Abstract：
:Diarrhoeagenic Escherichia coli (DEC), an important agent of infectious diarrhoea, is constantly evolving, making it necessary for its periodic monitoring. Unfortunately, DEC genotypes in Ghana remain uncharacterised. We focused on characterising the molecular serotypes, virulence factors, multilocus sequence types, and the phylogenetic relatedness among different DEC pathotypes recovered from stool samples of paediatric patients with diarrhoea symptoms from the Western region of Ghana. We detected all five common DEC pathotypes, with majority being enterotoxigenic E. coli (ETEC) isolates harbouring the heat-labile enterotoxin gene. The DEC strains exhibited a diverse serotypic identity with novel and other outbreak strains. Sequence type (ST)38, ST316, and ST1722 were the most prevalent STs, and clonal complex (CC)10 was the most common CC. A close evolutionary distance was observed among most of the isolates. Coli surface antigen 6 was the most prevalent (44%, n = 11) ETEC-specific colonisation factor. Nearly all of the isolates harboured lpfA, and the frequencies of other virulence genes, such as pap and cnf1, were 7.9% and 18.4%, respectively. This study provides insights into the important and novel genotypes circulating in the Western region of Ghana that should be monitored for public health.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Prah I,Ayibieke A,Huong NTT,Iguchi A,Mahazu S,Sato W,Hayashi T,Yamaoka S,Suzuki T,Iwanaga S,Ablordey A,Saito Rdoi
10.7883/yoken.JJID.2020.356subject
Has Abstractpub_date
2020-08-31 00:00:00eissn
1344-6304issn
1884-2836pub_type
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