Abstract：
:Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to completely consume 20 g/L of glucose to produce lactic acid at a yield of 95% in 1L MRS medium at pH 3.8 within 36 h, whereas no growth or lactic acid production was observed for the wild-type strain under the same conditions. The acid tolerance of mutant MT3 remained genetically stable for at least 25 subcultures. Therefore, the error-prone whole genome amplification technique is a very powerful tool for improving phenotypes of this lactic acid bacterium and may also be applicable for other microorganisms.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Ye L,Zhao H,Li Z,Wu JCdoi
10.1016/j.biortech.2012.10.042subject
Has Abstractpub_date
2013-05-01 00:00:00pages
459-63eissn
0960-8524issn
1873-2976pii
S0960-8524(12)01544-1journal_volume
135pub_type
杂志文章abstract：:The knowledge of the effect of aerobic starvation on the functional bacterial activities and community structures is important for the recovery of nutrient removal in activated sludge system. Four aerobic starvation processes (3, 7, 14 and 30days) for nitrifiers and polyphosphate accumulating organisms (PAOs) were stu...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.07.131
更新日期：2017-11-01 00:00:00
abstract：:Biogas production has been the focus of many individuals in the developing world; there have been several investigations that focus on improving the production process and product quality. In the developing world the lack of advanced technology and capital has hindered the development of energy production. Renewable e...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2017.12.013
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abstract：:Biocatalyst behavior was comparatively evaluated under diverse microenvironments viz., self-induced electrogenic (bioelectrochemical treatment, BET) and anaerobic treatment (AnT) microenvironments, with real-field pharmaceutical wastewater. Relatively higher treatment efficiency was observed with BET (COD removal, 78....
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.08.061
更新日期：2011-12-01 00:00:00
abstract：:Anaerobic digestion (AD) of lignocellulosic biomass has received significant attention for bioenergy production in recent years. However, hydrolysis is a rate-limiting in AD of such feedstock. In this study, effects of hydrothermal pretreatment of Napier grass, a model lignocellulosic biomass, on methane yield were ex...
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abstract：:Bioavailability of Nickel in the form of hydrated Nickel(II) attributes to its toxicological effects and hence its removal from aqueous solution is of great concern. Adsorption is used as an efficient technique for the removal of Nickel(II), hereafter Ni(II), from water and wastewaters. Activated carbon obtained from ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.08.069
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abstract：:The effects of acid protease and urea addition during the fermentation step were evaluated. The fermentations were also tested with and without the addition of urea to determine if protease altered the nitrogen requirements of the yeast. Results show that the addition of the protease had a statistically significant ef...
journal_title：Bioresource technology
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abstract：:Sulfamethoxazole (SMX) pollution in wastewater threatens public health. A novel membrane bioelectrochemical reactor (MBER) with loop operation was developed for SMX degradation in low-C/N ratio wastewater. A gas-permeable silicone membrane module was used to precisely control the dissolved oxygen in the catholyte and ...
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.08.038
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.05.016
更新日期：2006-07-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.11.063
更新日期：2009-11-01 00:00:00
abstract：:This study demonstrated the microbial purification of a model wastewater containing 2,4,6-trinitrophenol (TNP), which was carried out in a continuously working biological aerated filter (BAF). The main emphasis was on the operating performance of the reactor as a function of the pollution load. TNP was degraded at a m...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.10.018
更新日期：2009-03-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.05.048
更新日期：2008-05-01 00:00:00
abstract：:Recently, digestate disintegration gained interest as an alternative strategy to feedstock pretreatment for anaerobic digestion. This study evaluated the effect of three different digestate disintegration methods (hydrogen peroxidation, ozone treatment and ultrasound) on manure digestate, potato waste digestate and mi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.08.036
更新日期：2018-11-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122591
更新日期：2020-03-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.02.032
更新日期：2009-07-01 00:00:00
abstract：:The adsorption of divalent metal ions Cu2+, Pb2+, Zn2+, and Cd2+ on coffee grounds as a function of coffee grounds concentration was studied in which adsorption density decreased as the concentration of coffee grounds (C(s)) increased. Adsorption studies were conducted by equilibrating aqueous solutions of each metal ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.06.094
更新日期：2010-03-01 00:00:00
abstract：:The present work aims to address the gas-phase biotransformation of geraniol into citronellol using growing cells of Saccharomyces cerevisiae (baker's yeast) in a continuous-closed-gas-loop bioreactor (CCGLB). This study revealed that the gaseous geraniol had a severe effect on the production of biomass during the gro...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.07.053
更新日期：2011-10-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.biortech.2016.11.076
更新日期：2017-01-01 00:00:00
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更新日期：2017-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-12-01 00:00:00
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更新日期：2017-12-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.03.158
更新日期：2013-06-01 00:00:00
abstract：:This study demonstrates that increased NADPH production can improve biomass and ethanol production in cyanobacteria. We over-expressed the endogenous zwf gene, which encodes glucose-6-phosphate dehydrogenase of pentose phosphate pathway, in the model cyanobacterium Synechocystis sp. PCC 6803. zwf over-expression resul...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.02.056
更新日期：2016-08-01 00:00:00
abstract：:To overcoming the natural recalcitrance of cellulose for glucose production via enzymatic hydrolysis, a new strategy of destroying hydrogen bond donor to reconstruct cellulose's hydrogen bonding network was developed via a mild reversible reaction of cellulose with CO2 catalyzed by organic bases. The reaction dynamics...
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doi：10.1016/j.biortech.2019.122230
更新日期：2020-01-01 00:00:00
abstract：:Novel horizontal tubes and triangular prism (HTTP) baffles that generate flow vortices were developed to increase solution velocity between dark and light areas and thus improve microalgal growth in a flat-panel photo-bioreactor. Solution velocity, mass-transfer coefficient, and mixing time were measured with a partic...
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更新日期：2016-07-01 00:00:00
abstract：:Acetic acid was used in abiotic experiments to adjust the solution pH and investigate its influence on the chemical hydrolysis of the Organic Fraction of Municipal Solid Waste (OFMSW). Soluble chemical oxygen demand (SCOD) was used to measure the hydrolysis under oxidative conditions (positive oxidation-reduction pote...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.03.016
更新日期：2015-06-01 00:00:00
abstract：:A new biomimetic absorbent, cellulose acetate (CA) embedded with triolein (CA-triolein), was prepared and applied for the removal of persistent organic pollutants (POPs) from micro-polluted aqueous solution. The comparison of CA-triolein, CA and granular activated carbon (GAC) for dieldrin removal was investigated. Re...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.01.026
更新日期：2009-06-01 00:00:00
