Abstract:
:Carbapenemase-mediated carbapenem resistance is a major public health concerns worldwide. In the present study, prevalence of circulating carbapenemases was estimated among carbapenem-resistant clinical isolates using PCR and sequencing. Diameters of zone of inhibition (ZDs) were compared for imipenem, meropenem and ertapenem among single carbapenemase producing isolates. Structure-based functional fitness of those carbapenemases was predicted through several in silico analyses. Approximately, 63.76% isolates demonstrated carbapenem resistance, of which 39.13% harboured carbapenemases like blaNDM-1 (33.23%), blaNDM-1-like (0.31%), blaVIM-2 (4.35%), blaKPC-2 (4.04%), blaOXA-181 (6.85%), blaOXA-23 (16.50%), blaOXA-69 (3.88%), blaOXA-66 (2.91%) and blaOXA-104 (1.94%). Omega values indicated selection pressure over blaOXA-69, blaOXA-66 and blaOXA-104. Protein structural dynamics predicted NDM-1 and KPC-2 to have the highest and least flexibility, indicating differences in β-lactam binding and catalytic efficiency. Increased requirement of free folding energy, improved solvent accessibility and decreased melting temperatures among NDM-1-like, OXA-181, OXA-66, OXA-69 and OXA-104 predicted functional improvement over their ancestral variants. NDM-1-like carbapenemases demonstrated improvement in binding stability, affinity and catalysis of meropenem than that of NDM-1. Catalytic activity of imipenem was predicted to improve among OXA-181, which could be correlated with more than 1.5 folds smaller ZDs around imipenem disc, than that of meropenem/ertapenem, among OXA-181 producing isolates. However, OXA-66 indicated greater binding stability and affinity for imipenem and meropenem. This study indicated structural/functional convergence as well as divergence among several carbapenemase variants and provided useful insights into carbapenemase-mediated carbapenem resistance that might help in identifying appropriate treatment regimen for bacterial infections.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Pal A,Bhattacharyya I,Tripathi Adoi
10.1007/s11274-020-02905-3subject
Has Abstractpub_date
2020-07-26 00:00:00pages
129issue
9eissn
0959-3993issn
1573-0972pii
10.1007/s11274-020-02905-3journal_volume
36pub_type
杂志文章abstract::Trichoderma harzianum produced RNA-depolymerase with maximum activity after 72 to 120 h of growth. Addition of K2HPO4 repressed enzyme production by the fungus. The optimal activity was at pH 7.8 and 40 to 50°C. The enzyme was stable at pH 3.2 to 9.0 and 80% of activity remained after 60 min at 40°C. EDTA and p-chloro...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00383067
更新日期:1993-05-01 00:00:00
abstract::Aquilaria malaccensis produces agarwood in response to wounding and fungal attack. However, information is limited regarding Aquilaria's interaction with its diverse fungal community. In this study, time-related changes of three natural fungal colonizers in two wounded wild A. malaccensis were tracked, beginning a few...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1668-2
更新日期:2014-09-01 00:00:00
abstract::Cellulolytic fungi, 34 strains, were isolated from samples taken from palm oil mill residues and effluent, and high cellulase producers selected in comparison with nine known reference strains. Although 13 isolates showed good filter paper distintegration within 14 days, only eight isolates exhibited clearing zones ar...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01238799
更新日期:1992-11-01 00:00:00
abstract::A strain of Aspergillus niger isolated from sugarcane fields, produced an extracellular transfructosylase in the culture medium. Sucrose and raffinose induced the production to the enzyme, which was purified by 138-fold. The optimum pH for activity and stability were 5.5 and 6.5, respectively. Its optimum temperature ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00329399
更新日期:1991-05-01 00:00:00
abstract::Bacillus coagulans, when grown on casein at 20°C, produced an inducible, metalloprotease of 28 kDa at 1.6 U/mg cell protein. (NH4)2SO4 at 2 g/l decreased enzyme production irrespective of carbon source. ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00414882
更新日期:1994-05-01 00:00:00
abstract::This study was conducted to report the richness of endophytic Penicillium and Talaromyces species isolated from Tillandsia catimbauensis, a bromeliad endemic in the Brazilian tropical dry forest (Caatinga), to verify their ability to produce the enzyme L-asparaginase and to partially optimise the production of biomass...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-018-2547-z
更新日期:2018-10-28 00:00:00
abstract::Ergosterol is the predominant nature sterol constituent of plasma membrane in Saccharomyces cerevisiae. Herein, the biosynthetic pathway of ergosterol was proposed to be metabolically engineered for the efficient production of ergosta-5,7-dien-3β-ol, which is the precursor of vitamin D4. By target disruption of erg5, ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-018-2440-9
更新日期:2018-03-28 00:00:00
abstract::Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, is an obligate intra-cellular bacterium that survives in neutrophils by delaying apoptosis. The human promyelocytic leukemia cell line HL-60 has been the ultimate choice for culturing Anaplasma in vitro. In this study, we assessed the v...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0745-z
更新日期:2011-11-01 00:00:00
abstract::As an interspecies and interkingdom signaling molecule, indole has recently received attention for its diverse effects on the physiology of both bacteria and hosts. In this study, indole increased the tetracycline resistance of Vibrio splendidus. The minimal inhibitory concentration of tetracycline was 10 μg/mL, and t...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-020-02813-6
更新日期:2020-02-22 00:00:00
abstract::Rhodotorula glutinis degraded variously(14)C-labelled synthetic lignins in the presence of 0.1% glucose as co-substrate. Side chain-labelled DHP was degraded the most. While this yeastutilized vanillate and forulate for growth, sinapate/syringate were poorly or not degraded. Gallate, protocatechuate and acetate also s...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01225355
更新日期:1990-03-01 00:00:00
abstract::Over a 3-week period, samples of fresh, chopped, pork meat were taken every morning and afternoon from 50 meat stalls. Microbiological examination revealed that the samples had (c.f.u./g): total microbes, 1×10(3) to 2.14×10(6); mean probable numbers of coliforms and Escherichia coli, 1.51×10(3) to 1.15×10(4); and yeas...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00361025
更新日期:1995-11-01 00:00:00
abstract::Eighteen non-sibling mutants of Pseudomonas aeruginosa PAO were isolated that were deficient in the utilization of the β-methyl branched acid citronellic acid but not in the utilization of the unbranched n-octanoic acid (Cau mutants). These mutants are also deficient in the utilization of citronellol and citronellal. ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00386294
更新日期:1993-09-01 00:00:00
abstract::Prebiotic Fructooligosaccharides (FOS) escape metabolism in upper GI tract undergo microbial metabolism in colon and thereby influence the nature, type and number of intestinal microbiota to improve host's health. The present study focuses on the ability of Lactobacillus plantarum CFR 2194 to utilize FOS as a selectiv...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0887-z
更新日期:2012-03-01 00:00:00
abstract::The methylotrophic yeast Pichia pastoris is widely used in recombinant expression of eukaryotic proteins owing to the ability of post-translational modification, tightly regulated promoters, and high cell density fermentation. However, episomal plasmids for heterologous gene expression and the CRISPR/Cas9 system for g...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2654-5
更新日期:2019-05-27 00:00:00
abstract::The fungal genus Ustilago consists of intimidating pathogens associated with disease manifestations in plants of agricultural importance and gravity. Rapid progress of genome sequencing has opened the floodgates for biological research. Availability of Ustilago genomes provides a scope to explore complex codon and ami...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2693-y
更新日期:2019-07-22 00:00:00
abstract::Soaking seeds of cucumber and pumpkin with an extract of Westiellopsis prolifica, an N2-fixing cyanobacterium, promoted germination and their subsequent growth and development. An extract of Lyngbya sp., a non-N2-fixing cyanobacterium, had no significant effect. ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00452845
更新日期:1991-11-01 00:00:00
abstract::When various lignin-related para-phenolic benzoic acids, para-phenolic cinnamic acids, para-phenolic phenylpropionic acids, the corresponding unsubstituted and 4-O-methylated derivatives, and 4-hydroxyl substituted benzaldehydes were tested on the growth of eight white-rot fungi, methylation of the 4-hydroxy substitue...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00360880
更新日期:1994-03-01 00:00:00
abstract::The mushroom Flammulina velutipes and the white-rot fungus Trametes versicolor were cultivated separately on sugarcane bagasse for 40 days. Trametes versicolor produced laccase and manganese-peroxidase activities, showing a simultaneous degradation of lignin and holocellulose. However, only phenoloxidase activity was ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00286370
更新日期:1995-09-01 00:00:00
abstract::The establishment of safe and effective methods for controlling fungal disease is an urgent issue in agriculture and forestry. Microbiological control of plant disease is expected to achieve better results than use of chemically derived fungicides. This study aimed to establish Brevibacillus laterosporus JX-5 as a pot...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-015-1912-4
更新日期:2015-10-01 00:00:00
abstract::The volatile compounds produced by Debaryomyces hansenii NRRL Y-7426 during the fermentation of detoxified concentrated distilled grape marc hemicellulosic hydrolysates was analysed by GC-MS. Thirty-five compounds corresponding to ten groups of volatile compounds: terpenes, higher alcohols, C₆ alcohols, aldehydes, vol...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1122-2
更新日期:2012-11-01 00:00:00
abstract::Shewanella colwelliana, a marine bacterium isolated in association with the oyster Crassostrea virginica, produces an abundant exopolysaccharide with potential commercial value as an adhesive under aqueous conditions. Its utilization of glucose was modulated by stoichiometric concentrations of yeast extract. In Brain ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00367663
更新日期:1994-09-01 00:00:00
abstract::Diarrhoea due toVibrio cholerae non-01 is common in Bangladesh. Four hundred and eighty samples, including plants, water, phytoplankton and sediment, were collected from five ponds in Dhaka every 15 days for one year.V. cholerae non-01 was isolated from 181 (38%) of the samples. Two peaks were evident: one in April an...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01195838
更新日期:1992-03-01 00:00:00
abstract::Amylase and pectin methyl esterase activities increased rapidly during the early period of the fermentation of cassava for 'fufu' production, attaining their peak activities after 12 and 24h, respectively. Cellulase activity was lower and approximately constant for most of the fermentation period. ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01200690
更新日期:1992-01-01 00:00:00
abstract::Two Clostridium thermocellum strains were improved for ethanol tolerance, to 5% (v/v), by gradual adaptation and mutation. The best mutant gave an ethanol yield of 0.37 g/g substrate, with a growth yield 1.5 times more than its parent. Accumulation of acids and reducing sugars by the mutant strain with 5% (v/v) ethano...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00327802
更新日期:1996-01-01 00:00:00
abstract::Antimicrobial activity of silver nanoparticles (AgNPs) has been well documented in earlier studies. As their efficient role in combating phytopathogens has begun recently, there is a huge scope to explore their effectiveness in agriculture. Considering the strong antifungal activity of biosynthesized AgNPs (as reporte...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-020-02826-1
更新日期:2020-03-16 00:00:00
abstract::Many important food and fibre crops have attained close to their maximum yields as a result of conventional breeding approaches and advances in agronomic and horticultural practices. The manipulation of cell and tissue cultures to produce somatic embryos efficiently is one of the keystones of the new technologies that...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00364617
更新日期:1995-07-01 00:00:00
abstract::When cell membranes of Lentinus edodes mycelium were rapidly frozen at either 50 or 160°C/min, viability was lost and this correlated with rupture of the plasmalemma and residual membrane material and with alterations in the organelles. Although with slow cooling (1°C/min) 80% of the samples recovered viability, some ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00369571
更新日期:1993-11-01 00:00:00
abstract::The economics of bioflocculant production is coupled with the use of a low-cost substrate at appropriate culture conditions. The use of a waste substrate for this purpose offers an additional treatment measure to mitigate environmental pollution. We investigated the growth of Aspergillus flavus and its bioflocculant y...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2696-8
更新日期:2019-07-22 00:00:00
abstract::Screening for spore-forming bacteria to use against dipterous insects, namelyMusca domestica, Aedes aegypti andCulex pipiens from Egypt, led to two isolates, M1 and S128, being found which were highly toxic to bothAedes andCulex strains. They had calculated international Toxicity Units (ITU) valdes of 17,467 and 20,06...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01201301
更新日期:1990-09-01 00:00:00
abstract::Fossil fuels consumption impacts the greenhouse gas emissions. Biofuels are considered as alternative renewable energy sources to reduce the fossil fuels dependency. Bioethanol produced by recombinant microorganisms is a widely suggested alternative to increase the yield in fermentation processes. However, ethanol and...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2769-8
更新日期:2019-11-20 00:00:00