Abstract:
:Chlorotic streak is a global disease of commercial sugarcane (Saccharum spp. hybrids). The disease is transmitted by wet soil, water, as well as in diseased planting material. Although first recognized almost 90 years ago and despite significant research effort, the identity of the causal agent has been elusive. Metagenomic high throughput sequencing (HTS) facilitated the discovery of novel protistan ribosomal and nuclear genes in chlorotic streak-infected sugarcane. These sequences suggest a possible causal agent belonging to the order Cercomonadida (Rhizaria, phylum Cercozoa). An organism with morphological features similar to cercomonads (=Cercomonadida) was isolated into pure axenic culture from internal stalk tissues of infected sugarcane. The isolated organism contained DNA sequences identical to those identified in infected plants by HTS. The DNA sequences and the morphology of the organism did not match any known species. Here we present a new genus and species, Phytocercomonas venanatans, which is associated with chlorotic streak of sugarcane. Amplicon sequencing also supports that P. venanatans is associated with this disease. This is the first reported member from Cercomonadida showing a probable pathogenic association with higher plants.
journal_name
Phytopathologyjournal_title
Phytopathologyauthors
Ngo CN,Braithwaite KS,Bass D,Young AJ,Croft BJdoi
10.1094/PHYTO-07-17-0237-Rsubject
Has Abstractpub_date
2018-04-01 00:00:00pages
479-486issue
4eissn
0031-949Xissn
1943-7684journal_volume
108pub_type
杂志文章相关文献
PHYTOPATHOLOGY文献大全abstract::ABSTRACT Late leaf spot disease caused by Cercosporidium personatum is one of the most destructive foliar diseases of peanut (Arachis hypogaea) worldwide. The objective of this research was to identify resistance genes in response to leaf spot disease using microarray and real-time polymerase chain reaction (PCR). To ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-95-0381
更新日期:2005-04-01 00:00:00
abstract::ABSTRACT Double-stranded RNA (dsRNA) was purified from grapevines infected with grapevine leafroll-associated viruses 4 (GLRaV-4) and 5 (GLRaV-5), two putative closteroviruses. Reverse-transcriptase polymerase chain reaction (RT-PCR) was performed on this dsRNA using degenerate oligonucleotides designed to amplify an ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.11.1238
更新日期:1998-11-01 00:00:00
abstract::Based on the number of new reports during the last two decades, bacterial blight of onion (Allium cepa) is considered an emerging disease. The causal agent, Xanthomonas axonopodis pv. allii, is pathogenic to several Allium species after inoculation, but outbreaks worldwide have been primarily reported on onion. We des...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-98-8-0919
更新日期:2008-08-01 00:00:00
abstract::Phytophthora species are destructive fungus-like plant pathogens that use asexual single-celled flagellate zoospores for dispersal and plant infection. Many of the zoospore behaviors are density-dependent although the underlying mechanisms are poorly understood. Here, we use P. nicotianae as a model and demonstrate au...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-100-7-0632
更新日期:2010-07-01 00:00:00
abstract::The defense-related gene LsGRP1 exhibits an increased level of expression in Lilium spp. after being infected by Botrytis elliptica, the fungal pathogen of lily leaf blight. In this study, the expression profile of the LsGRP1 protein (a plant class II glycine-rich protein) was characterized biochemically and its subce...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-01-14-0020-R
更新日期:2014-10-01 00:00:00
abstract::ABSTRACT Inoculation of bean hypocotyls with a nonpathogenic binucleate Rhizoctonia (BNR) species induced systemic resistance and protection of the roots and cotyledons to later challenge with the root rot pathogen Rhizoctonia solani or the anthracnose pathogen Colletotrichum lindemuthianum. Bean seedlings that were t...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.4.359
更新日期:1998-04-01 00:00:00
abstract::Although 'Candidatus Liberibacter asiaticus' (Las) is a major pathogen associated with citrus huanglongbing (HLB), some characteristics of transmission by the psyllid vector Diaphorina citri are not fully understood. We examined the latent period and persistence of transmission of Las by D. citri in a series of experi...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-16-0088-R
更新日期:2017-03-01 00:00:00
abstract::ABSTRACT Alternative control agents, including UV-type C (254 nm) irradiation, yeasts antagonistic to fungal growth, chitosan and harpin, were evaluated for their ability to induce resistance in cv. Red Delicious apple fruit against postharvest blue mold caused by Penicillium expansum. Freshly harvested and controlled...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2002.92.8.900
更新日期:2002-08-01 00:00:00
abstract::The deposition of callose is a common plant defense response to intruding pathogens and part of the plant's innate immunity. In this study, eight grapevine callose synthase (CalS) genes were identified and characterized. To investigate biological function of CalS in grapevine against the infection of Plasmopara vitico...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-07-15-0166-R
更新日期:2016-01-01 00:00:00
abstract::The genetic variability of a Cucurbit aphid-borne yellows virus (CABYV) (genus Polerovirus, family Luteoviridae) population was evaluated by determining the nucleotide sequences of two genomic regions of CABYV isolates collected in open-field melon and squash crops during three consecutive years in Murcia (southeaster...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-11-12-0280-R
更新日期:2013-11-01 00:00:00
abstract::ABSTRACT A new foliar disease on coffee leaves was observed in Mindanao, Philippines, in 1996. The symptoms appeared as large circular or irregularly shaped necrotic areas with small circular necrotic spots (1 mm or less in diameter) usually found around the periphery of the large necrotic areas. Rhizoctonia solani wa...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2001.91.11.1054
更新日期:2001-11-01 00:00:00
abstract::Cercospora sojina causes frogeye leaf spot of soybean, which can cause serious economic losses in the United States. In this study, 132 C. sojina isolates were collected from six fields (from two counties, Cross and Crawford) in Arkansas. To determine mating type, a multiplex polymerase chain reaction assay was develo...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-09-12-0229-R
更新日期:2013-10-01 00:00:00
abstract::ABSTRACT A major leaf rust (Puccinia triticina) resistance quantitative trait locus (QTL) (QLrP.sfr-7DS) previously has been described on chromosome 7DS in the winter wheat (Triticum aestivum) cv. Forno. It was detected in a population of single-seed descent (SSD) lines derived from the cross Arina x Forno. QLrP.sfr-7...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2004.94.10.1036
更新日期:2004-10-01 00:00:00
abstract::Blast, caused by Pyricularia oryzae, has become an economically important disease in wheat in Brazil, but little effort has been devoted to understanding the wheat-P. oryzae interaction. This study was intended to determine the effects of P. oryzae infection on the photosynthetic process in wheat plants using a suscep...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-01-13-0024-R
更新日期:2014-01-01 00:00:00
abstract::Acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, has caused considerable damage to the watermelon and melon industry in China and the United States. Understanding the emergence and spread of this pathogen is important for controlling the disease. To build a fingerprinting database for reliabl...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-99-8-0913
更新日期:2009-08-01 00:00:00
abstract::ABSTRACT Three similar isolates of Phytophthora (Phytophthora sp-h) were obtained from diseased Spathiphyllum and Primula plants. Cultural characteristics did not fit any known description of Phytophthora species. The Phytophthora sp-h isolates are papillate, are homothallic, possess 80 to 86% amphigynous antheridia, ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.9.922
更新日期:1998-09-01 00:00:00
abstract::ABSTRACT Aflatoxins are carcinogens produced mainly by Aspergillus flavus during infection of susceptible crops such as maize. Through proteomic comparisons of maize kernel embryo proteins of resistant and susceptible genotypes, several protein spots previously were found to be unique or upregulated in resistant embry...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2004.94.9.938
更新日期:2004-09-01 00:00:00
abstract::ABSTRACT Phytophthora capsici is a diverse species causing disease on a broad range of both temperate and tropical plants. In this study, we used cultural characteristics, amplified fragment length polymorphism (AFLP), and DNA sequence analyses of the ribosomal internal transcribed spacer (ITS) region and mitochondria...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-97-4-0492
更新日期:2007-04-01 00:00:00
abstract::Venturia effusa (syn. Fusicladium effusum), causal agent of pecan scab, is the most prevalent pathogen of pecan (Carya illinoinensis), causing severe yield losses in the southeastern United States. V. effusa is currently known only by its asexual (conidial) stage. However, the degree and distribution of genetic divers...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-07-17-0233-R
更新日期:2018-07-01 00:00:00
abstract::The evolutionary relationships among Verticillium dahliae vegetative compatibility (VCG) subgroups VCG1A, VCG1B, VCG2A, VCG2B, VCG4A, VCG4B, and VCG6 were investigated by parsimony analysis of amplified fragment length polymorphism (AFLP) fingerprints and sequences of six DNA regions (actin, beta-tubulin, calmodulin, ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-98-9-1019
更新日期:2008-09-01 00:00:00
abstract::Rice blast is one of the most damaging diseases of rice worldwide. In the present study, we analyzed DNA sequence variation of avirulence (AVR) genes of AVR-Pita1 in field isolates of Magnaporthe oryzae in order to understand the effectiveness of the resistance gene Pi-ta in China. Genomic DNA of 366 isolates of M. or...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-11-13-0302-R
更新日期:2014-07-01 00:00:00
abstract::Mummy berry, caused by Monilinia vaccinii-corymbosi, causes economic losses of highbush blueberry in the U.S. Pacific Northwest (PNW). Apothecia develop from mummified berries overwintering on soil surfaces and produce ascospores that infect tissue emerging from floral and vegetative buds. Disease control currently re...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-17-0162-R
更新日期:2017-11-01 00:00:00
abstract::ABSTRACT Calla lily chlorotic spot virus (CCSV) isolated from central Taiwan was recently identified as a tospovirus serologically but distantly related to Watermelon silver mottle virus (WSMoV). To clarify the serological relationship between the two viruses, rabbit polyclonal antibody (PAb) to CCSV and mouse monoclo...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-95-1482
更新日期:2005-12-01 00:00:00
abstract::Boerhavia erecta plants in and around agricultural fields in the Azua Valley of the southeastern Dominican Republic often show striking golden mosaic symptoms. Leaf samples from B. erecta plants showing these symptoms were collected in 2012 and 2013, and PCR tests with degenerate primers revealed begomovirus DNA-A and...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-19-0061-R
更新日期:2019-08-01 00:00:00
abstract::Yellowing diseases of sugar beet can be caused by a range of strains classified as Beet mild yellowing virus (BMYV) or Beet western yellows virus (BWYV), both belonging to the genus Polerovirus of the family Luteoviridae. Host range, genomic, and serological studies have shown that isolates of these viruses can be gro...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2000.90.5.460
更新日期:2000-05-01 00:00:00
abstract::Curly top in sugar beet continues to be a challenging disease to control in the western United States. To aid in development of host resistance and management options, the curtovirus species composition was investigated by sampling 246 commercial fields along with nursery and field trials in the western United States....
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-98-11-1212
更新日期:2008-11-01 00:00:00
abstract::ABSTRACT A fungivorous nematode, Aphelenchoides sp., was isolated from field soil by baiting with mycelium of the biocontrol fungus Trichoderma harzianum ThzID1, and subsequently was maintained on agar cultures of the fungus. Interactions between the nematode and the green fluorescent protein-producing transformant, T...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2001.91.3.301
更新日期:2001-03-01 00:00:00
abstract::ABSTRACT Laboratory studies were conducted on the characteristics of beet yellows closterovirus (BYV) transmission to sugar beets by the bean aphid, Aphis fabae. The relative transmission efficiency and the retention, acquisition, and inoculation thresholds were evaluated using clonal aphid colonies. Transmission effi...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.7.766
更新日期:1997-07-01 00:00:00
abstract::Potyviral helper component protease (HC-Pro), as a major determinant of symptom expression in susceptible plants, is a likely target candidate in the production of attenuated strains for cross-protection. In this study, single or double mutations of Lys (K) to Glu (E) in the Lys-Ile-Thr-Cys motif and Arg (R) to Ile (I...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-09-19-0328-R
更新日期:2020-01-01 00:00:00
abstract::The new broad-spectrum fungicides from the succinate dehydrogenase inhibitor (SDHI) class have been quickly adopted by the market, which may lead to a high selection pressure on various pathogens. Cases of resistance have been observed in 14 fungal pathogens to date and are caused by different mutations in genes encod...
journal_title:Phytopathology
pub_type: 杂志文章,评审
doi:10.1094/PHYTO-01-13-0009-RVW
更新日期:2013-09-01 00:00:00