Effects of cigarette smoke condensate and nicotine on human gingival fibroblast-mediated collagen degradation.


BACKGROUND:Members of the matrix metalloproteinase (MMP) family have been shown to be involved in periodontal disease. Risk factors for periodontal disease include tobacco smoking. Cigarette smoke condensate (CSC) is comprised of thousands of chemicals. Nicotine is one of the active components in tobacco. This study compares the effects of CSC and nicotine at the level in CSC on the collagen-degrading ability of human gingival fibroblasts (HGFs) and the expression of selected MMPs and tissue inhibitors of metalloproteinases (TIMPs). METHODS:HGFs were seeded in six-well collagen-coated plates, exposed to 100 μg/mL (2.4 μg/mL nicotine) of CSC or 2.4 μg/mL nicotine for 3 days, and then collagen degradation was analyzed. After 3 days exposure to CSC or nicotine, the conditioned media from HGFs was collected and the membrane proteins were extracted for gelatin zymography and Western blot analyses. The mRNA levels of MMP-2, MMP-14, and TIMP-2 were measured by reverse transcription-polymerase chain reaction. RESULTS:The CSC increased collagen degradation, and increased the levels of TIMP-2, MMP-14, and the active MMP-2 in the membrane extracts, and their mRNA levels. CSC also increased the level of active MMP-2 in the conditioned media. Nicotine at the level in CSC (2.4 μg/mL) had little influence on collagen degradation, as well as on the protein and mRNA levels of MMP-2, MMP-14, and TIMP-2. CONCLUSIONS:CSC may increase HGF-mediated collagen degradation by affecting membrane-associated MMPs and TIMPs.


J Periodontol


Zhang W,Fang M,Song F,Windsor LJ




Has Abstract


2011-07-01 00:00:00












  • Effect of periodontal therapy on patients maintained for 15 years or longer. A retrospective study.

    abstract::A retrospective study of tooth loss in 211 patients who were treated for periodontal disease in private practice and maintained for 15 to 34 years on 3- to 6-month recall schedules is reported. The average age of the patients was 42 years, and the average length of time in maintenance was 22 years. On the basis of res...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Goldman MJ,Ross IF,Goteiner D

    更新日期:1986-06-01 00:00:00

  • Common problems with statistical aspects of periodontal research papers.

    abstract::Enormous advances have been made over the past decade concerning the proper use of statistical techniques in reporting periodontal research. However, numerous articles still appear which use inappropriate or less than optimal statistical methods. This paper describes the most common problems encountered in a recent re...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Emrich LJ

    更新日期:1990-04-01 00:00:00

  • Effects of hydrogen peroxide on oral carcinogenesis in hamsters.

    abstract::The effects of twice weekly topical applications of hydrogen peroxide on the buccal epithelium of Syrian hamsters were studied. Animals were treated either with hydrogen peroxide alone, with hydrogen peroxide and the carcinogen 9, 10-dimethyl-1,2-benzanthracene (DMBA), or with DMBA alone. In animals treated with 30% H...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Weitzman SA,Weitberg AB,Stossel TP,Schwartz J,Shklar G

    更新日期:1986-11-01 00:00:00

  • The long-term effectiveness of periodontal therapy in a clinical practice.

    abstract::A retrospective study was conducted of 620 patients in a periodontal practice over the years 1960 to 1982. Various periodontal therapies were assessed by using careful office protocols, meticulous record-keeping and well-defined outcome criteria. Four treatment outcome categories were used: two representing satisfacto...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Meador HL,Lane JJ,Suddick RP

    更新日期:1985-05-01 00:00:00

  • The impact of periodontal therapy and the adjunctive effect of antiseptics on breath odor-related outcome variables: a double-blind randomized study.

    abstract:BACKGROUND:Bad breath is often caused by periodontitis and/or tongue coating. This study followed the impact of initial periodontal therapy on several halitosis-related outcome variables over a 6-month period. Organoleptic ratings are often uncomfortable for the patient and have several disadvantages. They are, for ins...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Quirynen M,Zhao H,Soers C,Dekeyser C,Pauwels M,Coucke W,Steenberghe Dv

    更新日期:2005-05-01 00:00:00

  • Periodontal disease and detection of human herpesviruses in saliva and gingival crevicular fluid of chronic kidney disease patients.

    abstract:BACKGROUND:Patients with chronic kidney disease (CKD) have inability to maintain the normal levels of protein metabolism products, blood pressure and hematocrit. Periodontal disease involves an inflammatory destructive process. Identification of opportunistic viruses is extremely important as they are associated with c...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Pallos D,Ruivo GF,Ferrari-Junior SH,Pannuti CS,Perozini C,Sarmento DJS,Palmieri M,Souza ACMF,Tozetto-Mendoza TR,Doglio A,Braz-Silva PH

    更新日期:2020-09-01 00:00:00

  • Levels of interferon-gamma and transcription factor T-bet in progressive periodontal lesions in patients with chronic periodontitis.

    abstract:BACKGROUND:Periodontitis is an infection with an episodic pattern of tissue-support destruction. During the generation of a primary CD4(+) T helper 1 (Th1) response, interferon-gamma (IFN-gamma) acts as a positive regulator by selectively inducing Th1 differentiation through increased transcription of T-bet. The aims o...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Dutzan N,Vernal R,Hernandez M,Dezerega A,Rivera O,Silva N,Aguillon JC,Puente J,Pozo P,Gamonal J

    更新日期:2009-02-01 00:00:00

  • Irritant contact stomatitis: a review of the condition.

    abstract::Several different types of interactions are possible between a chemical, a mixture of chemicals, and associated extrinsic factors (i.e., mechanical irritation) in the oral mucosa. These interactions can be broadly classified as irritative or allergenic in nature. In each case, the pathology usually includes mucosal in...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,评审


    authors: Davis CC,Squier CA,Lilly GE

    更新日期:1998-06-01 00:00:00

  • The etiology of hard- and soft-tissue deficiencies at dental implants: A narrative review.

    abstract:OBJECTIVE:The objective of the present paper was to review factors and conditions that are associated with hard and soft-tissue deficiencies at implant sites. IMPORTANCE:Hard- and soft-tissue deficiencies at dental implants are common clinical findings. They can lead to complications and compromise implant survival an...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,评审


    authors: Hämmerle CHF,Tarnow D

    更新日期:2018-06-01 00:00:00

  • Opposing effects of diabetes and tetracycline on the degradation of collagen membranes in rats.

    abstract:BACKGROUND:Increased collagenolytic activity, characteristic of uncontrolled diabetes, may compromise collagen membrane (CM) survival. Tetracycline (TCN) possesses anticollagenolytic properties and delays CM degradation in healthy animals. This study evaluates the degradation of TCN--immersed and -non-immersed CMs in r...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Eliezer M,Nemcovsky C,Romanos G,Kozlovsky A,Tal H,Kolerman R,Weinreb M,Moses O

    更新日期:2013-04-01 00:00:00

  • Variations in subgingival microflora from healthy and intervention sites using probing depth and bacteriologic identification criteria.

    abstract::This investigation studied the changes in probing depths and subgingival microbial flora of clinically healthy and diseased sites before and after scaling and root planing. Thirty-six sites in 18 individuals were studied in two groups of patients, Group I (9 patients) had received no periodontal surgery, while group I...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Greenwell H 3rd,Bissada NF

    更新日期:1984-07-01 00:00:00

  • Biofilm Lysine Decarboxylase, a New Therapeutic Target for Periodontal Inflammation.

    abstract:BACKGROUND:Lysine, a nutritionally essential amino acid, enters the oral cavity in gingival crevicular fluid (GCF). During oral hygiene restriction (OHR), lysine decarboxylase (LDC) in dento-gingival biofilms converts lysine to cadaverine. Lysine depletion impairs the dental epithelial barrier to bacterial proinflammat...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Lohinai Z,Keremi B,Szöko E,Tábi T,Szabo C,Tulassay Z,DiCesare JC,Davis CA,Collins LM,Levine M

    更新日期:2015-10-01 00:00:00

  • Microstructural volumetric analysis of the jaw following dental implantation under systemic bisphosphonate delivery: An in vivo and ex vivo rat study.

    abstract:BACKGROUND:Because of bisphosphonate medication, dental implantation with a subsequent infection poses a relevant risk factor to suffer from medication-related osteonecrosis of the jaw. This rat study evaluated different implant materials under systemic bisphosphonate delivery using micro-computed tomography (μCT) imag...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Kniha K,Bock A,Peters F,Magnuska ZA,Gremse F,Möhlhenrich SC,Hölzle F,Modabber A

    更新日期:2020-11-30 00:00:00

  • Biomarkers and Bacteria Around Implants and Natural Teeth in the Same Individuals.

    abstract:BACKGROUND:This cross-sectional study assesses cytokine levels in peri-implant crevicular fluid (PICF)/gingival crevicular fluid (GCF) and a selection of subgingival/submucosal plaque bacteria from clinically healthy or diseased sites in the same individuals. METHODS:Samples from 97 implants/teeth (58 implants [19 hea...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Gürlek Ö,Gümüş P,Nile CJ,Lappin DF,Buduneli N

    更新日期:2017-08-01 00:00:00

  • Adsorption of enamel matrix proteins to a bovine-derived bone grafting material and its regulation of cell adhesion, proliferation, and differentiation.

    abstract:BACKGROUND:The use of various combinations of enamel matrix derivative (EMD) and grafting materials has been shown to promote periodontal wound healing/regeneration. However, the downstream cellular behavior of periodontal ligament (PDL) cells and osteoblasts has not yet been studied. Furthermore, it is unknown to what...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Miron RJ,Bosshardt DD,Hedbom E,Zhang Y,Haenni B,Buser D,Sculean A

    更新日期:2012-07-01 00:00:00

  • Clinical oral medicine. An exciting facet of postdoctoral periodontic training.

    abstract::This paper briefly reviews the historical relationship between oral medicine and periodontics. The reasons for including structured clinical oral medicine in a postdoctoral program in periodontics are discussed. Problems and solutions to such an integrated program are outlined. Guidelines for broadening the base of th...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Hurt WC

    更新日期:1986-07-01 00:00:00

  • Oral manifestations of hereditary hemorrhagic telangiectasia (Osler-Weber-Rendu disease). Review and case reports.

    abstract::A review of hereditary hemorrhagic telangiectasia (Osler-Weber-Rendu Disease) is presented. Special emphasis is placed on the oral manifestations which include hemorrhagic vesicles, nodules, papules and ulcers that involve the gingiva and other areas of the mucosa. Two cases are included which were seen in a periodont...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Bartolucci EG,Swan RH,Hurt WC

    更新日期:1982-03-01 00:00:00

  • Aggregatibacter actinomycetemcomitans-Induced AIM2 Inflammasome Activation Is Suppressed by Xylitol in Differentiated THP-1 Macrophages.

    abstract:BACKGROUND:Aggressive periodontitis is characterized by rapid destruction of periodontal tissue caused by Aggregatibacter actinomycetemcomitans. Interleukin (IL)-1β is a proinflammatory cytokine, and its production is tightly regulated by inflammasome activation. Xylitol, an anticaries agent, is anti-inflammatory, but ...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Kim S,Park MH,Song YR,Na HS,Chung J

    更新日期:2016-06-01 00:00:00

  • Depletion of polyamines and increase of transforming growth factor-beta1, c-myc, collagen-type I, matrix metalloproteinase-1, and metalloproteinase-2 mRNA in primary human gingival fibroblasts.

    abstract:BACKGROUND:The polyamines spermidine, spermine, and putrescine are known to be deeply linked with growth processes, gene expression, and extracellular matrix synthesis. Their cellular content depends primarily on the activity of the enzyme ornithine decarboxylase. High levels of ornithine decarboxylase and polyamines h...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Stabellini G,Moscheni C,Gagliano N,Dellavia C,Calastrini C,Ferioli ME,Gioia M

    更新日期:2005-03-01 00:00:00

  • Retention of multi-rooted teeth with class III furcation lesions utilizing resins. Report of 17 cases.

    abstract:BACKGROUND:Treatment of periodontally diseased multi-rooted posterior teeth has conventionally consisted of scaling and root planing, apically positioned flaps, tunneling, root amputation, root resection, guided tissue regeneration, osseous grafting, and combination osseous graft and barrier membrane techniques. Succes...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Anderegg CR,Metzler DG

    更新日期:2000-06-01 00:00:00

  • Healing of the oral mucosa with the use of collagen artificial skin.

    abstract::Wounds were prepared in the oral cavity of 15 rabbits and five dogs, and an enzyme-solubilized calfskin collagen was placed over the surgery sites on one side. The contralateral sides acted as controls. Results indicated that the membrane was biologically acceptable to the oral mucosa of rabbits and dogs. Th collagen ...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Levin MP,Tsaknis PJ,Cutright DE

    更新日期:1979-05-01 00:00:00

  • Exposure to occupational noise and periodontitis in Korean workers.

    abstract:BACKGROUND:Chronic exposure to occupational noise is reported to cause a wide range of health problems. But, there are no reports on studies investigating the association between noise exposure and periodontitis. The purpose of this study was to examine the relationship between occupational noise exposure and periodont...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Shim SH,Han DH

    更新日期:2018-04-01 00:00:00

  • Subgingival plaque removal using a new air-polishing device.

    abstract:BACKGROUND:The purpose of this study is to evaluate the safety, patient acceptance, and short-term microbiologic effect of a new air-polishing device in subjects in maintenance care with residual pockets > or =5 mm. METHODS:This was an examiner-masked, randomized, split-mouth clinical trial. A new disposable nozzle, a...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,随机对照试验


    authors: Moëne R,Décaillet F,Andersen E,Mombelli A

    更新日期:2010-01-01 00:00:00

  • Actinobacillus actinomycetemcomitans lipopolysaccharide-mediated experimental bone loss model for aggressive periodontitis.

    abstract:BACKGROUND:Bacterial constituents, such as Gram-negative derived lipopolysaccharide (LPS), can initiate inflammatory bone loss through induction of host-derived inflammatory cytokines. The aim of this study was to establish a model of aggressive inflammatory alveolar bone loss in rats using LPS derived from the periodo...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Rogers JE,Li F,Coatney DD,Rossa C,Bronson P,Krieder JM,Giannobile WV,Kirkwood KL

    更新日期:2007-03-01 00:00:00

  • Radiographic methods for the detection of progressive alveolar bone loss.

    abstract::Intraoral transmission radiographs have been the primary diagnostic method for the assessment of bone support as well as for the detection and measurement of osseous changes due to periodontitis. The purpose of the present paper is three-fold. The first is to review radiographic techniques for the assessment of period...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,评审


    authors: Jeffcoat MK

    更新日期:1992-04-01 00:00:00

  • The gene expression profile in refractory periodontitis patients.

    abstract:BACKGROUND:There are no specific bacterial profiles or diagnostic tests capable of identifying refractory periodontitis patients before a treatment regimen is initiated. Therefore, in this high-risk cohort of patients who do not respond appropriately, host factors that might be partly under genetic control may play a c...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Kim DM,Ramoni MF,Nevins M,Fiorellini JP

    更新日期:2006-06-01 00:00:00

  • Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: radiographic observations.

    abstract:BACKGROUND:Platelet-rich plasma (PRP) harbors growth factors identified in bone. It has been suggested that these factors enhance osteogenesis. The objective of this study was to conduct a radiographic evaluation on local bone formation following surgical implantation of a PRP preparation using a critical-size rat calv...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Pryor ME,Yang J,Polimeni G,Koo KT,Hartman MJ,Gross H,Agelan A,Manns JM,Wikesjö UM

    更新日期:2005-08-01 00:00:00

  • The healing of gingival wounds in miniature swine.

    abstract::Standard wounds of simple incision and partial excision of the dentogingival junction were made in miniature swine. The healing from the 1st to the 14th day were described clinically and histologically, and the standardization of the wounds permitted quantitative and statistical analysis. In wounds that are closely ad...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Wirthlin MR,Yeager JE,Hancock EB,Gaugler RW

    更新日期:1980-06-01 00:00:00

  • Oral aspects of Gaucher's disease: a literature review and case report.

    abstract:BACKGROUND:Gaucher's disease (GD) is a lysosomal storage disease with a high incidence in Ashkenazi Jews. The disease is caused by an autosomally recessive inherited deficiency of the lysosomal enzyme glucocerebrosidase, leading to the accumulation of glucocerebroside in macrophages. The lipid-laden macrophages, called...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,评审


    authors: Horwitz J,Hirsh I,Machtei EE

    更新日期:2007-04-01 00:00:00

  • Changes of subgingival total colony forming units and black pigmented bacteroides after a single irrigation of periodontal pockets with 1.64% SnF2.

    abstract::Periodontal pockets of 11 subjects were irrigated once with either 1.64% SnF2 or saline applied by syringe. Changes on the total anaerobic colony forming units (CFU) and black pigmented bacteroides (BPBs) of the irrigated pockets were assessed for 31 days. The results showed little change of the CFUs by either single ...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Schmid E,Kornman KS,Tinanoff N

    更新日期:1985-06-01 00:00:00