Abstract:
BACKGROUND:Phosphatidylinositol 3-kinase (PI3K)-AKT signaling is a well-characterized pathway involved in the control of cell proliferation, apoptosis and oncogenesis. LY294002 is a commonly used pharmacologic inhibitor which acts at the ATP-binding site of the PI3K enzyme, thus selectively inhibiting the PI3K-AKT nexus. The purpose of the present study was to examine whether PI3K inhibited by LY294002 had an effect on human bladder cancer cells. METHODS:After treatment with LY294002, MTT assay, chemosensitivity test, colony formation assay, apoptosis assay and Western blot analysis were conducted in EJ cells. RESULT:EJ cells treated with LY294002 showed significant AKT phosphorylation suppression in a dose-response manner. Also, PI3K/AKT signaling inhibitor LY294002 suppressed cell proliferation and enhanced the chemosensitivity of doxorubicin in human bladder cancer EJ cells. Furthermore, LY294002 increased cell apoptosis to doxorubicin. CONCLUSION:The augmentation of doxorubicin with PI3K inhibitor LY294002 may resolve the multidrug resistance of bladder cancer, and this may be a new strategy for achieving tolerance for chemotherapeutic agents in bladder cancer therapy.
journal_name
Urol Intjournal_title
Urologia internationalisauthors
Wu D,Tao J,Xu B,Qing W,Li P,Lu Q,Zhang Wdoi
10.1159/000322849subject
Has Abstractpub_date
2011-01-01 00:00:00pages
105-13issue
1eissn
0042-1138issn
1423-0399pii
000322849journal_volume
87pub_type
杂志文章abstract:INTRODUCTION:We investigated the status of erectile function and urinary continence after radical prostatectomy to investigate a possible relation between them and then determined whether the status of postoperative urinary continence affected erectile function. PATIENTS AND METHODS:Seventy-six patients who had no sym...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000078801
更新日期:2004-01-01 00:00:00
abstract::A retrospective analysis based on Robson's tumor stage classification was performed on 56 patients with renal cell carcinoma who had undergone radical nephrectomy through a lumbar flank approach and 35 who had through a transperitoneal approach. The 5-year survival rates of patients with nephrectomy through the lumbar...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281772
更新日期:1991-01-01 00:00:00
abstract::Clear cell adenocarcinoma arising in the lower urinary tract is unusual. We report a case of clear cell adenocarcinoma of the bladder in a 54-year-old male. Cystoscopic examination revealed a tumour arising from the dome of the urinary bladder. Histologically, the tumour was chiefly composed of tubulocystic and papill...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000071856
更新日期:2003-01-01 00:00:00
abstract::The anterior nutcracker syndrome is defined by the compression of the left renal vein between the aorta and superior mesenteric artery, usually related to the occurrence of hematuria. We report the case of an uncommon complication of the nutcracker syndrome. A 75-year-old woman was referred to our institution for left...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000381270
更新日期:2016-01-01 00:00:00
abstract::Intestinal ischemia is reported to be the most common gastrointestinal complication of renal transplantation and a potential cause of morbidity and mortality. The recent use of more potent immunosuppressive drug regimens has reduced the incidence of acute rejection, increasing the incidence of potentially fatal infect...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000106334
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Silicone has been the standard prosthesis material for the last three decades but new materials are being searched because of the known disadvantages of silicone such as migration and high cost. We wanted to test in rats whether or not polymethylmethacrylate could be an alternative to silicone. MATERIALS ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000075277
更新日期:2004-01-01 00:00:00
abstract::Ectopia of the vas deferens (EVD) combined with agenesis of the seminal vesicle, is a rare congenital abnormality. We describe a case of EVD with agenesis of the ipsilateral seminal vesicle, presenting with pneumaturia and frequent urinary tract infection. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000070144
更新日期:2003-01-01 00:00:00
abstract::Testicular microlithiasis associated with seminoma was found in a 32-year-old infertile man. Gray-scale and color Doppler ultrasound findings are discussed and a brief review of the literature is presented. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282928
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:To analyze positive surgical margins (PSM) after radical prostatectomy (RP) in the overall population and in patients previously treated with transurethral resection of the prostate (TURP). MATERIALS AND METHODS:2,408 patients treated with RP for clinically localized prostate cancer (PCa) were consecutively ...
journal_title:Urologia internationalis
pub_type: 杂志文章,多中心研究
doi:10.1159/000346748
更新日期:2013-01-01 00:00:00
abstract::Multiple oral doses of alpha-difluoromethyl-ornithine (alpha-DFMO), 18-24 g/day for up to 2 months, were administered to 2 patients with invasive and metastatic carcinoma of the bladder and to 3 patients with metastatic renal cancer in an open study. The moderate antigrowth effect of alpha-DFMO in these patients was c...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280403
更新日期:1981-01-01 00:00:00
abstract::The authors improved the Nélaton catheter with a pair of carbon fiber electrodes as previously published and also improved the Foley catheter with a pair of carbon fiber electrodes which are less painful and easier to use. We also have made efforts how to record the vesical electromyogram (EMG) simultaneously with cys...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280979
更新日期:1984-01-01 00:00:00
abstract::In unilateral kidney disease, it has been suggested that the ipsilateral state could be estimated by measuring contralateral kidney size; thus, a contralateral increase should reflect an ipsilateral impairment and vice versa. This could easily be performed using ultrasound. In our experiments with partial obstruction ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282898
更新日期:1996-01-01 00:00:00
abstract:INTRODUCTION:Urethral valves can be of enormous clinical importance. Both the prognosis for an affected fetus and the indication for a prenatal therapeutic intervention depend to a high degree on the accuracy of the prenatal diagnosis. PATIENTS AND METHODS:The sonographic findings and the results of the postnatal diag...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000081586
更新日期:2004-01-01 00:00:00
abstract:INTRODUCTION:Augmentation cystoplasty is an effective approach to the detrusor hyperreflexia which is refractory to conservative treatment. Sporadic data have been published in patients with progressive diseases such as multiple sclerosis (MS). MATERIALS AND METHODS:Augmentation ileocystoplasty (Goodwin 'cup-patch') w...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000067708
更新日期:2003-01-01 00:00:00
abstract::A 50-year-old man with hemophilia A presented with recurrent hematuria due to renal stone disease. He was receiving approximately 50,000 units of recombinant factor (rF) VIII concentrate every year due to hematuria. Between 1996 and 2002, his serum creatinine level increased from 0.7 to 1.2 ng/ml. In an effort to reso...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000100846
更新日期:2007-01-01 00:00:00
abstract::We examined Southern blot analysis of genomic DNAs from 70 patients with sporadic renal cell carcinoma, using the human L-myc oncogene fragment as a hybridization probe. Our purpose was to study the relationship between the restriction fragment length polymorphism (RFLP) of the L-myc and the frequencies of metastasis....
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282261
更新日期:1991-01-01 00:00:00
abstract:INTRODUCTION:Uretero-ileal strictures (UES) following urinary diversion are therapeutically challenging. We compared the efficacy, safety and renal outcome following therapeutic endo-urological techniques (EUTs) and open surgical revision of the anastomosis (SRA) for UES. MATERIAL AND METHODS:We retrospectively analys...
journal_title:Urologia internationalis
pub_type: 杂志文章,多中心研究
doi:10.1159/000475508
更新日期:2018-01-01 00:00:00
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journal_title:Urologia internationalis
pub_type: 杂志文章,多中心研究
doi:10.1159/000144051
更新日期:2008-01-01 00:00:00
abstract::Negotiating a tortuous or obstructed ureter with a wire can be time-consuming and sometimes tiring. The principle of using a slippery floppy wire and then replacing it with a stiff wire through a 5-french open-ended ureteral catheter yields a high success rate. The ureteral catheter can be used for a radiocontrast stu...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282713
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the clinical and demographic findings of children with urolithiasis in eastern Turkey. METHODS:We retrospectively reviewed the medical records of 67 children with urolithiasis for clinical and laboratory data. RESULTS:Mean age at the time of diagnosis was 39.1 +/- 41.2 months. Complaints at admi...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000230027
更新日期:2009-01-01 00:00:00
abstract::Of 211 incontinent women the answer to each item of a questionnaire is correlated with the urodynamical data and the discriminative value for the type of incontinence is calculated. The relevant questions are discussed. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281355
更新日期:1988-01-01 00:00:00
abstract::We present a case of an appendicular mass mimicking symptoms of a bladder tumour in a 27-year-old female. The patient initially presented with a 5-month history of intermittent lower abdominal pain associated with dysuria, anorexia and weight loss. Cystoscopy revealed an erythematous urothelium with the appearance of ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000089178
更新日期:2005-01-01 00:00:00
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journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000370166
更新日期:2015-01-01 00:00:00
abstract::Inguinoscrotal herniation of the ureter is a rare finding with the potential for serious surgical complications. Two anatomic forms are defined. In the more common paraperitoneal variant, the herniating peritoneal sac drags the ureter and sometimes other abdominal structures with it. The uncommon variant--extraperiton...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000224879
更新日期:2009-01-01 00:00:00
abstract::We report 2 patients with epididymo-orchitis, caused by Brucella melitensis, accompanied by severe delay of diagnosis. In 1 patient the correct diagnosis was only suspected after his wife had been diagnosed with brucellosis. Our cases illustrate that diagnosing brucellosis can be difficult in nonendemic areas, especia...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000218542
更新日期:2009-01-01 00:00:00
abstract:INTRODUCTION:Intravesical recurrence after treatment of primary upper urinary tract urothelial carcinomas (UUT-UCs) is common. While intravesical instillation is widely used to prevent recurrence after transurethral resection for primary bladder tumors, there is still no consensus on the prophylactic capability of intr...
journal_title:Urologia internationalis
pub_type: 杂志文章,meta分析,评审
doi:10.1159/000350508
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVES:To cultivate primary human bladder smooth muscle cells (hBSMCs) in vitro and to study the influences of different sustained hydrostatic pressures upon hBSMCs in terms of cell proliferation and expression of gap-junction connexin 43 (Cx43). METHODS:hBSMCs were exposed to 0, 20, 40, 60, 80 and 100 cm H(2)O su...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000338924
更新日期:2013-01-01 00:00:00
abstract::Loin pain haematuria (LPHS) is a rare and difficult-to-diagnose syndrome. Different therapeutic approaches have been used historically with little or no success. We report a case of LPHS in which bilateral renal autotransplantation led to pain relief, cessation of all medication and no recurrence beyond two years of f...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000430503
更新日期:2017-01-01 00:00:00
abstract::Human factors is a definition that includes the science of understanding the properties of human capability, the application of this understanding to the design and development of systems and services, the art of ensuring their successful applications to a program. The field of human factors traces its origins to the ...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000241662
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVES:To introduce our modified retroperitoneoscopic living-donor nephrectomy (RPLDN) techniques and estimate the learning curve. METHODS:We retrospectively evaluated 121 consecutive donors who underwent modified RPLDN performed by a single surgeon. While the surgeon controlled the renal vessels, one hand was ins...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000493765
更新日期:2018-01-01 00:00:00