Abstract:
:Melatonin is produced following circadian rhythm with high levels being released at night and has been implicated in the regulation of physiological processes in major tissues, including the pancreas. The aim of our study was to examine the effects of melatonin on intracellular free Ca(2+) concentration ([Ca(2+) ](c)) in AR42J pancreatic cells. Our results show that stimulation of cells with 1 nm cholecystokinin (CCK)-8 led to a transient increase in [Ca(2+) ](c) followed by a decrease towards a value close to the prestimulation level. Melatonin (at the concentrations 1, 10, 100 μm and 1 mm) induced changes in [Ca(2+) ](c) that consisted of single or short lasting spikes in the form of oscillations or slow transient increases followed by a slow reduction towards a value close to the resting level. Depletion of intracellular Ca(2+) stores by stimulation of cells with 1 nm CCK-8 or 1 μm thapsigargin (Tps) blocked Ca(2+) responses evoked by melatonin in the majority of cells. Conversely, prior stimulation of cells with 1 mm melatonin in the absence of extracellular Ca(2+) inhibited Ca(2+) mobilization in response to a secondary application of CCK-8 or Tps. In summary, our results show that melatonin releases Ca(2+) from intracellular stores and can therefore modulate the responses of the pancreas to CCK-8. The source for Ca(2+) mobilization most probably is the endoplasmic reticulum. These data raise the possibility that melatonin also involves Ca(2+) signalling, in addition to other intracellular messengers, to modulate cellular function.
journal_name
J Pineal Resjournal_title
Journal of pineal researchauthors
del Castillo-Vaquero A,Salido GM,Gonzalez Adoi
10.1111/j.1600-079X.2010.00790.xsubject
Has Abstractpub_date
2010-10-01 00:00:00pages
256-63issue
3eissn
0742-3098issn
1600-079Xpii
JPI790journal_volume
49pub_type
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
pub_type: 杂志文章
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abstract::Our objective was to suppress the daily surge of melatonin in serum of prepubertal dairy heifers by manipulating intensity of light (Experiment 1) and duration of exposure to light (Experiment 2). Heifers in Experiment 1 were exposed to either 12 hr of darkness (000 lux, control), or 400, 800, or 1,200 lux of light du...
journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
pub_type: 杂志文章,评审
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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更新日期:2009-09-01 00:00:00
abstract::Recent studies implicate melatonin in the antinociceptive activity of sensory neurons. However, the underlying mechanisms are still largely unknown. Here, we identify a critical role of melatonin in functionally regulating Cav3.2 T-type Ca2+ channels (T-type channel) in trigeminal ganglion (TG) neurons. Melatonin inhi...
journal_title:Journal of pineal research
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更新日期:2018-05-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1991.tb00472.x
更新日期:1991-10-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1034/j.1600-079x.2003.00083.x
更新日期:2003-11-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1988.tb00782.x
更新日期:1988-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章,评审
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更新日期:2020-04-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
abstract::The quantitative autoradiographic 2-[14C]deoxyglucose method was employed to map the metabolic activity of the superior cervical ganglion and the entire brain during unilateral electrical stimulation of the cervical sympathetic trunk in the urethane-anesthetized rat. Stimulation of the cervical sympathetic trunk incre...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1988.tb00768.x
更新日期:1988-01-01 00:00:00
abstract::Melatonin, the main secretary product of the pineal gland, is potentially effective in the prevention of a number of diseases in which free radical processes are involved. The development of hypercholesterolemia is a multifactorial process in which elevated oxidized low-density lipoprotein (ox-LDL) levels play a centr...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2008.00595.x
更新日期:2008-10-01 00:00:00