Effect of temperature, vector life stage, and plant access period on transmission of banana bunchy top virus to banana.

Abstract:

:The study of the transmission biology of insect-borne plant viruses is important to develop disease control practices. We characterized the transmission of a nanovirus, Banana bunchy top virus (BBTV), by its aphid vector Pentalonia nigronervosa Coquerel (Hemiptera, Aphididae) with respect to temperature, vector life stage, and plant access time. Adult aphids transmitted BBTV more efficiently than third instar nymphs at all temperatures tested. Adult aphids transmitted the virus more efficiently at 25 and 30 degrees C than at 20 degrees C, but temperature had no impact on transmission efficiency by nymphs. By decoupling the relationship between temperature and aphid BBTV acquisition or inoculation, we determined that temperature affected inoculation events more strongly than acquisition. Longer plant access periods increased viral acquisition and inoculation efficiencies in a range of 60 min to 24 h. Both BBTV acquisition and inoculation efficiencies peaked after 18 h of plant access period. We also show that BBTV transmission by P. nigronervosa requires a latent period. Our results demonstrate that vector transmission of BBTV is affected by temperature, vector life stage, and plant access period.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Anhalt MD,Almeida RP

doi

10.1094/PHYTO-98-6-0743

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

743-8

issue

6

eissn

0031-949X

issn

1943-7684

journal_volume

98

pub_type

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