Abstract:
:Plasminogen activator inhibitor-1 (PAI-1) is the main inhibitor of tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA) and therefore plays an important role in the plasminogen/plasmin system. PAI-1 is involved in a variety of cardiovascular diseases (mainly through inhibition of t-PA) as well as in cell migration and tumor development (mainly through inhibition of u-PA and interaction with vitronectin). PAI-1 is a unique member of the serpin superfamily, exhibiting particular unique conformational and functional properties. Since its involvement in various biological and pathophysiological processes PAI-1 has been the subject of many in vivo studies in mouse models. We briefly discuss structural and physiological differences between human and mouse PAI-1 that should be taken into account prior to extrapolation of data obtained in mouse models to the human situation. The current review provides an overview of the various models, with a focus on cardiovascular disease and cancer, using wild-type mice or genetically modified mice, either deficient in PAI-1 or overexpressing different variants of PAI-1.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Declerck PJ,Gils A,De Taeye Bdoi
10.1016/B978-0-12-386471-0.00005-5subject
Has Abstractpub_date
2011-01-01 00:00:00pages
77-104eissn
0076-6879issn
1557-7988pii
B978-0-12-386471-0.00005-5journal_volume
499pub_type
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