Effects of milsana and benzothiadiazole on the ultrastructure of powdery mildew haustoria on cucumber.

Abstract:

:ABSTRACT Disease assessments and cytological investigations provided valuable information on the modes of action and efficacies of two prophylactic compounds, Milsana and benzothiadiazole (BTH), against powdery mildew development on long English cucumber. Milsana application significantly reduced disease incidence relative to inoculated controls through induction of localized resistance. Microscopic observations showed most haustoria had collapsed in the localized Milsana treatment and were encapsulated by an amorphous material impregnated by electron-opaque substances. The rapidity of haustorial collapse (within 4 days of treatment application) together with the encasement by electron-dense substances stained blue by toluidine blue O suggest that phenolics are possibly involved in the Milsana defense response. Cytochemical labeling of chitin with a wheat germ agglutinin/ovomucoid-gold complex showed that complete cellular disorganization of the fungus had occurred without disturbance to chitin in the walls of mycelia and haustoria. This may indicate that chitinolytic activity is not important in the Milsana-activated defense response. Application of high doses of BTH induced occasional cell wall thickening and accumulation of a compound that stained purple by toluidine blue O, but the defense response was weak, sporadic, and insufficient to reduce powdery mildew infection on cucumber. Responses to BTH could not be differentiated in terms of timing of the initial application or systemicity.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Wurms K,Labbé C,Benhamou N,Bélanger RR

doi

10.1094/PHYTO.1999.89.9.728

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

728-36

issue

9

eissn

0031-949X

issn

1943-7684

journal_volume

89

pub_type

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