当前位置： SCI文献检索 > NEUROCHEMISTRY INTERNATIONAL期刊下所有文献 > Polarized hemichannel opening of pannexin 1/connexin 43 contributes to dysregulation of transport function in blood-brain barrier endothelial cells.

Polarized hemichannel opening of pannexin 1/connexin 43 contributes to dysregulation of transport function in blood-brain barrier endothelial cells.

Abstract：

:Dysregulation of blood-brain barrier (BBB) transport exacerbates brain damage in acute ischemic stroke. Here, we aimed to investigate the mechanism of this BBB transport dysregulation by studying the localization and function of pannexin (Px) and connexin (Cx) hemichannels in blood-brain barrier endothelial cells of rat (TR-BBB13 cells) and human (hCMEC/D3 cells) under acute ischemic stroke-mimicking oxygen/glucose deprivation (OGD) and extracellular Ca2+ ([Ca2+]e)-free conditions. TR-BBB13 cells showed increased uptake of hemichannel-permeable sulforhodamine 101, and this increase was markedly inhibited by carbenoxolone, a hemichannel inhibitor. Transcripts of Px1 and Cx43 were detected in TR-BBB13 cells and freshly isolated brain microvascular endothelial cells. The basal compartment-to-cell uptake of hemichannel-permeable propidium iodide was selectively enhanced in hCMEC/D3 cells under [Ca2+]e-free conditions in the basal Transwell chamber. Immunohistochemical analysis revealed the predominant localization of Cx43 on the lateral membranes of hCMEC/D3 cells. [3H]Taurine uptake by hCMEC/D3 cells was significantly reduced in the absence of [Ca2+]e. Functional knock-down of Px1 and Cx43 with mimetic peptides significantly inhibited the increase of ATP release from hCMEC/D3 cells under [Ca2+]e-free conditions. These results suggest that polarized Px1/Cx43 hemichannel opening in brain capillary endothelial cells under acute ischemic stroke-mimicking conditions contributes to dysregulation of BBB transport function, resulting in release of intracellular taurine and ATP.

journal_name

Neurochem Int

journal_title

Neurochemistry international

authors

Tachikawa M,Murakami K,Akaogi R,Akanuma SI,Terasaki T,Hosoya KI

doi

10.1016/j.neuint.2019.104600

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

104600

eissn

0197-0186

issn

1872-9754

pii

S0197-0186(19)30370-5

journal_volume

132

pub_type

杂志文章

相关文献

NEUROCHEMISTRY INTERNATIONAL文献大全
  • Neuronal-glial interactions in rats fed a ketogenic diet.

    abstract：:Glucose is the preferred energy substrate for the adult brain. However, during periods of fasting and consumption of a high fat, low carbohydrate (ketogenic) diet, ketone bodies become major brain fuels. The present study was conducted to investigate how the ketogenic diet influences neuronal-glial interactions in ami...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2005.12.037

    authors： Melø TM,Nehlig A,Sonnewald U

    更新日期：2006-05-01 00:00:00

  • Bradykinin inhibition of cyclic AMP accumulation in D384 astrocytoma cells. Evidence against a role of cyclic GMP.

    abstract：:The present studies were performed in order to examine the possible role of cyclic GMP-stimulated phosphodiesterase (cGMP-PDE) activity in the inhibitory action of the inflammatory peptide bradykinin on cyclic AMP (cAMP) accumulation in D384 cells. Bradykinin decreased the forskolin-stimulated cAMP accumulation in the...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(92)90149-l

    authors： Altiok N,Fredholm BB

    更新日期：1992-09-01 00:00:00

  • Extended periods of neural induction and propagation of embryonic stem cell-derived neural progenitors with EGF and FGF2 enhances Lmx1a expression and neurogenic potential.

    abstract：:Neural stem (NS) cells are multipotent cells defined by their capacity to proliferate and differentiate into all neuronal and glial phenotypes. NS cells can be obtained from specific regions of the adult brain, or generated from embryonic stem cells (ESCs). NS cells differentiate into neural progenitor (NP) cells and ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2011.04.002

    authors： Zeng WR,Fabb SR,Haynes JM,Pouton CW

    更新日期：2011-09-01 00:00:00

  • Inhibition of hippocampal LTP by ginkgolide B is mediated by its blocking action on PAF rather than glycine receptors.

    abstract：:Platelet-activating factor (PAF), a biologically active lipid (1-O-alkyl-2-acetyl-sn-glycero-3-phosphoholine), is identified in different regions of brain, including hippocampus. Specific PAF-activated receptors (PAFRs) are expressed in corresponding brain areas. PAF has been proposed to be a retrograde messenger of l...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(03)00126-8

    authors： Kondratskaya EL,Pankratov YV,Lalo UV,Chatterjee SS,Krishtal OA

    更新日期：2004-02-01 00:00:00

  • Alteration of ethanolamine glycerophospholipid turnover in trembler dysmyelinating mutant: An analysis of the sciatic nerve by biochemistry and radioautography.

    abstract：:The turnover of phospholipids was compared in peripheral nerves of Trembler dysmelinating mutant and control mice, after intraperitoneal and local injection of labeled ethanolamine. In the mutant sciatic nerve, neurochemical analysis showed that [(14)C]ethanolamine is incorporated into EGP (ethanolamine glycerophospho...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(90)90045-u

    authors： Do Thi NA,Bourre JM,Koenig HL,Trouillet V,Dumont O,Piciotti M

    更新日期：1990-01-01 00:00:00

  • Immunocytochemical demonstration of taurine in the retina and pineal organ of the pigeon.

    abstract：:Taurine-like immunoreactivity (TLI) is found in the pigeon retina at high levels within cones, in select cells of the inner nuclear layer and in sublaminae of the inner plexiform layer. At the ultrastructural level a high density of immuno-gold particles in visualized in the ellipsoid, but not in the paraboloid area o...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(95)00043-8

    authors： Ueck M,Hach A,Verdone-Smith C,Lake N

    更新日期：1995-12-01 00:00:00

  • Cholesterol deficiency increases the vulnerability of hippocampal glia in primary culture to glutamate-induced excitotoxicity.

    abstract：:Cholesterol, a molecule critical for cellular function, is found in particular high concentration in the brain and has been implicated to synaptic plasticity and neuronal regeneration. This study was undertaken to investigate the mechanism by which cholesterol shortage modulates glutamate (Glu)-induced excitotoxicity ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(03)00003-2

    authors： Chou YC,Lin SB,Tsai LH,Tsai HI,Lin CM

    更新日期：2003-08-01 00:00:00

  • Systemic pregabalin attenuates facial hypersensitivity and noxious stimulus-evoked release of glutamate in medullary dorsal horn in a rodent model of trigeminal neuropathic pain.

    abstract：:Pregabalin is effective in treating many neuropathic pain conditions. However, the mechanisms of its analgesic effects remain poorly understood. The aim of the present study was to determine whether pregabalin suppresses facial mechanical hypersensitivity and evoked glutamate release in the medullary dorsal horn (MDH)...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2013.02.022

    authors： Kumar N,Cherkas PS,Varathan V,Miyamoto M,Chiang CY,Dostrovsky JO,Sessle BJ,Coderre TJ

    更新日期：2013-05-01 00:00:00

  • Enhancement of serotonin transporter function by tumor necrosis factor alpha but not by interleukin-6.

    abstract：:Serotonin (5-HT) is a prime candidate for studies of the interaction between the nervous and immune systems, since it is both an important neurotransmitter and released at high concentrations at sites of inflammation. Serotonergic neurotransmission is regulated by the 5-HT transporter (5-HTT), which determines the mag...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(98)00026-6

    authors： Mössner R,Heils A,Stöber G,Okladnova O,Daniel S,Lesch KP

    更新日期：1998-09-01 00:00:00

  • Analysis of the role of central and peripheral alpha2-adrenoceptor subtypes in gastric mucosal defense in the rat.

    abstract：:The present study confirmed our previous assumption on the crucial role of central alpha2B-like adrenoceptor subtype in gastric mucosal defense. It was found that beside clonidine, rilmenidine, an alpha2/imidazoline receptor agonist and ST-91, an alpha2B-adrenoceptor preferring agonist inhibited the mucosal lesions in...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2007.07.001

    authors： Gyires K,Zádori ZS,Shujaa N,Minorics R,Falkay G,Mátyus P

    更新日期：2007-10-01 00:00:00

  • 25-Hydroxycholesterol causes death but does not prevent nerve growth factor-induced neurite outgrowth in PC12 cells.

    abstract：:The effects of cholesterol oxides on regular and neuronal PC12 cells were examined. Among the cholesterol oxides tested, 7-beta-, 22(R)- and 25-OH-cholesterol were very toxic to regular PC12 cells such that approximately 80% of cells were killed after a 3 day incubation with 20 micrograms/ml (50 microM) of these agent...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(97)00020-x

    authors： Chang JY,Liu LZ

    更新日期：1997-10-01 00:00:00

  • The GABA(a) receptor complex in the developing chick optic tectum: Ontogeny of [(3)H]muscimol, [(3)H]flunitrazepam and [(35)S]TBPS binding sites.

    abstract：:The developmental profiles of the neurotransmitter recognition site, labeled by [(3)H]muscimol, and of the two main modulatory sites, labeled by [(3)H]flunitrazepam and [ (35)S ]t- butylbicyclophosphorothionate , respectively, within the GABA(A) receptor complex, have been determined in chick tectal lobes between embr...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(87)90032-5

    authors： Batuecas A,Cubero A,Barat A,Ramírez G

    更新日期：1987-01-01 00:00:00

  • Acylation of myelin proteolipid protein in subcellular fractions of rat brainstem.

    abstract：:The acylation of myelin proteolipid protein (PLP) and intermediate protein (IP) was investigated in an in vitro system of tissue slices prepared from actively myelinating rat brainstem. The incorporation of [(3)H]palmitate into the proteins in nine subcellular fractions including myelin and other cellular membranes wh...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(86)90148-8

    authors： Konat G,Gantt G,Hogan EL

    更新日期：1986-01-01 00:00:00

  • Poloxamer 188 rescues MPTP-induced lysosomal membrane integrity impairment in cellular and mouse models of Parkinson's disease.

    abstract：:Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc). Rupture of lysosome is a major cellular stress condition leading to cell death in PD. We have previously shown that environmenta...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2019.03.013

    authors： Dong H,Qin Y,Huang Y,Ji D,Wu F

    更新日期：2019-06-01 00:00:00

  • Contribution of astrocytes to synaptic transmission in the rat supraoptic nucleus.

    abstract：:Astrocytes, besides supporting metabolic and scaffolding functions, play a prominent role in the modulation of neuronal communication. In particular, they are responsible for clearing synaptically-released glutamate via highly specific transporters located on their plasma membrane. Since glutamate is the main excitato...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2003.07.005

    authors： Piet R,Poulain DA,Oliet SH

    更新日期：2004-07-01 00:00:00

  • Parecoxib and its metabolite valdecoxib directly interact with cannabinoid binding sites in CB1-expressing HEK 293 cells and rat brain tissue.

    abstract：:Cyclooxygenase 2 inhibitors (COX 2) such as parecoxib (par) and valdecoxib (val) are used in the treatment of neuropathic pain. Using the radioligand binding assay it was demonstrated that both the prodrug par as well as its active metabolite val have a specific affinity to the cannabinoid (CB) receptor measured in CB...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2010.10.018

    authors： Schröder H,Höllt V,Becker A

    更新日期：2011-01-01 00:00:00

  • Neurons and astrocytes in an infantile neuroaxonal dystrophy (INAD) mouse model show characteristic alterations in glutamate-induced Ca2+ signaling.

    abstract：:INAD (infantile neuroaxonal dystrophy, OMIM#256600), an autosomal recessive inherited degenerative disease, is associated with PLA2G6 mutations. PLA2G6 encodes Ca2+-independent phospholipase A2 (VIA iPLA2). However, it is unclear how the PLA2G6-mutations lead to disease. Non-canonical functions, which were suggested f...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2017.03.004

    authors： Strokin M,Reiser G

    更新日期：2017-09-01 00:00:00

  • Effects of lithium, alone or associated with pilocarpine, on muscarinic and dopaminergic receptors and on phosphoinositide metabolism in rat hippocampus and striatum.

    abstract：:The mechanism of action of lithium (Li) alone or with pilocarpine (Pilo), focusing on muscarinic and dopaminergic systems and also on phosphoinositide metabolism was studied. Li (3 mEq/kg) administered to rats once (1 d) or daily for 7 days (7 d), 24 h before Pilo (15 mg/kg), exacerbated cholinergic signs, leading to ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(98)00028-x

    authors： Marinho MM,de Sousa FC,de Bruin VM,Vale MR,Viana GS

    更新日期：1998-10-01 00:00:00

  • Diet-induced ketosis increases monocarboxylate transporter (MCT1) levels in rat brain.

    abstract：:Monocarboxylate transporter (MCT1) levels in brains of adult Long-Evans rats on a high-fat (ketogenic) diet were investigated using light and electron microscopic immunocytochemical methods. Rats given the ketogenic diet (91% fat and 9% protein) for up to 6 weeks had increased levels of the monocarboxylate transporter...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/s0197-0186(00)00102-9

    authors： Leino RL,Gerhart DZ,Duelli R,Enerson BE,Drewes LR

    更新日期：2001-05-01 00:00:00

  • Neurohormetic phytochemicals: An evolutionary-bioenergetic perspective.

    abstract：:The impact of dietary factors on brain health and vulnerability to disease is increasingly appreciated. The results of epidemiological studies, and intervention trials in animal models suggest that diets rich in phytochemicals can enhance neuroplasticity and resistance to neurodegeneration. Here we describe how intera...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2015.03.009

    authors： Murugaiyah V,Mattson MP

    更新日期：2015-10-01 00:00:00

  • Recruitment of peripheral-type benzodiazepine receptors after acute stress in chick forebrain membranes: action of Triton X-100.

    abstract：:A significant increase in the number of measurable [3H]Ro 5-4864 receptors was found in forebrain membranes of chicks submitted to 15 min of acute swim stress compared to non-stressed chicks. In addition, low subsolubilizing concentrations of Triton X-100 caused a significant increase in the measurable [3H]Ro 5-4864 r...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(95)00093-3

    authors： Marín RH,Arce A,Martijena ID

    更新日期：1996-04-01 00:00:00

  • Revisiting the neuropathogenesis of Zellweger syndrome.

    abstract：:Zellweger syndrome (ZS) is a neonatal-lethal genetic disease that affects all tissues, and features neuropathology that involves primary developmental defects as well as neurodegeneration. Neuropathological changes include abnormal neuronal migration affecting the cerebral hemispheres, cerebellum and inferior olivary ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuint.2014.02.007

    authors： Crane DI

    更新日期：2014-04-01 00:00:00

  • External pH changes affect NMDA-evoked and spontaneous release of cholecystokinin, somatostatin and noradrenaline from rat cerebrocortical nerve endings.

    abstract：:It was previously reported that the K+-evoked release of somatostatin-like immunoreactivity (SRIF-LI) and of cholecystokinin-like immunoreactivity (CCK-LI) from superfused rat cerebrocortical synaptosomes can be enhanced by NMDA or D-serine alone. We here studied the effects of extraterminal pH changes on SRIF-LI and ...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2004.03.005

    authors： Gemignani A,Paudice P,Longordo F,Raiteri M

    更新日期：2004-10-01 00:00:00

  • Slow anterograde axonal transport of calpain I and II.

    abstract：:Following intravitreal injections of (35)S-amino acids to label axonally transported proteins, calpain I and II from the superior colliculi and the lateral geniculate bodies were isolated via hydrophobic interaction chromatography, anion exchange HPLC and sodium-dodecyl sulphate electrophoresis. Among the slowly trans...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/0197-0186(90)90032-o

    authors： Nilsson E,Karlsson JO

    更新日期：1990-01-01 00:00:00

  • Calcium regulates the mode of exocytosis induced by hypotonic shock in isolated neuronal presynaptic endings.

    abstract：:A decrease in the osmolarity of incubation medium is accompanied by calcium influx in neuronal presynaptic endings. We studied the influence of Ca2+ on exocytosis induced by hypotonic shock using the hydrophilic fluorescent dye acridine orange and the hydrophobic fluorescent dye FM2-10. It was shown using acridine ora...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2004.09.002

    authors： Waseem TV,Rakovich AA,Lavrukevich TV,Konev SV,Fedorovich SV

    更新日期：2005-02-01 00:00:00

  • Peripheral iron dextran induced degeneration of dopaminergic neurons in rat substantia nigra.

    abstract：:Iron accumulation is considered to be involved in the pathogenesis of Parkinson's disease. To demonstrate the relationship between peripheral iron overload and dopaminergic neuron loss in rat substantia nigra (SN), in the present study we used fast cyclic voltammetry, tyrosine hydroxylase (TH) immunohistochemistry, Pe...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2007.03.006

    authors： Jiang H,Song N,Wang J,Ren LY,Xie JX

    更新日期：2007-07-01 00:00:00

  • Expression of lysosomal protective protein/cathepsin A in a stably transformed human neuroblastoma cell line during bi-directional differentiation into neuronal and Schwannian cells.

    abstract：:Human neuroblastoma GOTO cell lines were established that stably express recombinant human lysosomal protective protein/cathepsin A (PPCA) cDNA by transfection. Intracellular cathepsin A (acid serine carboxypeptidase) activity increased four-fold compared with in those of the parent and mock-transfected cell lines. Th...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2003.07.001

    authors： Itoh K,Satoh Y,Kadota Y,Oheda Y,Kuwahara J,Shimmoto M,Sakuraba H

    更新日期：2004-05-01 00:00:00

  • Long-term kainic acid exposure reveals compartmentation of glutamate and glutamine metabolism in cultured cerebellar neurons.

    abstract：:Glutamate neurotoxicity is implicated in most neurodegenerative diseases, and in the present study the long-term effects of the glutamate agonist kainic acid (KA) on cerebellar neurons are investigated. Primary cell cultures, mainly consisting of glutamatergic granule neurons, were cultured in medium containing 0.05 o...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2006.11.004

    authors： Olstad E,Qu H,Sonnewald U

    更新日期：2007-06-01 00:00:00

  • An interaction between benzodiazepines and neuroactive steroids at GABA A receptors in cultured hippocampal neurons.

    abstract：:Neurosteroids are modulators of several receptors and ion channels and are implicated in the pathophysiology of several neuropsychiatric diseases including hepatic encephalopathy (HE). The neurosteroid, allopregnanolone, a positive allosteric modulator of GABA(A) receptors, accumulates in the brains of HE patients whe...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2005.12.006

    authors： Ahboucha S,Coyne L,Hirakawa R,Butterworth RF,Halliwell RF

    更新日期：2006-06-01 00:00:00

  • Zinc transporter 3 immunohistochemical tracing of sprouting mossy fibres.

    abstract：:Zinc transporter 3 (ZNT3) has been shown to transport zinc ions from the cytosol into presynaptic vesicles in the mammalian brain. Several studies have stated that the zinc ion containing synaptic vesicles of zinc-enriched neurons (ZEN) are loaded with ZNT3 proteins in their membranes. This fact makes it possible to t...

    journal_title：Neurochemistry international

    pub_type： 杂志文章

    doi：10.1016/j.neuint.2008.02.008

    authors： Chi ZH,Wang X,Cai JQ,Stoltenberg M,Danscher G,Wang ZY

    更新日期：2008-06-01 00:00:00