Abstract:
:We studied the abnormal in vitro polyclonal B-cell activity observed in patients with multiple myeloma and Waldenström's macroglobulinaemia. Numbers of cells spontaneously secreting immunoglobulin (Ig) in freshly isolated suspensions of peripheral blood mononuclear cells and pokeweed mitogen (PWM) stimulated cultures of blood mononuclear cells were determined with a protein A reverse haemolytic plaque assay. These data were correlated with both the clinical and laboratory parameters of disease. Furthermore, Ig secreted into supernatants of PWM-stimulated cultures was examined by a light chain radioimmunoassay for evidence of in vitro activation of malignant B-cells. The mean level of circulating immunoglobulin secreting cells (IgSC) in patients was elevated when compared to that of normal subjects. The highest values were observed in those patients with the highest levels of serum paraprotein. However, experiments with cycloheximide suggested that such increased circulating IgSC values were often caused by the detection of Ig passively adsorbed to blood mononuclear cells. The studies with PWM stimulation of blood mononuclear cells were particularly revealing. Cultures of patient blood mononuclear cells with PWM showed depressed IgSC responses as a group compared to cultures of normal blood mononuclear cells; nevertheless, approximately half the patients demonstrated a sizeable response to PWM. No evidence for preferential activation of Ig secretion by the malignant B-cell clone was observed. Impaired PWM induced responses were associated with advanced or progressive clinical disease.
journal_name
Br J Haematoljournal_title
British journal of haematologyauthors
Levinson AI,Dziarski A,Blankenhorn E,Schreiber AD,Negendank WGdoi
10.1111/j.1365-2141.1985.tb07441.xsubject
Has Abstractpub_date
1985-07-01 00:00:00pages
437-48issue
3eissn
0007-1048issn
1365-2141journal_volume
60pub_type
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journal_title:British journal of haematology
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journal_title:British journal of haematology
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journal_title:British journal of haematology
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journal_title:British journal of haematology
pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-05-01 00:00:00
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pub_type: 杂志文章,实务指引,评审
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更新日期:2011-05-01 00:00:00
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更新日期:1997-02-01 00:00:00
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journal_title:British journal of haematology
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journal_title:British journal of haematology
pub_type: 杂志文章
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journal_title:British journal of haematology
pub_type: 杂志文章
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更新日期:1998-06-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章,评审
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更新日期:2007-12-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
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更新日期:1982-07-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
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更新日期:1985-02-01 00:00:00