Enhanced malic acid production using Aspergillus niger coupled with in situ product recovery.

Abstract:

:In the present investigation, recovery of malic acid from the fermentation broth was performed by using in situ reactive extraction method employing different combination of amine and solvent systems. Totally six solvent mixtures were tested for toxicity on Aspergillus niger PJR1. Further, effect of solvent mixture concentration on separation of malic acid was investigated. Solvent system consisting of 2 M of trioctylamine in 1-octanol was found to be non-toxic to A. niger PJR1 and resulted in the maximum partition coefficient of 0.75 when the solvent mixture to liquid ratio of 1:2 used. A. niger PJR1 from crude glycerol using in situ reactive batch fermentation resulted in the maximum malic acid titer of 115.67 ± 3.5 g/L with the productivity of 0.53 g/L.h after 216 h. Further, fed batch extractive fermentation with crude glycerol resulted in malic acid titer of 131.48 ± 3.4 g/L with the productivity of 0.45 g/L.h after 288 h. Thus reactive extraction combined with in situ fermentation could become effective method for enhanced malic acid production.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Iyyappan J,Baskar G,Bharathiraja B,Gopinath M

doi

10.1016/j.biortech.2020.123259

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

123259

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(20)30530-7

journal_volume

308

pub_type

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