Abstract:
:Polyacrylamide gel electrophoresis under native conditions (native PAGE) is a well-established and versatile method for probing nucleic acid conformation and nucleic acid-protein interactions. Native PAGE has been used to measure RNA folding equilibria and kinetics under a wide variety of conditions. Advantages of this method are its adaptability, absolute determination of reaction endpoints, and direct analysis of conformational hetereogeneity within a sample. Native PAGE is also useful for resolving ligand-induced structural changes.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Woodson SA,Koculi Edoi
10.1016/S0076-6879(09)69009-1subject
Has Abstractpub_date
2009-01-01 00:00:00pages
189-208eissn
0076-6879issn
1557-7988pii
S0076-6879(09)69009-1journal_volume
469pub_type
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