Abstract:
:When using microelectrodes for intracellular ion activity studies, some uncertainties such as interference from cytosolic components at the microelectrode, cell damage, and cell contamination may arise. A model, which treats kinetic processes of the loss of carriers from the membrane phase of microelectrodes into the cytosol and cell membrane, is used for an estimation of the extent and time course of contamination by impaled ion-selective microelectrodes. An isolated model cell consisting of a plasma membrane surrounding a cytosolic milieu is assumed. The results of its considerations represent a worst case situation, in which significant contamination of the cell membrane of such a small isolated single cell might occur during time periods of electrophysiological experiments. In more complex situations, such as in intact tissues, the equilibrium membrane concentrations may be substantially less.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Oesch U,Ammann D,Simon Wdoi
10.1139/y87-142subject
Has Abstractpub_date
1987-05-01 00:00:00pages
885-8issue
5eissn
0008-4212issn
1205-7541journal_volume
65pub_type
杂志文章abstract::A significant relationship was observed between myocardial oxygen extraction and the extravascular mean time transit time. This relationship implies that oxygen extraction by the myocardium is a time-dependent process, and emphasizes the critical link between blood flow and myocardial oxygen consumption. ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y77-068
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y80-215
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y77-175
更新日期:1977-12-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y83-033
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y08-039
更新日期:2008-05-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y91-069
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y77-017
更新日期:1977-02-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y75-020
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journal_title:Canadian journal of physiology and pharmacology
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doi:10.1139/y90-168
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journal_title:Canadian journal of physiology and pharmacology
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doi:10.1139/y76-038
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y84-248
更新日期:1984-12-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y88-104
更新日期:1988-05-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
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更新日期:1992-01-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y88-058
更新日期:1988-04-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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