Abstract:
:Neurofilament biology is important to understanding structural properties of axons, such as establishment of axonal diameter by radial growth. In order to study the function of neurofilaments, a series of genetically modified mice have been generated. Here, we describe a brief history of genetic modifications used to study neurofilaments, as well as an overview of the steps required to generate a gene-targeted mouse. In addition, we describe steps utilized to analyze neurofilament phosphorylation status using immunoblotting. Taken together, these provide comprehensive analysis of neurofilament function in vivo, which can be applied to many systems.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Jones MR,Villalón E,Garcia MLdoi
10.1016/bs.mie.2015.07.027subject
Has Abstractpub_date
2016-01-01 00:00:00pages
461-76eissn
0076-6879issn
1557-7988pii
S0076-6879(15)00431-0journal_volume
568pub_type
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