Abstract:
:Temporary block of glycolysis by 2-deoxy-D-glucose (2-DG) reversibly suppresses synaptic transmission in the CA1 region of hippocampal slices. Recovery of responses is followed by a sustained potentiation of field excitatory postsynaptic potentials (EPSPs) (2-DG-LTP). To investigate the mechanisms involved in this type of LTP, we studied the effects of 2-DG on membrane properties of CA1 neurons (in slices from Sprague-Dawley rats), recorded with sharp intracellular electrodes containing 3 M KCl, as well as patch electrodes, filled mainly with 150 mM KMeSO4 and Hepes. The predominant change produced by 15- to 20-min applications of 2-DG (10 mM, replacing glucose) was hyperpolarization (-5.6 +/- 1.1 mV for 18 intracellular recordings and -7.2 +/- 0.80 mV for 17 whole-cell recordings) accompanied by a fall in resistance (-33 +/- 2.5% for 14 intracellular recordings and -11.6 +/- 7.1% for 15 whole-cell recordings). Virtually identical hyperpolarizations were recorded in the presence of 20 microM glyburide (-5.5 +/- 1.5 mV, n = 6), but they were abolished by adenosine antagonists 8-(p-sulfophenyl)theophylline (8-SPT) and 8-cyclopentyl-3,7-dihydro-1,3-dipropyl-1H-purine-2,6-dione (DPCPX) (2.8 +/- 1.6 and 4.0 +/- 1.7 mV, respectively; n = 5 for both). It was concluded that the hyperpolarization is most likely caused by an increase in K+ conductance, activated by a 2-DG-induced rise in adenosine release. After such applications of 2-DG, a sustained potentiation of EPSPs (similar to the 2-DG-LTP of field EPSPs) was evident in five neurons recorded with intracellular electrodes but not in any of nine whole-cell recordings, where it was replaced by sustained, LTD-like depression. We conclude that a factor essential for 2-DG-LTP induction is lost during whole-cell recording.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Zhao YT,Tekkök S,Krnjević Ksubject
Has Abstractpub_date
1997-05-01 00:00:00pages
368-74issue
5eissn
0008-4212issn
1205-7541journal_volume
75pub_type
杂志文章abstract::Our objective was to investigate the effects of chronic excess iodine intake on thyroid functions and thyroid oxidative stress state in hypothyroid rats. Sixty rats were divided into euthyroid and hypothyroid (thiocyanate-induced) groups with or without administration of excess iodine (3000 or 6000 μg/L) for 8 weeks. ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y2012-046
更新日期:2012-05-01 00:00:00
abstract::The epicardial coronary venous pressure in 16 dogs was compared with coronary arterial pressure as well as aortic, intraventricular, and intramyocardial pressures. Partial aortic occlusion augmented intraventricular (IVP), intramyocardial (IMP), aortic (AP), and coronary arterial pressures. Peripheral coronary venous ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y83-033
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abstract::Slow alpha2-globulin (salpha2G), a high molecular weight glycoprotein, appears as a component of rat serum in a wide variety of both physiologic and pathologic conditions, including pregnancy; it is also present in the neonate. In the present study, the protein was detectable in serum after 6 to 10 days, and was prese...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y77-071
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y82-226
更新日期:1982-12-01 00:00:00
abstract::Several lines of evidence suggest that the responsiveness of the airways is heterogeneous, although the magnitude of this heterogeneity has not been quantified. We have developed a videomicroscopic method that allows the measurements of the responsiveness of individual explanted airways to contractile agonists such as...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Significant tension development resulting from the administration of lanthanum salts (La3+) to isolated aorta tissues was observed in preparations from the spontaneously hypertensive rat (SHR) but not in preparation from age-matched normotensive Wistar rats (NWR). The response to La3+ was significantly greater in tiss...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y77-148
更新日期:1977-10-01 00:00:00
abstract::Input to the nucleus medianus of the preoptic region has been suggested to be involved in both the drinking and pressor responses elicited by the central administration of angiotensin II. Evidence in support of this suggestion has been gained principally from electrical lesion experiments. This lesion procedure does n...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y88-176
更新日期:1988-08-01 00:00:00
abstract::Connexin43 has been recognized as forming gap junctions in Leydig cells. However, previous work has shown that mouse Leydig cells lacking this connexin do not suffer any limitation of their ability to produce testosterone when stimulated with luteinizing hormone. The objective of this study was to identify additional ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-2012-0385
更新日期:2013-02-01 00:00:00
abstract::A brief summary is given of some of the key points of the individual contributions at this International Brain Research Organization satellite meeting, presenting up-to-date, expert knowledge of energy metabolism, ionic turnover, and cell swelling at the cellular level of the mammalian central nervous system. On the b...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:10.1139/y92-250
更新日期:1992-01-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y08-039
更新日期:2008-05-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y86-215
更新日期:1986-10-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 临床试验,杂志文章
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更新日期:1995-01-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:10.1139/y05-117
更新日期:2006-03-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:10.1139/y94-084
更新日期:1994-05-01 00:00:00
abstract::This study investigated the glucose response to insulin infused after hepatic denervation. Hepatic denervations were performed on 13 anesthetized cats at different levels: (i) surgical hepatic anterior plexus denervation, (ii) chemical total hepatic denervation by painting phenol on the tissues around the portal vein,...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y93-024
更新日期:1993-02-01 00:00:00
abstract::The role of sympathetic innervation in the regulation of hindlimb skeletal muscle blood flow (QL) and metabolism was studied prior to and during acute anemia in anesthetized, paralyzed, and ventilated dogs (n = 8). Neural activity in the sciatic nerve was reversibly cold blocked for a 15-min period at control hematocr...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y88-189
更新日期:1988-09-01 00:00:00
abstract::The effects of some components of ischemia on the oscillatory (Vos) and nonoscillatory (V(ex)) potentials and respective currents (Ios and Iex), as well as their mechanisms, were studied in guinea pig isolated ventricular myocytes by means of a single-microelectrode, discontinuous voltage clamp method. Repetitive acti...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y94-056
更新日期:1994-04-01 00:00:00
abstract::To elucidate the involvement of the brain renin-angiotensin system and the brain atrial natriuretic polypeptide (ANP) system in the regulation of ANP secretion from the heart, the effects of intracerebroventricular administration of angiotensin II and ANP on the plasma ANP level were examined in conscious unrestrained...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y88-044
更新日期:1988-03-01 00:00:00
abstract::In addition to bronchial smooth muscle, histamine and other mediators act in bronchial asthma on the microcirculation of pulmonary connective tissue. The mediators induce enhanced blood flow, and by acting on the blood-tissue barrier, they induce increase in vasopermeability with edema and in more severe injury microh...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:10.1139/y87-077
更新日期:1987-03-01 00:00:00
abstract::Mentoring in academia is often carried out in an informal way depending on individuals and circumstances. I was quite fortunate to make the acquaintance of Professor E.E. Daniel when I was making a transition from my research in entomology to biomedical sciences. Here I recount some of that experience, and describe so...
journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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doi:10.1139/cjpp-2016-0439
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abstract::The purpose of the present investigation was to determine the nature of the functional interaction of muscarinic agonists with cAMP-generating and cAMP-independent agonists in left atria. Negative inotropic responses of rabbit isolated left atrial strips to the muscarinic agonist carbachol were measured in the absence...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y86-099
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abstract::The turnover rate of glucose, the irreversible disposal rate of lactate, and the rate of gluconeogenesis from lactate were calculated by tracer methods in four normal and four alloxan-diabetic dogs under control conditions as well as in chronic, stable metabolic acidosis and alkalosis. Acidosis was produced by feeding...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y88-025
更新日期:1988-02-01 00:00:00
abstract::The mammalian daily (circadian) clock is located in the suprachiasmatic nuclei of the hypothalamus. Clock function can be detected by the measurement of the circadian change in spontaneous firing rate of suprachiasmatic nuclei cells in a brain slice preparation in vitro. We investigated the effects of neuropeptide Y o...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::The validity of a Hodgkin-Huxley type voltage-dependent inactivation of slow inward Ca current (Isi) was tested in frog heart using a computer simulation. The time course of Isi was calculated during the development of a frog atrial action potential (AP). With a time constant of inactivation (tauf) of 55 ms at a membr...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y82-172
更新日期:1982-09-01 00:00:00
abstract::Cetamolol, a new beta-adrenoceptor blocker with partial agonist activity and cardioselectivity, was studied in vivo to determine its membrane-stabilizing effects. Comparisons were carried out with atenolol, pindolol, practolol, propranolol, timolol, dexpropranolol, lidocaine, and procaine. The following results indica...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y84-047
更新日期:1984-03-01 00:00:00
abstract::The threshold for electrically induced ventricular fibrillation was determined in anesthetized open-chest dogs, before and after blockade of atrioventricular (A-V) conduction. Without exception, the threshold was significantly elevated after creation of the block. The precise mechanism of this effect is not known at t...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y83-160
更新日期:1983-09-01 00:00:00
abstract::Sodium deoxycholate (NaDOC) inhibits the intestinal Ca2+ absorption and ursodeoxycholic acid (UDCA) stimulates it. The aim of this study was to determine whether NaDOC and UDCA produce differential effects on the redox state of duodenal mitochondria altering the Krebs cycle and the electron transport chain (ETC) funct...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-2019-0561
更新日期:2020-07-20 00:00:00
abstract::To assess development changes in kinetic properties of the cardiac sodium current, whole-cell voltage-clamp experiments were conducted using 3-, 10-, and 17-day-old embryonic chick ventricular heart cells. Experimental data were quantified according to the Hodgkin-Huxley model. While the Na current density, as examine...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y95-205
更新日期:1995-10-01 00:00:00
abstract::The development of cardiac hypertrophy was examined in rats given ethanol in a nutritionally adequate, liquid diet mixture, by intubation, in severely intoxicating doses at 8-h intervals for up to 96 h, alone or in combination with prazosin. Other groups of rats received isocalorically paired quantities of maltose-dex...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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