Abstract:
:Kiwifruit bleeding sap samples, collected in Italian and Chilean orchards from symptomatic and asymptomatic plants, were evaluated for the presence of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker. The saps were sampled during the spring in both hemispheres, before the bud sprouting, during the optimal time window for the collection of an adequate volume of sample for the early detection of the pathogen, preliminarily by molecular assays, and then through its direct isolation and identification. The results of molecular analyses showed more effectiveness in the P. syringae pv. actinidiae detection when compared with those of microbiological analyses through the pathogen isolation on the nutritive and semiselective media selected. The bleeding sap analyses allowed the isolation and identification of two hypersensitive response (HR) negative and hypovirulent P. syringae pv. actinidiae strains from different regions in Italy. Moreover, multilocus sequence analysis (MLSA) and whole genome sequence (WGS) were carried out on selected Italian and Chilean P. syringae pv. actinidiae virulent strains to verify the presence of genetic variability compared with the HR negative strains and to compare the variability of selected gene clusters between strains isolated in both countries. All the strains showed the lack of argK and coronatine gene clusters as reported for the biovar 3 P. syringae pv. actinidiae strains. Despite the biologic differences obtained in the tobacco bioassays and in pathogenicity assays, the MLSA and WGS analyses did not show significant differences between the WGS of the HR negative and HR positive strains; the difference, on the other hand, between PAC_ICE sequences of Italian and Chilean P. syringae pv. actinidiae strains was confirmed. The inability of the hypovirulent strains IPV-BO 8893 and IPV-BO 9286 to provoke HR in tobacco and the low virulence shown in this host could not be associated with mutations or recombinations in T3SS island.
journal_name
Phytopathologyjournal_title
Phytopathologyauthors
Biondi E,Zamorano A,Vega E,Ardizzi S,Sitta D,De Salvador FR,Campos-Vargas R,Meneses C,Perez S,Bertaccini A,Fiore Ndoi
10.1094/PHYTO-08-17-0278-Rsubject
Has Abstractpub_date
2018-05-01 00:00:00pages
552-560issue
5eissn
0031-949Xissn
1943-7684journal_volume
108pub_type
杂志文章相关文献
PHYTOPATHOLOGY文献大全abstract::The study of the transmission biology of insect-borne plant viruses is important to develop disease control practices. We characterized the transmission of a nanovirus, Banana bunchy top virus (BBTV), by its aphid vector Pentalonia nigronervosa Coquerel (Hemiptera, Aphididae) with respect to temperature, vector life s...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-98-6-0743
更新日期:2008-06-01 00:00:00
abstract::ABSTRACT Ageratum conyzoides plants exhibiting yellow vein symptoms, collected near Haikou, Hainan Province, China, contained begomoviral DNA-A-like molecules. The complete sequences of the molecules from two samples, Hn2 and Hn2-19, were shown to consist of 2,768 and 2,748 nucelotides (nt), respectively. These sequen...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-97-4-0405
更新日期:2007-04-01 00:00:00
abstract::ABSTRACT Laboratory studies were conducted on the characteristics of beet yellows closterovirus (BYV) transmission to sugar beets by the bean aphid, Aphis fabae. The relative transmission efficiency and the retention, acquisition, and inoculation thresholds were evaluated using clonal aphid colonies. Transmission effi...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.7.766
更新日期:1997-07-01 00:00:00
abstract::ABSTRACT Genes for copper resistance, located on the chromosome of strain XvP26 of Xanthomonas axonopodis pv. vesicatoria, were transferred by conjugation to a recipient strain of the bacterium. The chromosomal gene transfer was verified by analyses of the genomes of donor, recipient, and putative transconjugants for ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1999.89.11.1044
更新日期:1999-11-01 00:00:00
abstract::Effects of the root-knot nematode Meloidogyne incognita and the fungal pathogen Thielaviopsis basicola on cotton seedling growth and root morphology were evaluated in controlled environmental experiments. Four pathogen treatments, including noninfested soil, soil infested with M. incognita, soil infested with T. basic...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-06-12-0120-R
更新日期:2014-05-01 00:00:00
abstract::ABSTRACT Biocontrol agents may compete with pathogens for nutrients and space to delay or prevent decay of fruits after harvest. These mechanisms of biological control have been difficult to study because no method has been available to determine the significance of each of the components of competition. We developed ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2000.90.11.1196
更新日期:2000-11-01 00:00:00
abstract::ABSTRACT The population structure of Guignardia citricarpa sensu lato (anamorph: Phyllosticta citricarpa), a fungus of which strains pathogenic to citrus are subject to phytosanitary legislation in the European Union and the United States, was investigated. Internal transcribed spacer sequences revealed two phylogenet...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2002.92.5.464
更新日期:2002-05-01 00:00:00
abstract::Studies on infection processes and gene expression were done to determine differential responses of cultivars of Trifolium subterraneum resistant and susceptible to infection by races of Phytophthora clandestina. In the infection process study, one race was inoculated onto the roots of T. subterraneum cvs. Woogenellup...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-100-6-0551
更新日期:2010-06-01 00:00:00
abstract::Xanthomonas oryzae pv. oryzicola is an important bacterial pathogen responsible for outbreaks of bacterial leaf streak (BLS) on rice, mostly occurring in Asia and parts of Africa. To better monitor epidemics and assess population structures, efficient tools that allow the precise identification and diagnosis of pathog...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-12-0078-R
更新日期:2012-10-01 00:00:00
abstract::Citrus canker, caused by Xanthomonas citri ssp. citri, is a serious disease that causes substantial economic losses to the citrus industry worldwide. The bactericide bismerthiazol has been used to control rice bacterial blight (X. oryzae pv. oryzae). In this paper, we demonstrate that bismerthiazol can effectively con...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-12-15-0328-R
更新日期:2016-07-01 00:00:00
abstract::Sudden death syndrome (SDS) of soybean has become a serious constraint to the production of this crop in North and South America. Phenotypic and multilocus molecular phylogenetic analyses, as well as pathogenicity experiments, have demonstrated that four morphologically and phylogenetically distinct fusaria can induce...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-100-1-0058
更新日期:2010-01-01 00:00:00
abstract::Bois noir phytoplasma (BNp), widespread in wine-producing areas of Europe and endemic in France and Italy, is classified in the 16SrXII-A subgroup, whose members are referred to as Stolbur phytoplasmas. The 16S rDNA gene of Stolbur phytoplasma shows low variability, and few non-ribosomal genes are available as markers...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-99-6-0711
更新日期:2009-06-01 00:00:00
abstract::Vitis rupestris B38 is a North American grapevine resistant to the major pathogen of cultivated grapevines, Erysiphe necator. Sources of powdery mildew resistance, like V. rotundifolia, are widely used in grape breeding but are already threatened, even before commercialization, by isolates that can reproduce on Run1 a...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-09-14-0260-R
更新日期:2015-08-01 00:00:00
abstract::Exploring the genetic diversity and evolutionary history of plant viruses is critical to understanding their ecology and epidemiology. In this study, maximum-likelihood and population genetics-based methods were used to investigate the population structure, genetic diversity, and sources of genetic variation in field ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-01-10-0035
更新日期:2011-01-01 00:00:00
abstract::ABSTRACT Monoconidial isolates of Venturia inaequalis were collected in 1990 and 1991 from orchards in New York, Michigan, and Nova Scotia that had never or only sporadically been treated with fungicides acting as sterol demethylation inhibitors (DMIs). Sensitivities of isolates to two representative DMIs (fenarimol a...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.2.184
更新日期:1997-02-01 00:00:00
abstract::Huanglongbing (HLB) or greening currently is the most devastating citrus disease worldwide. The fastidious phloem-colonizing bacterium 'Candidatus Liberibacter asiaticus' (CLas) is the causal agent of citrus HLB in Florida. Bactericides containing the active ingredient oxytetracycline (OTC) have been used in foliar sp...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-06-19-0198-R
更新日期:2019-12-01 00:00:00
abstract::ABSTRACT A collection of race 1 and race 2 isolates of Fusarium oxysporum f. sp. lycopersici was screened for vegetative compatibility and characterized by random amplified polymorphic DNA (RAPD) analysis to establish the identity and genetic diversity of the isolates. Comparison of RAPD profiles revealed two main gro...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1999.89.2.156
更新日期:1999-02-01 00:00:00
abstract::Pythium species are important soilborne pathogens occurring in the forest nursery industry of the Pacific Northwest. However, little is known about their genetic diversity or population structure and it is suspected that isolates are moved among forest nurseries on seedling stock and shared field equipment. In order t...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-05-14-0147-R
更新日期:2015-05-01 00:00:00
abstract::Oomycetes are fungal-like pathogens that cause notorious diseases. Protecting crops against oomycetes requires regular spraying with chemicals, many with an unknown mode of action. In the 1990s, flumorph was identified as a novel crop protection agent. It was shown to inhibit the growth of oomycete pathogens including...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-14-0119-R
更新日期:2015-04-01 00:00:00
abstract::ABSTRACT Fusarium oxysporum f. sp. ciceris and the root-lesion nematode Pratylenchus thornei coinfect chickpeas in southern Spain. The influence of root infection by P. thornei on the reaction of Fusarium wilt-susceptible (CPS 1 and PV 61) and wilt-resistant (UC 27) chickpea cultivars to F. oxysporum f. sp. ciceris ra...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.8.828
更新日期:1998-08-01 00:00:00
abstract::Wine contamination with ochratoxin A (OTA) is due to the attack of wine grapes by ochratoxigenic Aspergillus carbonarius and Aspergillus spp. section Nigri. Four A. pullulans strains, AU14-3-1, AU18-3B, AU34-2, and LS30, are resistant to and actively degrade ochratoxin A in vitro. The less toxic ochratoxin alpha and t...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-98-12-1261
更新日期:2008-12-01 00:00:00
abstract::ABSTRACT Spot blotch, caused by Cochliobolus sativus, is one of the most common foliar diseases of barley in the upper midwest region of the United States. To examine the genetics of host-specific virulence in C. sativus, a cross was made between isolate ND90Pr (which exhibits high virulence on barley genotype Bowman ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.11.1140
更新日期:1997-11-01 00:00:00
abstract::Sweetpotato production in the United States is limited by several postharvest diseases, and one of the most common is Fusarium root rot. Although Fusarium solani is believed to be the primary causal agent of disease, numerous other Fusarium spp. have been reported to infect sweetpotato. However, the diversity of Fusar...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-01-16-0009-R
更新日期:2016-08-01 00:00:00
abstract::Downy mildew of grape caused by Plasmopara viticola is a global pathogen of economic importance to commercial viticulture. In contrast to populations in the northern hemisphere, few studies have investigated the population biology, genetic diversity, and origin of the pathogen in Australian production systems. DNA was...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-19-0146-R
更新日期:2019-11-01 00:00:00
abstract::ABSTRACT It has been speculated that the N-terminal half of the readthrough domain (RTD) encoded by open reading frame 5 of Soybean dwarf virus (SbDV) is related to the vector specificity. To further investigate this hypothesis, transmissibility via aphids was tested on 17 SbDV isolates and comparisons of the deduced ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2003.93.12.1560
更新日期:2003-12-01 00:00:00
abstract::A study was designed to screen individual strains of plant growth-promoting rhizobacteria (PGPR) for broad-spectrum disease suppression in vitro and in planta. In a preliminary screen, 28 of 196 strains inhibited eight different tested pathogens in vitro. In a secondary screen, these 28 strains showed broad spectrum a...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-17-0051-R
更新日期:2017-08-01 00:00:00
abstract::ABSTRACT The effects of take-all epidemics on winter wheat yield formation were determined, and disease-yield relationships were established to assess the agronomic efficacy and economic benefits of control methods. Epidemics were generated in naturally infested fields by varying cropping season, crop order in the rot...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2001.91.7.694
更新日期:2001-07-01 00:00:00
abstract::ABSTRACT Severe epidemics of Fusarium head blight (FHB) caused by Fusarium graminearum, group II (teleomorph: Gibberella zeae) have been occurring on wheat crops in the northcentral United States and southern Canada. Evaluation of resistance to FHB is difficul, because resistance is partial and infection depends upon ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.10.1078
更新日期:1998-10-01 00:00:00
abstract::Pseudomonas syringae pv. syringae, the causal agent of bacterial apical necrosis (BAN) in mango crops, has been isolated in different mango-producing areas worldwide. An extensive collection of 87 P. syringae pv. syringae strains isolated from mango trees affected by BAN from different countries, but mainly from South...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-13-0093-R
更新日期:2013-11-01 00:00:00
abstract::ABSTRACT Our objective was to determine the value of corn (Zea mays) inbred Oh516 as a source of resistance to Aspergillus ear rot and aflatoxin accumulation in grain. Types and magnitudes of gene action associated with resistance were determined with generation means analysis. Molecular markers associated with resist...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2004.94.10.1107
更新日期:2004-10-01 00:00:00