Abstract:
:A new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated serologic assays are needed for contact tracing, identifying the viral reservoir, and epidemiologic studies. We developed serologic assays for detection of SARS-CoV-2 neutralizing, spike protein-specific, and nucleocapsid-specific antibodies. Using serum samples from patients with PCR-confirmed SARS-CoV-2 infections, other coronaviruses, or other respiratory pathogenic infections, we validated and tested various antigens in different in-house and commercial ELISAs. We demonstrated that most PCR-confirmed SARS-CoV-2-infected persons seroconverted by 2 weeks after disease onset. We found that commercial S1 IgG or IgA ELISAs were of lower specificity, and sensitivity varied between the 2 assays; the IgA ELISA showed higher sensitivity. Overall, the validated assays described can be instrumental for detection of SARS-CoV-2-specific antibodies for diagnostic, seroepidemiologic, and vaccine evaluation studies.
journal_name
Emerg Infect Disjournal_title
Emerging infectious diseasesauthors
Okba NMA,Müller MA,Li W,Wang C,GeurtsvanKessel CH,Corman VM,Lamers MM,Sikkema RS,de Bruin E,Chandler FD,Yazdanpanah Y,Le Hingrat Q,Descamps D,Houhou-Fidouh N,Reusken CBEM,Bosch BJ,Drosten C,Koopmans MPG,Haagmans BLdoi
10.3201/eid2607.200841subject
Has Abstractpub_date
2020-07-01 00:00:00pages
1478-1488issue
7eissn
1080-6040issn
1080-6059journal_volume
26pub_type
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