Abstract:
:A method is described for the assessment of the fraction of microcytes from the red blood cell (RBC) size distribution histogram. For quantification of the fraction of microcytes an upper threshold corresponding with a RBC volume of 70 fL is established. The reference interval for the fraction of microcytes covers a range from 0.01 to 0.09. In order to examine the relationship between the fraction of microcytes and the mean cell volume (MCV) values computer simulation studies were performed. The computer simulations are based on a Gaussian distributed reference RBC histogram as generated on a Technicon H 6000/H 601 Hematology Analyser. From our studies it is shown that determination of the fraction of microcytes yields a higher sensitivity than MCV values for detecting small populations of microcytes. In particular, measurement of the fraction of microcytes is very sensitive to minor changes in the MCV values if a normocytic RBC population combined with a microcytic RBC fraction shows a wide dispersion in their cell volumes.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Bartels PC,Helleman PW,Soons JBdoi
10.1177/000456328802500615subject
Has Abstractpub_date
1988-11-01 00:00:00pages
680-7eissn
0004-5632issn
1758-1001journal_volume
25 ( Pt 6)pub_type
杂志文章abstract::A microtiter plate based Dual Analyte enzyme-immunoassay method for the simultaneous measurement of alpha-fetoprotein (AFP) and Free-beta human chorionic gonadotrophin (hCG) was evaluated. This rapid assay, which has application in both Neural Tube Defect screening and Down's screening, shows good precision with betwe...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900403
更新日期:1992-07-01 00:00:00
abstract::It is crucially important to detect subarachnoid haemorrhage (SAH) in all patients in whom it has occurred to select patients for angiography and preventative surgery. A computerized tomography (CT) scan is positive in up to 98% of patients with SAH presenting within 12 h, but is positive in only 50% of those presenti...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.007257
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVE:The menstrual cycle-related changes in clinical laboratory values were analysed by use of data obtained in the Asian multicentre study aimed at derivation of common reference intervals for 85 major clinical laboratory tests. METHODS:Among 1876 healthy female volunteers, 893 had regular menstruation. They wer...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215617212
更新日期:2016-05-01 00:00:00
abstract::Background Red blood cell distribution width (RDW) and neutrophil to lymphocyte ratio (NLR) have been reported to be associated with outcomes of acute cerebral infarction. However, their prognostic value in patients with subarachnoid haemorrhage (SAH) remains largely unknown. The aim of this study was to investigate t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563216686623
更新日期:2017-11-01 00:00:00
abstract::Using a high-performance liquid chromatography method, we measured seven commonly prescribed benzodiazepines (chlordiazepoxide, nitrazepam, nordiazepam, oxazepam, lorazepam, temazepam and diazepam) in 100 urine samples obtained from patients attending the Leeds Addiction Unit. All of the urines selected for investigat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329703400110
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:It is recommended that children receiving intravenous fluids should have frequent biochemical monitoring, in some situations 4-6 hourly. Small changes in sodium must be detected, requiring very high precision from sodium analyses. Some children are monitored using venous blood analysed by indirect ion-select...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456306778904560
更新日期:2006-11-01 00:00:00
abstract::The standard colorimetric methods most often used for the measurement of serum unsaturated iron binding capacity (UIBC) are subject to gross interference by iron dextran. This paper describes a brief evaluation of an alternative radiometric assay for serum UIBC, based on a commercially available kit method, but incorp...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500323
更新日期:1988-05-01 00:00:00
abstract:BACKGROUND:For nearly 20 years, we and others have used a three-parameter power function as a direct estimation error model for immunoassays. The main application is imprecision profile plots (after translating from variance to coefficient of variation) but other uses include weighting functions for regression analysis...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.007230
更新日期:2008-09-01 00:00:00
abstract::Parenteral nutrition is a well-established technique for feeding patients with intestinal failure and other conditions which render enteral feeding impossible. Biochemical tests are required in the initial assessment of these patients, for monitoring their response to treatment and for the detection and management of ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456328702400401
更新日期:1987-07-01 00:00:00
abstract::Hereditary renal hypouricaemia (HRH) is a genetic disorder commonly associated with exercise-induced acute kidney injury (AKI). We report the case of a 19-year-old man who was admitted to hospital with exercise-induced AKI and who was subsequently shown to have HRH. We believe this to be the first description of a cas...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2012.012017
更新日期:2013-05-01 00:00:00
abstract::The effect of progestins on intracellular corticosteroid-binding globulin (CBG) mRNA expression in an endometrial cancer cell line (Ishikawa) was examined in an attempt to understand the biological effects of high-dose progestins in the treatment of well-differentiated uterine endometrial cancers. Oestradiol-17 beta (...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500507
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Apolipoprotein concentrations in reflex tears from healthy and noninsulin-dependent diabetes mellitus (NIDDM) subjects were measured and correlated with the stage of diabetic retinopathy. METHODS AND RESULTS:The apolipoprotein A-I (apo A-I) concentrations in the tears from NIDDM patients with retinopathy we...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563021901748
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:We evaluated procalcitonin (PCT) assay in the emergency diagnosis of neonatal bacterial infection, especially in preterm infants, relative to C-reactive protein (CRP) and fibrinogen. METHODS:One hundred and twenty neonates (32 preterm), of whom 21 were infected, were tested. RESULTS:Concentrations of PCT, ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563021901874
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Phlebotomy is a potential cause of preanalytical errors. We have observed phlebotomy in routine practice in a busy Emergency Department, to see how current practice compares with optimal blood sampling. METHODS:Phlebotomy episodes were audited and compared with standard procedures. A computer-based search o...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2011.011099
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Glycated haemoglobin (HbA1c) concentration is lower relative to glyacemic control in diabetic patients on haemodialysis. However, it is unknown as to whether this is also true for diabetic patients with end-stage renal disease but not on haemodialysis. METHODS:Correlations between HbA1c or glycated albumin ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2011.011161
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Since more than 90% of cortisol is bound to protein, serum free cortisol (SFC) may be a more appropriate marker of adrenal status than total cortisol. However, measurement of SFC is technically difficult and calculated SFC may offer a more practical alternative. METHODS:SFC, measured by equilibrium dialysis...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2010.009246
更新日期:2010-05-01 00:00:00
abstract::Measurement of urinary free cortisol using the Bayer Automated Chemiluminescent System (ACS:180 PLUS) was evaluated and compared with an in-house extraction radioimmunoassay (RIA). Inter-assay coefficients of variation were acceptable, being respectively 5.3%, 4.8% and 3.8% at 141, 406 and 942 nmol/L (n=20) for the AC...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456320003700414
更新日期:2000-07-01 00:00:00
abstract::Plasma immunoreactive alpha-atrial natriuretic peptide (IR alpha-ANP) levels were determined before, and at 5 and 10 min after bolus intravenous administration of morphine (0.15 or 0.30 mg/kg) in 21 otherwise healthy human subjects who underwent elective surgery. Five min after injection IR alpha-ANP levels had nearly...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700105
更新日期:1990-01-01 00:00:00
abstract::A comparison of the Roche Cobas Core and Hybritech TANDEM-E PSA (prostate specific antigen) assays revealed a large difference in the reactivity of each assay to the separated free and complexed forms of serum PSA in patients with prostatic carcinoma. The Roche assay was relatively much more responsive to the free for...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329403100514
更新日期:1994-09-01 00:00:00
abstract:BACKGROUND:Various conjugated cholesterol metabolites are excreted in urine of the patients with metabolic abnormalities and hepatobiliary diseases. We aimed to examine the usefulness of precursor ion scan and neutral loss scan for the characterization of conjugated cholesterol metabolites in urine. METHODS:A mixture ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214568871
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:Potassium is usually the most important analyte affected by in vitro haemolysis and the result obtained may falsely indicate or disguise a life-threatening abnormality and so give rise to inappropriate treatment. The purpose of the study was to provide a solution to the problem of reporting potassium on haem...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563053492739
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:Interpretative commenting constitutes an important aspect of the post-analytical phase in chemical pathology, but has only recently been the subject of quality assessment. The Royal College of Pathologists of Australasia (RCPA)-Australasian Association of Clinical Biochemists (AACB) Chemical Pathology Patien...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563021901955
更新日期:2002-05-01 00:00:00
abstract::The primary antibody deficiency syndromes are a group of rare disorders characterized by an inability to produce clinically effective immunoglobulin responses. Some of these disorders result from genetic mutations in genes involved in B cell development, whereas others appear to be complex polygenic disorders. They mo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/acb.2008.008175
更新日期:2009-03-01 00:00:00
abstract::The levels of thyroid hormones in paired venous and capillary serum samples taken from 68 healthy full-term neonates were determined by radioimmunoassay. Compared with capillary values, total thyroxine (T4), thyrotropin (TSH) and thyroxine-binding globulin (TBG) each showed a significant decrease in venous serum. The ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328402100413
更新日期:1984-07-01 00:00:00
abstract::Insulin resistance is a common condition, recognized to be a central feature of the metabolic syndrome, and strongly associated with an increased risk of cardiovascular disease and diabetes. The quantitative assessment of insulin sensitivity is not used for routine clinical purposes, but the emerging importance of ins...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/000456307780945778
更新日期:2007-07-01 00:00:00
abstract::The Poser criteria for diagnosing multiple sclerosis (MS) includes clinical, paraclinical and laboratory information. We studied the influence of cerebrospinal fluid (CSF) biochemistry results on the categorisation of patients with suspected MS. A retrospective study was made of 138 patients who had CSF samples sent o...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700303
更新日期:1990-05-01 00:00:00
abstract::The effects of inhibition of inosine monophosphate dehydrogenase (IMPDH), the rate-limiting enzyme in guanine nucleotide de novo synthesis, on cell growth, cell viability, endogenous nucleotide concentrations and concentrations of extracellular nucleosides and bases were studied in Molt F4 human malignant lymphoblasts...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329403100211
更新日期:1994-03-01 00:00:00
abstract::During incubation with rabbit blood in vitro rabbit-muscle lactate dehydrogenase-5 was inactivated at a rate similar to that observed in vivo. By contrast plasma and plasma containing erythrocytes had no effect on the enzyme activity, but plasma containing leucocytes inactivated the enzyme at the same rate as whole bl...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327701400109
更新日期:1977-01-01 00:00:00
abstract::Pancreatic elastase 1 (E1), a digestive protease, is synthesized by the acinar cells of the pancreas. Using an enzyme-linked immunosorbent assay, we evaluated stool E1 levels in the following groups of patients. (a) Specimens submitted for occult blood examination from 20 adults, over 3 consecutive days, to assess the...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329903600606
更新日期:1999-11-01 00:00:00
abstract::The seasonal variation of serum 25-hydroxy vitamin D3 and 1,25-dihydroxy-vitamin D has been investigated. Blood was taken from 27 healthy volunteers, aged 21-44 years old at 3 monthly intervals over a period of 1 year. A scrolling monthly programme with 12 quarterly (3 month) time periods was developed. A summer assoc...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329403100105
更新日期:1994-01-01 00:00:00