Xanthomonas oryzae pv. oryzae XKK.12 contains an AroQgamma chorismate mutase that is involved in rice virulence.

Abstract:

:Chorismate mutase (CM) is a key enzyme in the shikimate pathway which is responsible for the synthesis of aromatic amino acids. There are two classes of CMs, AroQ and AroH, and several pathogenic bacteria have been reported to possess a subgroup of CMs designated AroQ(gamma). These CMs are usually exported to the periplasm or outside the cell; in a few cases, they have been reported to be involved in virulence and their precise role is currently unknown. Here, we report that the important rice pathogen Xanthomonas oryzae pv. oryzae XKK.12 produces an AroQ(gamma) CM which we have purified and characterized from spent supernatants. This enzyme is synthesized in planta and X. oryzae pv. oryzae knock-out mutants are hypervirulent to rice. The role of this enzyme in X. oryzae pv. oryzae rice virulence is discussed.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Degrassi G,Devescovi G,Bigirimana J,Venturi V

doi

10.1094/PHYTO-100-3-0262

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

262-70

issue

3

eissn

0031-949X

issn

1943-7684

journal_volume

100

pub_type

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