Genetic Manipulation of Neurofilament Protein Phosphorylation.

Abstract:

:Neurofilament biology is important to understanding structural properties of axons, such as establishment of axonal diameter by radial growth. In order to study the function of neurofilaments, a series of genetically modified mice have been generated. Here, we describe a brief history of genetic modifications used to study neurofilaments, as well as an overview of the steps required to generate a gene-targeted mouse. In addition, we describe steps utilized to analyze neurofilament phosphorylation status using immunoblotting. Taken together, these provide comprehensive analysis of neurofilament function in vivo, which can be applied to many systems.

journal_name

Methods Enzymol

journal_title

Methods in enzymology

authors

Jones MR,Villalón E,Garcia ML

doi

10.1016/bs.mie.2015.07.027

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

461-76

eissn

0076-6879

issn

1557-7988

pii

S0076-6879(15)00431-0

journal_volume

568

pub_type

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