Abstract:
:The Arc (anoxic redox control) two-component system (TCS) is a complex signal transduction system that plays an important role in regulating energy metabolism at the level of transcription in bacteria. This system comprises the ArcB protein, a hybrid membrane-associated sensor kinase, and the ArcA protein, a typical response regulator. Under anoxic growth conditions, ArcB autophosphorylates and transphosphorylates ArcA via a His→Asp→His→Asp phosphorelay. Under aerobic conditions, the ArcB kinase activity is silenced by the oxidation of two cytosol-located redox-active cysteine residues that participate in intermolecular disulfide bond formation. Under such conditions, ArcB acts as a phosphatase that catalyzes the dephosphorylation of ArcA-P and thereby releasing its transcriptional regulation. This chapter describes general in vitro and in vivo assays and strategies that have been used to characterize the ArcB/A two-component signal transduction system, which could, also, be applied to most other TCS.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Alvarez AF,Georgellis Ddoi
10.1016/S0076-6879(10)71012-0subject
Has Abstractpub_date
2010-01-01 00:00:00pages
205-28eissn
0076-6879issn
1557-7988pii
S0076-6879(10)71012-0journal_volume
471pub_type
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