Abstract:
:ABSTRACT In order to understand the molecular mechanisms underlying transmission of Spiroplasma citri by the leafhopper Circulifer haematoceps, we screened leafhopper proteins as putative S. citri-binding molecules using a spiroplasma overlay assay of protein blots (Far-western assay). Insect proteins were separated by one- or two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, blotted, and probed with S. citri proteins. In this in vitro assay, we found that spiroplasma proteins exhibited affinity for seven leafhopper proteins. The interactions between S. citri proteins and insect proteins with molecular masses of 50 and 60 kDa were found to be sugar sensitive. These insect proteins were identified as high mannose N-glycoproteins, which support an interaction of glycoprotein-lectin type with S. citri proteins. Lectin detection in S. citri has revealed only one protein of 24 kDa. Using a leafhopper protein overlay assay on an S. citri protein blot, one spiroplasma protein with a similar molecular mass of 24 kDa was shown to display an insect protein-binding capacity. This protein was identified as the spiralin, which is the most abundant membrane protein of S. citri. Far-western experiments performed with purified spiralin and insect glycoproteins confirmed the binding of spiralin to the insect glycoproteins of 50 and 60 kDa. Thus, the spiralin could play a key role in the transmission of S. citri by mediating spiroplasma adherence to epithelial cells of insect vector gut or salivary gland.
journal_name
Phytopathologyjournal_title
Phytopathologyauthors
Killiny N,Castroviejo M,Saillard Cdoi
10.1094/PHYTO-95-0541subject
Has Abstractpub_date
2005-05-01 00:00:00pages
541-8issue
5eissn
0031-949Xissn
1943-7684journal_volume
95pub_type
杂志文章相关文献
PHYTOPATHOLOGY文献大全abstract::ABSTRACT The population structure of Guignardia citricarpa sensu lato (anamorph: Phyllosticta citricarpa), a fungus of which strains pathogenic to citrus are subject to phytosanitary legislation in the European Union and the United States, was investigated. Internal transcribed spacer sequences revealed two phylogenet...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2002.92.5.464
更新日期:2002-05-01 00:00:00
abstract::Nicotiana benthamiana is a valuable model organism in plant biology research. This report describes its extended applicability in the field of molecular plant pathology by introducing a nonbiotrophic fungal pathogen Cercospora nicotianae that can be conveniently used under laboratory conditions, consistently induces a...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-12-16-0448-R
更新日期:2018-01-01 00:00:00
abstract::ABSTRACT Barley has two flowering types, chasmogamous (open-flowering) and cleistogamous (closed-flowering). We examined the effect of the timing of Fusarium graminearum infection on Fusarium head blight (FHB) and mycotoxin accumulation in barley cultivars with different flowering types using greenhouse experiments. I...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-97-9-1054
更新日期:2007-09-01 00:00:00
abstract::ABSTRACT The recent development of molecular-based probes such as mono- and polyclonal antibodies, cloned phytoplasma DNA fragments, and phytoplasma-specific primers for polymerase chain reaction (PCR) has allowed for advances in detection and identification of uncultured phytoplasmas (formerly called mycoplasma-like ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.12.1359
更新日期:1998-12-01 00:00:00
abstract::Reniform nematode (RN) is an important pest in cotton production. Knowledge of the distribution patterns of RN is essential for selecting sampling strategies and for site-specific management. A 3-year study was conducted in two fields in South Carolina with the purpose of characterizing the distribution of RN using a ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-09-14-0240-R
更新日期:2015-05-01 00:00:00
abstract::ABSTRACT The species X. index, X. diversicaudatum, X. vuittenezi, and X. italiae are established (E) or putative (P) vectors of Grapevine fanleaf virus (GFLV) (E), Arabis mosaic virus (E), Grapevine chrome mosaic virus (P), and GFLV (P) nepoviruses of grapevine, respectively. All four species are very closely related ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2003.93.2.160
更新日期:2003-02-01 00:00:00
abstract::ABSTRACT Restriction fragment length polymorphisms (RFLPs) were used to characterize the genetic structures of three field populations of Phaeosphaeria nodorum from Texas, Oregon, and Switzerland. Data from seven nuclear RFLP loci were used to estimate gene diversity and genetic distances and to make indirect measures...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.3.353
更新日期:1997-03-01 00:00:00
abstract::ABSTRACT Leptosphaeria maculans causes blackleg of oilseed rape. Gene-for-gene interactions between race PG3 and Brassica napus cv. Quinta were related to interaction between the fungal avirulence (Avr) gene AvrLm1 and the corresponding resistance gene Rlm1. AvrLm1 isolates were aviru-lent on cvs. Doublol, Vivol, Colu...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2001.91.1.70
更新日期:2001-01-01 00:00:00
abstract::Zebra chip (ZC) of potato is putatively caused by the fastidious, phloem-limited bacterium 'Candidatus Liberibacter solanacearum' (Lso), which is transmitted by the potato psyllid (Bactericera cockerelli). The disease, which significantly impacts both crop yield and quality, was first identified in the United States f...
journal_title:Phytopathology
pub_type: 杂志文章,评审
doi:10.1094/PHYTO-12-14-0365-FI
更新日期:2015-07-01 00:00:00
abstract::Plants have developed complex defense mechanisms to protect themselves against pathogens. A wide-host-range fungus, Austropuccinia psidii, which has caused severe damage to ecosystems and plantations worldwide, is a major threat to Australian ecosystems dominated by members of the family Myrtaceae. In particular, the ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-09-17-0307-R
更新日期:2018-04-01 00:00:00
abstract::ABSTRACT Digestive cysteine proteinases have been isolated from plant-parasitic nematodes as well as coleopteran and hemipteran insects. Phytocystatins, inhibitors of cysteine proteinases, are found in a number of plants where they may play a role in defense against pathogens and pests. The cDNAs of the phytocystatins...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2003.93.7.799
更新日期:2003-07-01 00:00:00
abstract::ABSTRACT Our objective was to determine the value of corn (Zea mays) inbred Oh516 as a source of resistance to Aspergillus ear rot and aflatoxin accumulation in grain. Types and magnitudes of gene action associated with resistance were determined with generation means analysis. Molecular markers associated with resist...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2004.94.10.1107
更新日期:2004-10-01 00:00:00
abstract::ABSTRACT Isolates of Colletotrichum spp. from almond, avocado, and strawberry from Israel and isolates of the pink subpopulation from almond from the United States were characterized by various molecular methods and compared with morphological identification. Taxon-specific primer analysis grouped the avocado isolates...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2000.90.6.608
更新日期:2000-06-01 00:00:00
abstract::Lysobacter enzymogenes strain C3 (LeC3) is a potential biocontrol agent for plant diseases caused by fungi and oomycetes. Understanding the interaction between LeC3 and soybean pathogens at the molecular level could help improve its biocontrol efficacy. In this study, we obtained mutants with decreased abilities in in...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-09-19-0356-R
更新日期:2020-03-01 00:00:00
abstract::The role of Grapevine Pinot gris virus (GPGV) in the etiology of grapevine leaf mottling and deformation was investigated by biological and molecular assays. A survey on different cultivars from the Trentino Region in Italy showed a widespread distribution of GPGV, which was associated with symptomatic (79%) but also ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-09-14-0241-R
更新日期:2015-04-01 00:00:00
abstract::ABSTRACT The study was conducted to evaluate the potential of induced resistance to infestation of sunflower (Helianthus annuus L.) by the parasitic weed Orobanche cumana Wallr. Treatment of sunflower seeds with 40 ppm of benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH) for 36 h completely prevented inf...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2002.92.1.59
更新日期:2002-01-01 00:00:00
abstract::Raffaelea lauricola, a fungal symbiont of the ambrosia beetle Xyleborus glabratus, causes laurel wilt in members of the Lauraceae plant family. North American species in the family, such as avocado (Persea americana) and swamp bay (P. palustris), are particularly susceptible to laurel wilt, whereas the Asian camphortr...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-16-0072-R
更新日期:2017-01-01 00:00:00
abstract::The ability to develop fungicide resistance was assessed in Monilinia fructicola isolates with different fungicide sensitivity phenotypes by adapting mycelium and conidia to increasing concentrations of selective fungicides and UV mutagenesis. Results showed that adaptation to Quinone outside inhibitor (QoI) fungicide...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-98-2-0230
更新日期:2008-02-01 00:00:00
abstract::Triticum pathotype (MoT) of Magnaporthe oryzae (syn. Pyricularia oryzae) causes wheat blast, which has recently spread to Asia. To assess the potential risk of wheat blast in rice-wheat growing regions, we investigated the pathogenicity of 14 isolates of P. oryzae on 32 wheat cultivars, among which MoO isolates were c...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-10-20-0470-R
更新日期:2021-01-20 00:00:00
abstract::Pierce's disease (PD) of Vitis vinifera grapevines is caused by the bacterium Xylella fastidiosa, a pathogen with a wide plant host range. Exposure of X. fastidiosa-infected plant tissue to cold temperatures has been shown to be effective at eliminating the pathogen from some plant hosts such as grapevines. This "cold...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-08-10-0207
更新日期:2011-12-01 00:00:00
abstract::ABSTRACT Application of salicylic acid induces systemic acquired resistance in tobacco. pchA and pchB, which encode for the biosynthesis of salicylic acid in Pseudomonas aeruginosa, were cloned into two expression vectors, and these constructs were introduced into two root-colonizing strains of P. fluorescens. Introdu...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1998.88.7.678
更新日期:1998-07-01 00:00:00
abstract::ABSTRACT Fusarium fungi, including F. culmorum, cause seedling blight, foot rot, and head blight diseases of cereals, resulting in yield loss. In a screen for potential disease control organisms and agents, Pseudomonas fluorescens strains MKB 100 and MKB 249, P. frederiksbergensis strain 202, Pseudomonas sp. strain MK...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-96-0386
更新日期:2006-04-01 00:00:00
abstract::ABSTRACT Septoria speckled leaf blotch (SSLB) caused by Septoria passerinii is a common disease in barley. SSLB resistance genes Rsp1, Rsp2, and Rsp3 have previously been identified in the United States Department of Agriculture National Small Grains collection accessions CIho 14300, CIho 4780, and CIho 10644, respect...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-97-2-0155
更新日期:2007-02-01 00:00:00
abstract::ABSTRACT Crown rust (Puccinia coronata) in indigenous populations of Avena sterilis has been cited as an example of stability of wild pathosystems that consist of natural mixtures of resistance and virulence. This study confirmed that virulence/avirulence polymorphisms in P. coronata on A. sterilis in Israel are highl...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2004.94.5.505
更新日期:2004-05-01 00:00:00
abstract::Forty-eight inbred lines of maize with varying levels of resistance to gray leaf spot (GLS) were artificially inoculated with Cercospora zeina and evaluated to characterize partial disease resistance in maize under field conditions from 2012 to 2014 across 12 environments in western Kenya. Eight measures of disease ep...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-12-19-0446-R
更新日期:2020-10-01 00:00:00
abstract::ABSTRACT Meloidogyne chitwoodi and M. fallax are serious pests of potato, and both species have been recently designated as quarantine organisms in the European Community and in Canada. The sympatric and less damaging species M. hapla is often found associated with both of them under temperate climates. Here, we descr...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1999.89.5.380
更新日期:1999-05-01 00:00:00
abstract::Studies on infection processes and gene expression were done to determine differential responses of cultivars of Trifolium subterraneum resistant and susceptible to infection by races of Phytophthora clandestina. In the infection process study, one race was inoculated onto the roots of T. subterraneum cvs. Woogenellup...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-100-6-0551
更新日期:2010-06-01 00:00:00
abstract::ABSTRACT Phytophthora capsici is a diverse species causing disease on a broad range of both temperate and tropical plants. In this study, we used cultural characteristics, amplified fragment length polymorphism (AFLP), and DNA sequence analyses of the ribosomal internal transcribed spacer (ITS) region and mitochondria...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-97-4-0492
更新日期:2007-04-01 00:00:00
abstract::Few molecular details of effectors of Heterodera avenae parasitism are known. We performed a high-throughput sequencing analysis of the H. avenae transcriptome at five developmental stages. A total of 82,549 unigenes were ultimately obtained, and 747 transcripts showed best hits to genes putatively encoding carbohydra...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-07-17-0226-R
更新日期:2018-02-01 00:00:00
abstract::Monilinia fructicola is a fungal pathogen of worldwide significance that causes brown rot of stone fruits. There are only few reports related to the production of biologically active polyketides by this pathogen. In this study, we examined an atypical M. fructicola strain TW5-4 that shows strong antimicrobial activity...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-20-0059-R
更新日期:2020-08-01 00:00:00