Real-time quantitative expression studies of the zearalenone biosynthetic gene cluster in Fusarium graminearum.

Abstract:

:The estrogenic mycotoxin zearalenone (ZON) produced by some Fusarium spp. causes reproductive problems and hyperestrogenic syndromes in mammals. In an effort to elucidate the molecular pathways of ZON production, we present a comparative real-time quantitative polymerase chain reaction expression study of seven contiguous genes in the ZON biosynthetic cluster on sterile rice and during wheat and oat infection. Under ZON production on rice, the polyketide synthase (PKS) genes PKS4 and PKS13, alcohol oxidase FG12056 gene, and transcriptional regulator FG02398 gene showed similarly upregulated patterns, whereas the nonribosomal peptide synthetase (NPS) FG02394, the K(+) channel beta subunit FG12015, and the protein kinase FG02399 displayed a variant pattern. During the same time period under wheat infection when no ZON was produced, the PKS genes and the NPS were downregulated relative to rice whereas the K(+) channel beta subunit gene FG12015 was markedly upregulated, suggesting that it may play a role in the infection process. This is the first expression study of ZON biosynthetic genes in planta. The results give insight into the regulation and activities of the ZON gene cluster under different experimental systems and suggest a connection between ZON and a K(+) channel that could reveal a novel function for ZON in Fusarium spp.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Lysøe E,Bone KR,Klemsdal SS

doi

10.1094/PHYTO-99-2-0176

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

176-84

issue

2

eissn

0031-949X

issn

1943-7684

pii

10.1094/PHYTO-99-2-0176

journal_volume

99

pub_type

杂志文章
  • Effect of Timing of Application of the Biological Control Agent Microsphaeropsis ochracea on the Production and Ejection Pattern of Ascospores by Venturia inaequalis.

    abstract::ABSTRACT Field and in vitro trials were conducted to establish the influence of the biological control agent Microsphaeropsis ochracea on the ejection pattern of ascospores by Venturia inaequalis and on apple scab development, and to establish the best timing of application. The ejection pattern of ascospores was simi...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.12.1305

    authors: Carisse O,Rolland D

    更新日期:2004-12-01 00:00:00

  • Particular Structure of Plasmopara viticola Populations Evolved Under Greek Island Conditions.

    abstract::ABSTRACT Plasmopara viticola populations collected from three islands in the Ionian Sea-an arm of the Mediterranean Sea to the west of Greece-were analyzed with microsatellite molecular markers in order to investigate the pathogen population structure. Downy mildew populations from mainland regions previously studied ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-0501

    authors: Rumbou A,Gessler C

    更新日期:2006-05-01 00:00:00

  • Characterizing the mechanism of biological control of postharvest diseases on fruits with a simple method to study competition for nutrients.

    abstract::ABSTRACT Biocontrol agents may compete with pathogens for nutrients and space to delay or prevent decay of fruits after harvest. These mechanisms of biological control have been difficult to study because no method has been available to determine the significance of each of the components of competition. We developed ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2000.90.11.1196

    authors: Janisiewicz WJ,Tworkoski TJ,Sharer C

    更新日期:2000-11-01 00:00:00

  • Meta-analysis of regression coefficients for the relationship between fusarium head blight and deoxynivalenol content of wheat.

    abstract::ABSTRACT A total of 126 field studies reporting deoxynivalenol (DON; ppm) content of harvested wheat grain and Fusarium head blight index (IND; field or plot-level disease severity) were analyzed to determine the overall mean regression slope and intercept for the relationship between DON and IND, and the influence of...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-0951

    authors: Paul PA,Lipps PE,Madden LV

    更新日期:2006-09-01 00:00:00

  • Effect of temperature, vector life stage, and plant access period on transmission of banana bunchy top virus to banana.

    abstract::The study of the transmission biology of insect-borne plant viruses is important to develop disease control practices. We characterized the transmission of a nanovirus, Banana bunchy top virus (BBTV), by its aphid vector Pentalonia nigronervosa Coquerel (Hemiptera, Aphididae) with respect to temperature, vector life s...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-98-6-0743

    authors: Anhalt MD,Almeida RP

    更新日期:2008-06-01 00:00:00

  • Resurgence of Less-Studied Smut Fungi as Models of Phytopathogenesis in the Omics Age.

    abstract::The smut fungi form a large, diverse, and nonmonophyletic group of plant pathogens that have long served as both important pests of human agriculture and, also, as fertile organisms of scientific investigation. As modern techniques of molecular genetic analysis became available, many previously studied species that pr...

    journal_title:Phytopathology

    pub_type: 杂志文章,评审

    doi:10.1094/PHYTO-02-16-0075-RVW

    authors: Toh SS,Perlin MH

    更新日期:2016-11-01 00:00:00

  • Sequence and simple-sequence repeat analyses of the fungal pathogen Seiridium cardinale indicate California is the most likely source of the cypress canker epidemic for the Mediterranean region.

    abstract::Seiridium cardinale is the pathogenic fungus of unknown origin responsible for a world pandemic known as cypress canker affecting several species of Cupressaceae in both the Northern and Southern Hemisphere. In this study, a comparative genetic analysis of worldwide populations was performed using sequence analysis of...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-05-11-0144

    authors: Della Rocca G,Eyre CA,Danti R,Garbelotto M

    更新日期:2011-12-01 00:00:00

  • A Promoter from Pea Gene DRR206 Is Suitable to Regulate an Elicitor-Coding Gene and Develop Disease Resistance.

    abstract::ABSTRACT Plant nonhost disease resistance is characterized by the induction of multiple defense genes. The pea DRR206 gene is induced following inoculation with pathogens and treatment with abiotic agents, and moderately induced by wounding. A deletion series of DRR206 promoter segments was fused with the beta-glucuro...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.6.651

    authors: Choi JJ,Klosterman SJ,Hadwiger LA

    更新日期:2004-06-01 00:00:00

  • Use of Green Fluorescent Protein and Image Analysis to Quantify Proliferation of Trichoderma harzianum in Nonsterile Soil.

    abstract::ABSTRACT One drawback of traditional methods for fungal biomass measurement is the inability to distinguish biomass of an introduced fungus from that of the indigenous microbial community in nonsterile soil. We quantified biomass of a specific fungal biological control agent in nonsterile soil using epifluorescence mi...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.12.1383

    authors: Orr KA,Knudsen GR

    更新日期:2004-12-01 00:00:00

  • The Use of Green Fluorescent Protein-Tagged Recombinant Viruses to Test Lettuce mosaic virus Resistance in Lettuce.

    abstract::ABSTRACT Seed certification and the use of cultivars containing one of two, probably allelic, recessive genes, mo1(1) and mo1(2), are the principal control methods for Lettuce mosaic virus (LMV) in lettuce. Although for a few LMV isolates, mo1(2) confers resistance with most isolates, the genes mo1(1) or mo1(2) confer...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2002.92.2.169

    authors: Candresse T,Le Gall O,Maisonneuve B,German-Retana S,Redondo E

    更新日期:2002-02-01 00:00:00

  • Effects of milsana and benzothiadiazole on the ultrastructure of powdery mildew haustoria on cucumber.

    abstract::ABSTRACT Disease assessments and cytological investigations provided valuable information on the modes of action and efficacies of two prophylactic compounds, Milsana and benzothiadiazole (BTH), against powdery mildew development on long English cucumber. Milsana application significantly reduced disease incidence rel...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.9.728

    authors: Wurms K,Labbé C,Benhamou N,Bélanger RR

    更新日期:1999-09-01 00:00:00

  • Genetic analysis of the resistance to eight anthracnose races in the common bean differential cultivar Kaboon.

    abstract::Resistance to the eight races (3, 7, 19, 31, 81, 449, 453, and 1545) of the pathogenic fungus Colletotrichum lindemuthianum (anthracnose) was evaluated in F(3) families derived from the cross between the anthracnose differential bean cultivars Kaboon and Michelite. Molecular marker analyses were carried out in the F(2...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-11-10-0296

    authors: Campa A,Giraldez R,Ferreira JJ

    更新日期:2011-06-01 00:00:00

  • Identification of sugarcane striate mosaic-associated virus by partial characterization of its double-stranded RNA.

    abstract::ABSTRACT Sugarcane striate mosaic (ScSM)-affected sugarcane leaves contain a disease-associated 9-kilobase (kb) double-stranded RNA (dsRNA), usually together with 6- and 2.6-kb dsRNAs. The purified 9-kb dsRNA was amplified by the randomly primed polymerase chain reaction (PCR) and cloned. The nucleotide sequences of t...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.10.877

    authors: Choi YG,Croft BJ,Randles JW

    更新日期:1999-10-01 00:00:00

  • Soybean sudden death syndrome species diversity within north and South america revealed by multilocus genotyping.

    abstract::Sudden death syndrome (SDS) of soybean has become a serious constraint to the production of this crop in North and South America. Phenotypic and multilocus molecular phylogenetic analyses, as well as pathogenicity experiments, have demonstrated that four morphologically and phylogenetically distinct fusaria can induce...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-1-0058

    authors: O'Donnell K,Sink S,Scandiani MM,Luque A,Colletto A,Biasoli M,Lenzi L,Salas G,González V,Ploper LD,Formento N,Pioli RN,Aoki T,Yang XB,Sarver BA

    更新日期:2010-01-01 00:00:00

  • Specific Diagnosis of Two Root-Knot Nematodes, Meloidogyne chitwoodi and M. fallax, with Satellite DNA Probes.

    abstract::ABSTRACT Meloidogyne chitwoodi and M. fallax are serious pests of potato, and both species have been recently designated as quarantine organisms in the European Community and in Canada. The sympatric and less damaging species M. hapla is often found associated with both of them under temperate climates. Here, we descr...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.5.380

    authors: Castagnone-Sereno P,Leroy F,Bongiovanni M,Zijlstra C,Abad P

    更新日期:1999-05-01 00:00:00

  • Host range, biological variation, and phylogenetic diversity of Eutypa lata in California.

    abstract::The objectives of this study were to investigate the host range of Eutypa lata in the major grape-growing regions in California and to analyze the phenotypic variation and phylogenetic diversity of E. lata isolates. Perithecia of E. lata were found on grapevines, in apricot, almond, cherry, apple, and pear tree orchar...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-02-10-0040

    authors: Trouillas FP,Gubler WD

    更新日期:2010-10-01 00:00:00

  • Factors Affecting the Release of Ascospores of Anisogramma anomala.

    abstract::ABSTRACT Relationships between environmental factors and release of ascospores of Anisogramma anomala, the causal agent of eastern filbert blight, were examined in four European hazelnut (Corylus avellana) orchards during a 2-year period. In each orchard, Burkhard volumetric spore traps and automated weather-monitorin...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1998.88.2.122

    authors: Pinkerton JN,Johnson KB,Stone JK,Ivors KL

    更新日期:1998-02-01 00:00:00

  • A methodology to detect and quantify five pathogens causing potato tuber decay using real-time quantitative polymerase chain reaction.

    abstract::ABSTRACT Late blight (Phytophthora infestans), pink rot (Phytophthora erythroseptica), leak (Pythium ultimum), dry rot (Fusarium sambucinum), and soft rot (Erwinia carotovora subsp. carotovora and subsp. atroseptica) are particularly damaging diseases of stored potato tubers worldwide. In this study, we present a meth...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-1037

    authors: Atallah ZK,Stevenson WR

    更新日期:2006-09-01 00:00:00

  • Polyphasic characterization of Xanthomonas axonopodis pv. allii associated with outbreaks of bacterial blight on three Allium species in the Mascarene archipelago.

    abstract::Based on the number of new reports during the last two decades, bacterial blight of onion (Allium cepa) is considered an emerging disease. The causal agent, Xanthomonas axonopodis pv. allii, is pathogenic to several Allium species after inoculation, but outbreaks worldwide have been primarily reported on onion. We des...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-98-8-0919

    authors: Picard Y,Roumagnac P,Legrand D,Humeau L,Robène-Soustrade I,Chiroleu F,Gagnevin L,Pruvost O

    更新日期:2008-08-01 00:00:00

  • Gentian mosaic virus: A New Species in the Genus Fabavirus.

    abstract::ABSTRACT A viral isolate, designated N-1 and obtained from a gentian (Gentiana scabra) plant that exhibited mosaic symptoms, was transmitted mechanically to nine plant species in six families. These plants are known as hosts of fabaviruses. The N-1 isolate was composed of isometric particles 30 nm in diameter and incl...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-0192

    authors: Kobayashi YO,Kobayashi A,Hagiwara K,Uga H,Mikoshiba Y,Naito T,Honda Y,Omura T

    更新日期:2005-02-01 00:00:00

  • Draft Whole Genome Sequence Analyses on Pseudomonas syringae pv. actinidiae Hypersensitive Response Negative Strains Detected from Kiwifruit Bleeding Sap Samples.

    abstract::Kiwifruit bleeding sap samples, collected in Italian and Chilean orchards from symptomatic and asymptomatic plants, were evaluated for the presence of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker. The saps were sampled during the spring in both hemispheres, before the bud sprouting, during...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-08-17-0278-R

    authors: Biondi E,Zamorano A,Vega E,Ardizzi S,Sitta D,De Salvador FR,Campos-Vargas R,Meneses C,Perez S,Bertaccini A,Fiore N

    更新日期:2018-05-01 00:00:00

  • Effect of Microclimate on Leveillula taurica Powdery Mildew of Sweet Pepper.

    abstract::ABSTRACT Sweet pepper-Leveillula taurica microclimate relations were studied under controlled conditions and in commercial greenhouses. Conidial germination occurred at 10 to 37 degrees C and was optimal at 20 degrees C. Conidial viability declined as temperatures increased to 40 degrees C for 6 h. Leaf colonization w...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-7-0813

    authors: Elad Y,Messika Y,Brand M,David DR,Sztejnberg A

    更新日期:2007-07-01 00:00:00

  • Disease-weather relationships for powdery mildew and yellow rust on winter wheat.

    abstract::Key weather factors determining the occurrence and severity of powdery mildew and yellow rust epidemics on winter wheat were identified. Empirical models were formulated to qualitatively predict a damaging epidemic (>5% severity) and quantitatively predict the disease severity given a damaging epidemic occurred. The d...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-98-5-0609

    authors: Te Beest DE,Paveley ND,Shaw MW,van den Bosch F

    更新日期:2008-05-01 00:00:00

  • Assessing changes and associations in the Xanthomonas perforans population across Florida commercial tomato fields via a state-wide survey.

    abstract::Before 1991, Xanthomonas euvesicatoria (Xe) was the causal agent of bacterial spot of tomato in Florida but was quickly replaced by Xanthomonas perforans (Xp). The Xp population has changed in genotype and phenotype despite lack of a clear selection pressure. To determine the current Xanthomonas population in Florida,...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-09-20-0402-R

    authors: Klein-Gordon J,Xing Y,Garrett KA,Abrahamian P,Paret ML,Minsavage GV,Strayer-Scherer AL,Fulton J,Timilsina S,Jones JB,Goss EM,Vallad GE

    更新日期:2020-10-13 00:00:00

  • Spiroplasma citri Spiralin Acts In Vitro as a Lectin Binding to Glycoproteins from Its Insect Vector Circulifer haematoceps.

    abstract::ABSTRACT In order to understand the molecular mechanisms underlying transmission of Spiroplasma citri by the leafhopper Circulifer haematoceps, we screened leafhopper proteins as putative S. citri-binding molecules using a spiroplasma overlay assay of protein blots (Far-western assay). Insect proteins were separated b...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-0541

    authors: Killiny N,Castroviejo M,Saillard C

    更新日期:2005-05-01 00:00:00

  • Genetic and Morphological Diversity of Temperate and Tropical Isolates of Phytophthora capsici.

    abstract::ABSTRACT Phytophthora capsici is a diverse species causing disease on a broad range of both temperate and tropical plants. In this study, we used cultural characteristics, amplified fragment length polymorphism (AFLP), and DNA sequence analyses of the ribosomal internal transcribed spacer (ITS) region and mitochondria...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-4-0492

    authors: Bowers JH,Martin FN,Tooley PW,Luz ED

    更新日期:2007-04-01 00:00:00

  • Inhibitory effects of antioxidant reagent in reactive oxygen species generation and penetration of appressoria of Alternaria alternata Japanese pear pathotype.

    abstract::In the Japanese pear pathotype of Alternaria alternata, H2O2 is generated solely from penetration pegs and not from other portions of subcuticular hyphae within the pectin layers of host leaves. A close association between H2O2 generation and fungal aggressiveness is expected because the pegs are important for fungal ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-9-0840

    authors: Hyon GS,Ikeda K,Hosogi N,Shinogi T,Park P

    更新日期:2010-09-01 00:00:00

  • Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana.

    abstract::The ability of four plant growth-promoting rhizobacteria, isolated in a previous study, to induce systemic resistance on Arabidopsis thaliana Col 0 against biotic and abiotic stress was evaluated. All the bacteria enhanced protection against the foliar pathogen Pseudomonas syringae DC3000 and increased plant tolerance...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-98-6-0666

    authors: Barriuso J,Solano BR,Gutiérrez Mañero FJ

    更新日期:2008-06-01 00:00:00

  • Ultrastructural and Cytochemical Investigation of Asymptomatic Infection by Pythium spp.

    abstract::ABSTRACT The influence exerted by Pythium group F (a minor pathogen ubiquitous in soilless cultures) and P. uncinulatum (a nonpathogenic species) colonization on tomato roots was investigated. In both interactions, infected roots did not exhibit obvious symptoms; however, major physiological changes occurred within th...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1998.88.3.234

    authors: Rey P,Benhamou N,Tirilly Y

    更新日期:1998-03-01 00:00:00

  • A Pectate Lyase Homolog, xagP, in Xanthomonas axonopodis pv. glycines Is Associated with Hypersensitive Response Induction on Tobacco.

    abstract::ABSTRACT Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule disease of soybeans. A transposon insertional mutant (KU-P-M670) of X. axonopodis pv. glycines derived from wild-type strain KU-P-34017 lost the ability to induce the hypersensitive response (HR) on tobacco and pepper but retained it...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-1230

    authors: Kaewnum S,Prathuangwong S,Burr TJ

    更新日期:2006-11-01 00:00:00