Abstract:
:Argininosuccinate synthetase and argininosuccinate lyase catalyze the conversion of citrulline to arginine in kidney. Immunohistochemical staining of mouse kidney sections with antibodies to these two enzymes, compared with the staining patterns of known markers for proximal tubules, demonstrated that these enzymes are localized within the proximal tubules. The relative abundance of mRNA encoding argininosuccinate synthetase and argininosuccinate lyase during fetal and postnatal development of mouse kidney was also determined. Changes in relative abundance of these mRNA in kidney are coordinate during development, paralleling the developmental profile of phosphoenolpyruvate carboxykinase mRNA, which is also expressed in proximal tubules. Although relative abundances of the mRNA are comparable in liver and kidney of adult mice, the profiles of mRNA abundance during development of these two organs are distinct. The results indicate that these enzymes and their corresponding mRNA can serve as useful markers for examining the differentiation and development of renal proximal tubules in vivo and in cultured explants.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Morris SM Jr,Sweeney WE Jr,Kepka DM,O'Brien WE,Avner EDdoi
10.1203/00006450-199102000-00010subject
Has Abstractpub_date
1991-02-01 00:00:00pages
151-4issue
2eissn
0031-3998issn
1530-0447journal_volume
29pub_type
杂志文章abstract::Platelet-activating factor (PAF) is a proinflammatory phospholipid mediator implicated in necrotizing enterocolitis. Regulation of PAF-acetylhydrolase (AH), the enzyme degrading PAF, is poorly understood. In this study we found that administration of a dose of PAF (1.5 microg/kg, i.v.), which does not cause gross inte...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199711000-00008
更新日期:1997-11-01 00:00:00
abstract::Intrauterine growth restriction (IUGR) is a recognized risk factor for neurologic deficits later in life. Abnormal fetal blood flow in the presence of fetal growth retardation helps to distinguish true fetal growth impairment from small but normally grown infants. The present study aimed to investigate the influence o...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000129660.32875.09
更新日期:2004-07-01 00:00:00
abstract::Mucosal samples from rabbit jejunum were incubated (30 min, 25 degrees C) with (125I)glycinin in the presence of buffer, soybean lectin (50 micrograms/ml) soyasaponins (1 mg/ml), or both lectin and saponins. The mucosal uptake of (125I)glycinin was negligible with buffer, and increased progressively with additions of ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198209000-00005
更新日期:1982-09-01 00:00:00
abstract::Medium chain acyl-CoA dehydrogenase deficiency (MCAD) is a defect in the mitochondrial oxidation of fatty acids. The disorder typically presents with episodes of vomiting and hypoglycemia, sometimes with changes in mental status and hepatic failure. These Reye's-like features may culminate in coma and death. Stress, i...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199505000-00021
更新日期:1995-05-01 00:00:00
abstract::The isobutyryl-CoA dehydrogenase (IBD) enzyme is involved in the degradation of valine. IBD deficiency was first reported in 1998 and subsequent genetic investigations identified acyl-CoA dehydrogenase (ACAD) 8, now IBD, as the gene responsible for IBD deficiency. Only three individuals homozygous or compound heterozy...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000233085.72522.04
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:Secondhand smoke (SHS) exposure at home and fetal SHS exposure during pregnancy are a major cause of disease among children. The aim of this study is quantifying the burden of disease due to SHS exposure in children and in pregnancy in 2006-2017 for the 28 European Union (EU) countries. METHODS:Exposure to ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-020-01223-6
更新日期:2020-11-04 00:00:00
abstract::The fibroblast of the fetal and neonatal lung is intimately involved with lung development and function. Additionally, the perinatal rat lung fibroblast is a significant source of prostaglandins (PG) I2 and E2, which in turn affect lung development and function. Their effects may be mediated by cAMP. We, therefore, te...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199204000-00008
更新日期:1992-04-01 00:00:00
abstract::A double-label, double-substrate incubation technique has been developed and used to study the conversion of progesterone to testosterone in testes extracts from incompletely virilized males. The procedure involves separation of the microsomes from a testicular homogenate, incubating the microsomes with 1 microM [7-3H...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198408000-00018
更新日期:1984-08-01 00:00:00
abstract::To clarify the prevalence of Japanese children thought to be at a risk for infection with GB virus-C (GBV-C)/hepatitis G virus (HGV), we investigated the detection rates of serum GBV-C/ HGV ribonucleic acid (RNA) by reverse transcription-seminested PCR and serum anti-HGV-E2 antibody by ELISA in 162 children with histo...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1203/00006450-199901000-00021
更新日期:1999-01-01 00:00:00
abstract::The carbohydrate-deficient glycoprotein syndromes are a recently individualized group of genetic multisystemic disorders. A predominant feature is a severe involvement of the central and peripheral nervous system resulting in psychomotor retardation, seizures, ataxia, and, mostly after infancy, stroke-like episodes. T...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199305000-00024
更新日期:1993-05-01 00:00:00
abstract::Tyrosine (Tyr) phosphorylation is implicated in the modification of several erythrocyte functions, such as metabolic pathways and membrane transport, as well as in signal transduction systems. Here we describe the map of Tyr-phosphorylated soluble proteins of newborn red blood cells (RBC) using an in vitro model simul...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000180545.24457.AC
更新日期:2005-10-01 00:00:00
abstract::Antigenic analysis of neuroblastoma cells was performed by autologous typing and indirect immunofluorescent test combined with absorption tests. Serum of a patient with neuroblastoma before treatment showed weak reactivity with cultured autologous neuroblastoma cells, while serum obtained from the same patient 3 month...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198610000-00001
更新日期:1986-10-01 00:00:00
abstract::Respiratory parameters of skeletal muscle were determined in permeabilized muscle fibers by adapting a technique described by Veksler et al. for cardiac fibers (Biochim Biophys Acta, 892:191-196, 1987). This method consists of the permeabilization of muscle fibers by saponin by allowing respiratory substrates and inhi...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199207000-00004
更新日期:1992-07-01 00:00:00
abstract::BackgroundTo investigate the association between electronic cigarette (e-cig) use and smoking cessation among smokers who called the Youth Quitline in Hong Kong.MethodsThis longitudinal study collected data on youth smokers' (N=189) use and perception of e-cigs, conventional cigarette smoking behavior, and sociodemogr...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2017.80
更新日期:2017-07-01 00:00:00
abstract::GH insensitivity may be an inherited condition or may arise as a consequence of disease of malnutrition. Laron syndrome is the most severe form of GH insensitivity, arising from an absent or defective GH receptor. Less severe forms of GH insensitivity, however, may exist, resulting in short stature but in few other fe...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199605000-00018
更新日期:1996-05-01 00:00:00
abstract::We examined adult sheep lymphocytes for the following surface markers: Surface membrane immunoglobulin, antigen identified by anti-sheep thymocyte globulin and complement receptors. We quantitated sheep peripheral blood mononuclear leukocyte proliferative responses to mitogens and alloantigens, and demonstrated a neut...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198405000-00012
更新日期:1984-05-01 00:00:00
abstract::Studies were conducted to assess the efficacy and safety of a synthetic peptide-containing surfactant in the treatment of respiratory distress syndrome (RDS) in preterm (approximately 80% of normal gestation) infant rhesus monkeys. Surfactant was prepared consisting of the phospholipids dipalmitoylphosphatidyl choline...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199604000-00025
更新日期:1996-04-01 00:00:00
abstract::Pyritinol-HCl was tested for its impact on the cognitive functions of children with learning disabilities. This study is a contribution to scientific discussion on the complicated methodologic problems in evaluating the clinical efficacy of psychopharmacologic agents. Sixty-seven pupils of slow learner classes between...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1203/00006450-197509000-00006
更新日期:1975-09-01 00:00:00
abstract::The primary manifestations of Pompe disease are muscle weakness and cardiomyopathy. Although accumulation of glycogen has also been seen in the nervous system in patients, the significance of brain involvement in infantile-onset Pompe disease is not clear. In this study, brain development in five cases of infantile-on...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000233014.84318.4e
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:The aim of this study was to determine whether small-for-gestational-age (SGA) infants born very prematurely had increased respiratory morbidity in the neonatal period and at follow-up. METHODS:Data were examined from infants recruited into the United Kingdom Oscillation Study (UKOS). Of the 797 infants who...
journal_title:Pediatric research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/pr.2012.201
更新日期:2013-04-01 00:00:00
abstract::To investigate the role of nutrition in the regulation of IGFs during the perinatal period, 10-d-old rats were infused intravenously with various concentrations of nutrients for 24 h. Breast-fed litter mates served as controls. The effect of caloric intake on concentrations of IGF-I and IGF-II as well as IGF-binding p...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198908000-00012
更新日期:1989-08-01 00:00:00
abstract::Few data exist regarding resuscitation of hypovolemic shock in infants, and alternative strategies such as vasopressor therapy merit further evaluation. However, the effects of norepinephrine on cerebral perfusion and oxygenation during hemorrhagic shock in the pediatric population are still unclear. Eight anesthetize...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181425858
更新日期:2007-10-01 00:00:00
abstract::We studied the effect of maternal concentration and mode of administration on transfer rate of TSH-releasing hormone (TRH) in an in vitro model of dually perfused isolated lobule of human term placenta. Varying concentrations (4-40 microg dL(-1)) of TRH were added either to the open maternal circulation to mimic an in...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199705000-00012
更新日期:1997-05-01 00:00:00
abstract::Neonatal group B streptococcal (GBS) sepsis produces pulmonary arterial hypertension and hypoxemia that are preventable by pretreatment with the selective thromboxane A2 synthase inhibitor, dazmegrel. In the present experiment we administered dazmegrel (8 mg/kg) 2 h after the initiation of a 2 1/2 h infusion of 5 X 10...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198804000-00003
更新日期:1988-04-01 00:00:00
abstract::The present study employed an animal model in an attempt to document effects on brain growth of long-term exposure to theophylline in the presence or absence of malnutrition. One group of dams was randomly divided into half. One-half was fed a 20% protein diet and the other half a 6% protein diet. Similarly, a second ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198406000-00014
更新日期:1984-06-01 00:00:00
abstract::Chitosan is a natural, cationic polysaccharide derived from fully or partially deacetylated chitin. Chitosan is capable of inducing large phospholipid aggregates, closely resembling the function of nonionic polymers tested previously as additives to therapeutic lung surfactants. The effects of chitosan on improving th...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000227558.14024.57
更新日期:2006-08-01 00:00:00
abstract::The effect of ingestion of uncooked cornstarch (2 g/kg body weight) in water, uncooked starch (1 g/kg) added to a meal, and glucose (2 g/kg) in water, was studied in eight patients with type IA glycogen storage disease (GSD) and one patient with type IB GSD. Blood glucose concentrations were determined at 30-min inter...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198409000-00015
更新日期:1984-09-01 00:00:00
abstract::To determine the role of pili in mediating adherence of type b and nontypeable Haemophilus influenzae to several respiratory cell types, piliated bacteria, nonpiliated phase variants, and nonpiliated mutants possessing insertionally inactivated pilin genes were tested. Both piliated and nonpiliated strains adhered to ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199602000-00025
更新日期:1996-02-01 00:00:00
abstract::We measured body composition, basal metabolic rate (BMR), and total energy expenditure in 28 nonobese and 35 obese adolescents aged 12-18 y using indirect calorimetry and the doubly labeled water method. BMR was highly correlated with fat-free mass in both the nonobese and obese groups (r = 0.77 and 0.84, respectively...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199002000-00022
更新日期:1990-02-01 00:00:00
abstract::This patient presented on the first day of life with pronounced lactic acidosis with an elevated lactate/pyruvate ratio. Urine organic acids showed Krebs cycle metabolites and mildly elevated methylmalonate and methylcitrate. The acylcarnitine profile showed elevated propionylcarnitine and succinylcarnitine. Amino aci...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181e5c3a4
更新日期:2010-08-01 00:00:00