Abstract:
:In the present study, we examined the prevalence of Providencia spp. strains among children with diarrhea in Japan. We developed a Providencia genus-specific polymerase chain reaction (PCR) method, and the specificity and sensitivity was evaluated to be 100% with various bacterial strains including 7 genera and 13 species. Five of 345 samples (1.4%) were positive by PCR using a Providencia genus-specific primer targeting the 16S rRNA gene. The single species Providencia rettgeri was isolated from 4 stool samples of children with diarrhea. The prevalence of Providencia spp. in children with diarrhea in Japan is lower than that previously reported for Japanese travelers abroad with diarrhea.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Shima A,Hinenoya A,Asakura M,Nagita A,Yamasaki Sdoi
10.7883/yoken.65.545subject
Has Abstractpub_date
2012-01-01 00:00:00pages
545-7issue
6eissn
1344-6304issn
1884-2836pii
DN/JST.JSTAGE/yoken/65.545journal_volume
65pub_type
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