Abstract：
:A combined bead milling and enzymatic hydrolysis process was developed for fractionation of the major valuable biomass components, i.e., proteins, carbohydrates, and lipids from the microalgae Chlorella vulgaris. The cells were treated by bead milling followed by hydrolysis with different hydrolytic enzymes, including lipase, phospholipase, protease, and cellulase. Without enzymatic hydrolysis, the recovery yield of lipids, carbohydrates, and proteins for bead milled biomass was 75%, 31%, and 40%, respectively, while by applying enzymatic treatments these results were improved significantly. The maximum recovery yield for all components was obtained after enzymatic hydrolysis of bead milled biomass by lipase at 37 °C and pH 7.4 for 24 h, yielding 88% lipids in the solid phase while 74% carbohydrate and 68% protein were separated in the liquid phase. The recovery yield of components after enzymatic hydrolysis of biomass without bead milling was 44% lower than that of the milled biomass.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Alavijeh RS,Karimi K,Wijffels RH,van den Berg C,Eppink Mdoi
10.1016/j.biortech.2020.123321subject
Has Abstractpub_date
2020-08-01 00:00:00pages
123321eissn
0960-8524issn
1873-2976pii
S0960-8524(20)30593-9journal_volume
309pub_type
杂志文章abstract：:Fed-batch enzymatic hydrolysis process from alkali-pretreated sugarcane bagasse was investigated to increase solids loading, produce high-concentration fermentable sugar and finally to reduce the cost of the production process. The optimal initial solids loading, feeding time and quantities were examined. The hydrolys...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.05.034
更新日期：2014-09-01 00:00:00
abstract：:Butyrate fermentation by immobilized Clostridium tyrobutyricum was successfully carried out in a fibrous bed bioreactor using cane molasses. Batch fermentations were conducted to investigate the influence of pH on the metabolism of the strain, and the results showed that the fermentation gave a highest butyrate produc...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.02.032
更新日期：2009-07-01 00:00:00
abstract：:Organosolv is a promising pretreatment for lignocellulose biorefinery on the integrated utilization of full components from lignocellulosic biomass. A highly efficient pretreatment process using methanol/dioxane binary solvent with microwave irradiation is proposed in this study. Poplar wood was fractionated to high q...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122657
更新日期：2020-03-01 00:00:00
abstract：:One way envisioned to overcome part of the issues biodiesel production encounters today is to develop a simple, economically viable and eco-friendly process for the extraction of lipids from microalgae. This study investigates the lipid extraction efficiency from the microalga Chlamydomonas reinhardtii as well as the ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.01.139
更新日期：2018-06-01 00:00:00
abstract：:Triiodide resin (TR) was used to generate axenic cultures of microalgae by employing the antibacterial capability of triiodide. A Nannochloropsis gaditana culture contaminated with bacteria was passed through a column filled with TR using the gravity flow. Based on analyses of flow cytometry and vital staining using a...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.03.082
更新日期：2015-09-01 00:00:00
abstract：:The aim of this work was to improve sugarcane bagasse thermochemical conversion to pyrolytic sugars production, particularly to levoglucosan. The experiments were carried out evaluating the effect of acid washing with HNO3 (0.1wt.%) followed by H2SO4 addition (0.1, 0.2 and 0.3wt.%) at pyrolysis temperatures of 350, 40...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.10.051
更新日期：2017-01-01 00:00:00
abstract：:The nematicidal potential of culture filtrates of the blue-green alga, Microcoleus vaginatus (Cyanobacterium) was tested against Meloidogyne incognita on tomato in pots under greenhouse conditions. Prior to the transplantation of tomato seedling, roots were dipped in different concentrations (0.2%, 0.5%, 1%, 2%, 10%, ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2004.11.012
更新日期：2005-08-01 00:00:00
abstract：:The improvement of photosynthetic efficiency in a 100 m2 raceway reactor by enhancement of light regime to which the cells are exposed is here reported. From Computational Fluid Dynamics it was calculated that the light exposure times ranged from 0.4 to 3.6 s while the exposure times to darkness were much longer, from...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.02.032
更新日期：2019-05-01 00:00:00
abstract：:Multiple bacterial strains with CBA metabolic properties were isolated using a simple selective strategy. Phylogenetic analysis of the 16S rRNA gene sequences grouped them into two main clusters consisting of four bacterial phyla and belonging to 17 genera. Whereas growth was more frequent with 2-CBA (∼68%), 50% grew ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.10.026
更新日期：2011-02-01 00:00:00
abstract：:Among various forest biowastes, chestnut bur had the highest uptake values of Cd(II) and Pb(II), and these values were higher than those of agricultural biowastes used as comparable biosorbents. This study is the first report showing the high potential of chestnut bur as biosorbent for the removal of cationic heavy me...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.10.092
更新日期：2015-01-01 00:00:00
abstract：:The influence of free air space (FAS) on passively aerated composting has been reported, but the quantitative relationship between FAS and the microbial kinetics in passively aerated compost has not been investigated. This relationship was studied by composting dairy manure and straw in an enclosed, passively aerated,...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.06.051
更新日期：2009-01-01 00:00:00
abstract：:The aim of this study was to obtain experimental data related to liquid–liquid equilibrium (LLE) of systems containing glycerol + ethanol + ethyl biodiesel from macauba pulp oil, perform thermodynamic modeling and simulate the settling step of this biodiesel using simulation software. Binary interaction parameters wer...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.12.190
更新日期：2013-03-01 00:00:00
abstract：:The dehydration of fructose or glucose to 5-hydroxymethylfurfural (5-HMF) using room temperature ionic liquids (ILs) as a solvent is a promising method for producing liquid fuels from renewable resources. The IL, 1-hydroxyethyl-3-methylimidazolium tetrafluoroborate ([C(2)OHMIM]BF(4)), was used a catalyst-rather than a...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.06.081
更新日期：2012-10-01 00:00:00
abstract：:Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.10.042
更新日期：2013-05-01 00:00:00
abstract：:This research investigated the calcium effect on the anaerobic treatment of fresh leachate in an expanded granular sludge bed (EGSB) bioreactor under mesophilic conditions. The observations show that the bioreactor, inoculated with anaerobic granular sludge, can be started up only in about 40 days for the treatment of...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.11.056
更新日期：2011-05-01 00:00:00
abstract：:The effect of initial glucose concentration, initial pH and nickel nanoparticles concentration on biohydrogen production was experimented at mesophilic temperature (30-35 °C) using anaerobic microflora in batch tests. It revealed that yield of biohydrogen using nickel nanoparticles with an average size of 13.64 nm was...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.03.082
更新日期：2013-08-01 00:00:00
abstract：:A packed cage rotating biological contactor (RBC) system was applied to treat wastewater containing Cl2 residue with concentration even up to 20 mg/L. However, Cl2 exhibited a negative effect on the efficiency of the system as evidenced by the decrease in the growth of bio-film. It could be concluded that the removal ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.07.030
更新日期：2006-09-01 00:00:00
abstract：:The feasibility of using synthetic kitchen waste (KW) and fat, oil, and grease (FOG) as co-substrates in the anaerobic digestion of waste activated sludge (WAS) was investigated using two series of biochemical methane potential (BMP) tests. Ranges of ideal substrate to inoculum (S/I) ratio were determined for the FOG ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.07.103
更新日期：2011-10-01 00:00:00
abstract：:In this study, pharmaceutical wastewater with high total dissolved solids (TDSs) and chemical oxygen demand (COD) content was treated through a sequential anaerobic-aerobic treatment process. For the anaerobic process, an up-flow anaerobic sludge blanket (UASB) was applied, and a COD removal efficiency of 41.3±2.2% wa...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.11.045
更新日期：2014-02-01 00:00:00
abstract：:Activated carbon (AC) prepared from waste Parthenium was used to eliminate Ni(lI) from aqueous solution by adsorption. Batch mode adsorption experiments are carried out, by varying contact time, metal ion concentration, carbon concentration, pH and desorption to assess kinetic and equilibrium parameters. They allowed ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/s0960-8524(01)00093-1
更新日期：2002-01-01 00:00:00
abstract：:Slurry samples, collected from 41 commercial swine farms in South Korea, were characterized in various physico-biochemical, macro and micronutrients, heavy metals and microbial parameters. Interestingly, significant variations were observed in all the parameters. However, positive relationships were noticed between EC...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.05.006
更新日期：2009-10-01 00:00:00
abstract：:The effects of Pb chelater (EDTA-Pb) and ionic Pb (Pb(NO(3))(2)) on root cell death, Pb accumulation, changes of ROS, activities of antioxidant enzymes and uptake of mineral elements in response to Pb toxicity in Sedum alfredii H. were compared. Loss of plasma membrane integrity became serious by increasing Pb concent...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.12.056
更新日期：2008-09-01 00:00:00
abstract：:The combustion of paper, fruit waste and plant residue mixtures representing the lignocellulosic materials of municipal solid waste (MSW) in different atmospheres (80N(2)/20O(2), 70N(2)/30O(2), 60N(2)/40O(2), 50N(2)/50O(2), 80CO(2)/20O(2), 70CO(2)/30O(2), 60CO(2)/40O(2), 50CO(2)/50O(2)) was analyzed thermogravimetrica...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.12.039
更新日期：2012-03-01 00:00:00
abstract：:The production of fatty acid methyl ester (FAME) by direct in situ alkaline-catalyzed transesterification of the triglycerides (TG) in cottonseeds was examined. The experimental results showed that the amount of cottonseed oil dissolved in methanol was approximately 99% of the total oil and the conversion of this oil ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.04.059
更新日期：2008-12-01 00:00:00
abstract：:In this study, an electrolysis-assisted down-flow constructed wetland (E-DFCW) was successfully established, and achieved simultaneously efficient removal of PO43--P (93.6% ± 3.2%), NO3--N (97.1% ± 2.0%) and TN (80.6% ± 5.4%). When compared with electrolysis-assisted up-flow constructed wetland (E-UFCW), E-DFCW allowe...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2021.124692
更新日期：2021-01-11 00:00:00
abstract：:Methanogenic bioreactors, which are packed with supporting material, have attracted attention as an efficient means of degrading garbage. We aimed to increase bioreactor performance by using an electrochemical system to regulate the electrical potential on supporting material. At an organic loading rate of 26.9g dichr...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.12.076
更新日期：2010-05-01 00:00:00
abstract：:In this study a complementary modeling and experimental approach was used to explore how growth controls the NADPH generation and availability, and the resulting impact on PHB (polyhydroxybutyrate) yields and kinetics. The results show that the anabolic demand allowed the NADPH production through the Entner-Doudoroff ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.08.120
更新日期：2013-11-01 00:00:00
abstract：:The validation of a control strategy for biogas upgrading via light-driven CO2 consumption by microalgae and H2S oxidation by oxidizing bacteria using the oxygen photosynthetically generated was performed in a semi-industrial scale (9.6 m3) photobioreactor. The control system was able to support CO2 concentrations low...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123207
更新日期：2020-07-01 00:00:00
abstract：:Sterilization, alkaline-thermal and acid-thermal treatments were applied to activated sludge and the pre-treated sludge was used as raw material for Rhodococcus R3 to produce polymeric substances. After 60 h of fermentation, bioflocculant of 2.7 and 4.2 g L(-1) were produced in sterilized and alkaline-thermal treated ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.06.003
更新日期：2013-09-01 00:00:00
abstract：:Quantitative recovery is necessary for scale-up application of acidic ionic liquids (AILs). Ultrafiltration and bipolar membrane electrodialysis (BMED) was employed for the recovery and regeneration of acidic ionic liquid 1-butyl-3-methylimidazolium hydrogen sulphate (Bmim[HSO4]) after biomass pretreatment. Ultrafiltr...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2021.124726
更新日期：2021-01-18 00:00:00
