreatic cancer.

    abstract：:The combination of the multi-kinase and chaperone inhibitor sorafenib and the histone deacetylase inhibitor vorinostat in pancreatic cancer patients has proven to be a safe and efficacious modality (NCT02349867). We determined the evolutionary mechanisms by with pancreatic tumors become resistant to [sorafenib + vorin...

    journal_title：Biochemical pharmacology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.bcp.2020.114067

    authors： Booth L,Poklepovic A,Dent P

    更新日期：2020-08-01 00:00:00

  • Autophagic cell death, polyploidy and senescence induced in breast tumor cells by the substituted pyrrole JG-03-14, a novel microtubule poison.

    abstract：:JG-03-14, a substituted pyrrole that inhibits microtubule polymerization, was screened against MCF-7 (p53 wild type), MDA-MB231 (p53 mutant), MCF-7/caspase 3 and MCF-7/ADR (multidrug resistant) breast tumor cell lines. Cell viability and growth inhibition were assessed by the crystal violet dye assay. Apoptosis was ev...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2007.07.003

    authors： Arthur CR,Gupton JT,Kellogg GE,Yeudall WA,Cabot MC,Newsham IF,Gewirtz DA

    更新日期：2007-10-01 00:00:00

  • Molecular targets for emerging anti-tumor therapies for neurofibromatosis type 1.

    abstract：:Neurofibromatosis type 1 (NF1) is the most common cancer predisposition syndrome. NF1 patients present with a constellation of clinical manifestations and have an increased risk of developing certain benign and malignant tumors. This disease results from mutation within the gene encoding neurofibromin, a GTPase activa...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/j.bcp.2006.04.010

    authors： Dilworth JT,Kraniak JM,Wojtkowiak JW,Gibbs RA,Borch RF,Tainsky MA,Reiners JJ Jr,Mattingly RR

    更新日期：2006-11-30 00:00:00

  • Peripheral nitric oxide signaling directly blocks inflammatory pain.

    abstract：:Pain is a classical sign of inflammation, and sensitization of primary sensory neurons (PSN) is the most important mediating mechanism. This mechanism involves direct action of inflammatory mediators such as prostaglandins and sympathetic amines. Pharmacologic control of inflammatory pain is based on two principal str...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/j.bcp.2020.113862

    authors： Gomes FIF,Cunha FQ,Cunha TM

    更新日期：2020-06-01 00:00:00

  • Altered gene expression in human leukemia K562 cells selected for resistance to etoposide.

    abstract：:Sublines of K562 human leukemia cells were selected for resistance (30- to 80-fold) to etoposide by continuous exposure to 0.5 microM VP-16. Two etoposide-resistant cell lines, K/VP.5 and K/VP.5-1, showed a 5-fold reduction in levels of topoisomerase II alpha protein compared with K562 cells. Northern analysis indicat...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(93)90643-b

    authors： Ritke MK,Yalowich JC

    更新日期：1993-12-03 00:00:00

  • Immunospecific targeting of liposomes to erythrocytes.

    abstract：:Immunoliposomes were made by covalently linking Fab' fragments (from rabbit antimouse erythrocyte IgG) to reverse-phase evaporation vesicles (REV) via maleimido-4-(p-phenylbutyrate) phosphatidylethanolamine (MPB-PE) as anchor molecule. These immunoliposomes were characterized in terms of size, charge, stability and an...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(88)90584-9

    authors： Peeters PA,Claessens CA,Eling WM,Crommelin DJ

    更新日期：1988-06-01 00:00:00

  • Drug-induced lipid peroxidation in mice--V. Ethane production and glutathione release in the isolated liver upon perfusion with acetaminophen.

    abstract：:Isolated liver from phenobarbital-induced male mice was perfused using infusions of cytochrome c pulses as quality control of the system. Livers spontaneously evolved 1.1 pmoles ethane g-1 liver min-1, exogenous pentane disappeared with 0.6 pmoles g-1 min-1. Infusion of 0.26 mmoles/l. FeCl2 led to immediate ethane pro...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(83)90112-0

    authors： Thelen M,Wendel A

    更新日期：1983-06-01 00:00:00

  • The rigid steroid 21-hydroxy-6,19-epoxyprogesterone (21OH-6,19OP) is a dissociated glucocorticoid receptor modulator potentially useful as a novel coadjuvant in breast cancer chemotherapy.

    abstract：:Glucocorticoids (GCs) are steroid hormones widely used as coadjuvants in the treatment of solid tumors due to their anti-inflammatory effects. However, evidence show that they also may induce chemotherapy resistance, probably through their capacity to inhibit apoptosis triggered by antineoplastic drugs. GCs exert thei...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2014.04.006

    authors： Orqueda AJ,Dansey MV,Español A,Veleiro AS,Bal de Kier Joffé E,Sales ME,Burton G,Pecci A

    更新日期：2014-06-15 00:00:00

  • Chloroacetaldehyde-induced hepatocyte cytotoxicity. Mechanisms for cytoprotection.

    abstract：:2-Chloroacetaldehyde (CAA)-induced cytotoxicity in isolated hepatocytes was enhanced markedly if hepatocyte alcohol or aldehyde dehydrogenase was inhibited prior to CAA addition. Hepatocyte GSH depletion, ATP depletion and lipid peroxidation by CAA were also enhanced markedly. Furthermore, CAA was about 10- and 70-fol...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(94)90374-3

    authors： Sood C,O'Brien PJ

    更新日期：1994-08-30 00:00:00

  • The molecular and enzyme kinetic basis for the diminished activity of the cytochrome P450 2D6.17 (CYP2D6.17) variant. Potential implications for CYP2D6 phenotyping studies and the clinical use of CYP2D6 substrate drugs in some African populations.

    abstract：:In this study, the basis for the diminished capacity of CYP2D6.17 to metabolise CYP2D6 substrate drugs and the possible implications this might have for CYP2D6 phenotyping studies and clinical use of substrate drugs were investigated in vitro. Enzyme kinetic analyses were performed with recombinant CYP2D6.1, CYP2D6.2,...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(02)01351-5

    authors： Bapiro TE,Hasler JA,Ridderström M,Masimirembwa CM

    更新日期：2002-11-01 00:00:00

  • Functional expression of intestinal dipeptide/beta-lactam antibiotic transporter in Xenopus laevis oocytes.

    abstract：:An intestinal active transport system specific to small peptides and peptide-like drugs such as beta-lactam antibiotics was functionally expressed in Xenopus laevis oocytes after microinjection of messenger RNA (mRNA) derived from rat intestinal mucosal cells. The transport activity was evaluated by measuring the upta...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(94)90358-1

    authors： Tamai I,Tomizawa N,Kadowaki A,Terasaki T,Nakayama K,Higashida H,Tsuji A

    更新日期：1994-08-30 00:00:00

  • Heme oxygenase-1 induction by rosiglitazone via PKCα/AMPKα/p38 MAPKα/SIRT1/PPARγ pathway suppresses lipopolysaccharide-mediated pulmonary inflammation.

    abstract：:HO-1 (heme oxygenase-1), an antioxidant enzyme, induced by rosiglitazone (PPAR ligands) can be a potential treatment of inflammation. However, the mechanisms of rosiglitazone-induced HO-1 expression in human pulmonary alveolar epithelial cells (HPAEpiCs) remain largely unknown. In this study, we found that upregulatio...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2017.12.024

    authors： Cho RL,Lin WN,Wang CY,Yang CC,Hsiao LD,Lin CC,Yang CM

    更新日期：2018-02-01 00:00:00

  • Pharmacology of polymorphic variants of the human 5-HT1A receptor.

    abstract：:The 5-HT1A receptor is a critical mediator of serotonergic (5-HT) function. We have identified 13 potential single nucleotide polymorphisms resulting in amino acid changes throughout the human 5-HT1A receptor. The pharmacological profiles of these 13 polymorphic variants were then characterized using a high-throughput...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2003.09.030

    authors： Del Tredici AL,Schiffer HH,Burstein ES,Lameh J,Mohell N,Hacksell U,Brann MR,Weiner DM

    更新日期：2004-02-01 00:00:00

  • Inhibition of hyaluronan export from human fibroblasts by inhibitors of multidrug resistance transporters.

    abstract：:In a previous report we described the export of hyaluronan from Streptococcus pyogenes by an ABC transporter. Extending these findings a sequence homology search against human proteins revealed a strong homology to the multidrug resistance transporter ABC-B (MDR-1) and ABC-C (MRP 5). Using several inhibitors directed ...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2004.06.017

    authors： Prehm P,Schumacher U

    更新日期：2004-10-01 00:00:00

  • Effects of tannic acid on 12-O-tetradecanoylphorbol-13-acetate-induced protein kinase C activation in NIH 3T3 cells.

    abstract：:Tannic acid (TA) is a naturally occurring phenol, which has been found to display an antipromotion effect on mouse skin carcinogenesis. In order to explore the molecular mechanism, we have examined the process of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced protein kinase C (PKC) activation, including phorbol es...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(93)90095-e

    authors： Kuo ML,Wu WS,Lee KC,Lin JK

    更新日期：1993-10-19 00:00:00

  • The phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 modulates cytokine expression in macrophages via p50 nuclear factor κB inhibition, in a PI3K-independent mechanism.

    abstract：:The Phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002 (LY2), has been previously reported to inhibit nuclear factor κB (NFκB) activity, in a PI3K-independent mechanism. The goals of the current research were to determine the specificity of LY2 regarding NFκB subunits, and to identify relevant modulation of cyto...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2011.09.025

    authors： Avni D,Glucksam Y,Zor T

    更新日期：2012-01-01 00:00:00

  • The effects of inhibiting choline dehydrogenase on choline metabolism in mice.

    abstract：:3,3-Dimethylbutanol (Dimbunol), a competitive inhibitor of choline dehydrogenase (CDH), and ethylcholine mustard aziridinium (ECMA), an effective irreversible inhibitor of both CDH and choline transport, were investigated for their effects upon the uptake and metabolism of [3H]choline in mice. Thirty minutes after Dim...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(85)90156-x

    authors： Barlow P,Marchbanks RM

    更新日期：1985-09-01 00:00:00

  • Role of the endocannabinoid system in drug addiction.

    abstract：:Drug addiction is a chronic relapsing disorder that produces a dramaticglobal health burden worldwide. Not effective treatment of drug addiction is currently available probably due to the difficulties to find an appropriate target to manage this complex disease raising the needs for further identification of novel the...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/j.bcp.2018.09.013

    authors： Manzanares J,Cabañero D,Puente N,García-Gutiérrez MS,Grandes P,Maldonado R

    更新日期：2018-11-01 00:00:00

  • Structure-activity relationships for inhibition of human 5alpha-reductases by polyphenols.

    abstract：:The enzyme steroid 5 alpha-reductase (EC 1.3.99.5) catalyzes the NADPH-dependent reduction of the double bond of a variety of 3-oxo-Delta(4) steroids including the conversion of testosterone to 5 alpha-dihydrotestosterone. In humans, 5 alpha-reductase activity is critical for certain aspects of male sexual differentia...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(02)00848-1

    authors： Hiipakka RA,Zhang HZ,Dai W,Dai Q,Liao S

    更新日期：2002-03-15 00:00:00

  • Electron paramagnetic resonance spectrometry evidence for bioreduction of tirapazamine to oxidising free radicals under anaerobic conditions.

    abstract：:Tirapazamine (SR 4233) is a bioreductive antitumour drug in Phase III clinical trial which is activated in hypoxic tumour regions to generate a cytotoxic species. Electron paramagnetic resonance (EPR) spectrometry was used to investigate directly the formation of free radicals as the result of tirapazamine reduction b...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(00)00487-1

    authors： Patterson LH,Taiwo FA

    更新日期：2000-12-15 00:00:00

  • The inhibition of TNF-alpha-induced NF-kappaB activation by marine natural products.

    abstract：:The deregulated activation of NF-kappaB is associated with cancer development and inflammatory diseases. With an aim to find new NF-kappaB inhibitors, we purified and characterized compounds from extracts of the Fijian sponge Rhabdastrella globostellata, the crinoid Comanthus parvicirrus, the soft corals Sarcophyton s...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2009.05.009

    authors： Folmer F,Jaspars M,Solano G,Cristofanon S,Henry E,Tabudravu J,Black K,Green DH,Küpper FC,Aalbersberg W,Feussner K,Dicato M,Diederich M

    更新日期：2009-09-15 00:00:00

  • TPA-induced up-regulation of activator protein-1 can be inhibited or enhanced by analogs of the natural product curcumin.

    abstract：:The activator protein-1 (AP-1) family of transcription factors, including the most common member c-Jun-c-Fos, participates in regulation of expression of numerous genes involved in proliferation, apoptosis, and tumorigenesis in response to a wide array of stimuli including pro-inflammatory cytokines, growth factors, s...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2006.07.007

    authors： Weber WM,Hunsaker LA,Gonzales AM,Heynekamp JJ,Orlando RA,Deck LM,Vander Jagt DL

    更新日期：2006-10-16 00:00:00

  • Nobiletin improves hyperglycemia and insulin resistance in obese diabetic ob/ob mice.

    abstract：:Nobiletin is a polymethoxylated flavone found in certain citrus fruits that exhibits various pharmacological effects including anti-inflammatory, antitumor and neuroprotective properties. The present study investigated the effects of nobiletin on insulin sensitivity in obese diabetic ob/ob mice, and the possible mecha...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2010.01.034

    authors： Lee YS,Cha BY,Saito K,Yamakawa H,Choi SS,Yamaguchi K,Yonezawa T,Teruya T,Nagai K,Woo JT

    更新日期：2010-06-01 00:00:00

  • 13-cis retinoic acid and isomerisation in paediatric oncology--is changing shape the key to success?

    abstract：:Retinoic acid isomers have been used with some success as chemotherapeutic agents, most recently with 13-cis retinoic acid showing impressive clinical efficacy in the paediatric malignancy neuroblastoma. The aim of this commentary is to review the evidence that 13-cis retinoic acid is a pro-drug, and consider the impl...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章,评审

    doi：10.1016/j.bcp.2005.02.003

    authors： Armstrong JL,Redfern CP,Veal GJ

    更新日期：2005-05-01 00:00:00

  • Criteria for receptor sites in binding studies.

    abstract：:The study of binding is not an easy task especially because of the difficulty of interpreting the results in terms of binding on specific receptor sites. The problem is not new; what is new is the increasing amount of fanciful interpretation that such a technique has generated. The tendency to interpret anomalous or i...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(84)90436-2

    authors： Laduron PM

    更新日期：1984-03-15 00:00:00

  • Impaired thromboxane receptor dimerization reduces signaling efficiency: A potential mechanism for reduced platelet function in vivo.

    abstract：:Thromboxane A2 is a potent mediator of inflammation and platelet aggregation exerting its effects through the activation of a G protein-coupled receptor (GPCR), termed TP. Although the existence of dimers/oligomers in Class A GPCRs is widely accepted, their functional significance still remains controversial. Recently...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2016.11.010

    authors： Capra V,Mauri M,Guzzi F,Busnelli M,Accomazzo MR,Gaussem P,Nisar SP,Mundell SJ,Parenti M,Rovati GE

    更新日期：2017-01-15 00:00:00