Optimal specimen processing of fine needle aspirates of non-Hodgkin lymphoma.

Abstract:

UNLABELLED:Fine needle aspiration (FNA) in conjunction with flow cytometry (FC) is a useful technique for non-Hodgkin's lymphoma (NHL) diagnosis. We sought to investigate the effect of storage medium and time to processing on lymph node (LN) FNA viability. Benign LN FNAs were distributed among Roswell Park Memorial Institute (RPMI), Hanks' Balanced Salt Solution (HBSS) and Dulbecco's Modified Eagle's Medium (DMEM) storage media, and viability was compared at 0, 4.5 and 24 hours. FC survival analysis showed viable cells (%): at 0 hrs: HBSS 83.6%, RPMI 87.7%, DMEM 87.7%. At 4.5 hrs: HBSS 86.3%, RPMI 89.0%, DMEM 78.2%. At 24 hrs: HBSS 82.7%, RPMI 86.7%, DMEM 77.2%. FNA from a peri-pancreatic LN involved by grade 2 follicular lymphoma was stored in RPMI at 4° C and analyzed at 1, 3, 5 and 7 days. Over 90% of follicular lymphoma cells were suitable for FC analysis at 1, 3, and 5 days after collection, decreasing to 76% at 7 days. IN CONCLUSION:RPMI appears to be the optimal storage medium compared to DMEM and HBSS.An NHL FNA sample stored at 4° C remains suitable for FC analysis for up to 5 days.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Shetuni B,Lakey M,Kulesza P

doi

10.1002/dc.21780

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

984-6

issue

11

eissn

8755-1039

issn

1097-0339

journal_volume

40

pub_type

杂志文章
  • Atypical squamous cells of undetermined significance: A cytohistological study in a colposcopy clinic.

    abstract::Cytohistologic correlation was performed by 3 observers on 100 atypical squamous cells of undetermined significance (ASCUS) cases from a colposcopy clinic. Our objectives were to: 1) subclassify ASCUS cases and determine their clinical significance; 2) assess the independent predictive value of different cytologic par...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199909)21:3<211::aid-dc14>

    authors: Ettler HC,Joseph MG,Downing PA,Suskin NG,Wright VC

    更新日期:1999-09-01 00:00:00

  • Image quantification technology of the heterochromatin and euchromatin region for differential diagnosis in the lobular endocervical glandular hyperplasia.

    abstract:BACKGROUND:Lobular endocervical glandular hyperplasia (LEGH) was first described by Nucci et al. in 1999 and is believed to be a precancerous lesion of minimal deviation adenocarcinoma and gastric-type adenocarcinoma in the uterine cervix. LEGH lesions do not always exhibit apparent cellular and structural atypia, so a...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24155

    authors: Kimura F,Kobayashi T,Kanai R,Kobayashi Y,Yuhi O,Ota H,Yamaguchi M,Yokokawa Y,Uehara T,Ishii K

    更新日期:2019-06-01 00:00:00

  • Use of ThinPrep monolayer technique and cytospin preparation in urine cytology: a comparative analysis.

    abstract::We compared the ThinPrep (TP) technique to the cytospin (CS) preparation in the cytological diagnosis of urine by processing 79 specimens by these two techniques. Ten cases were positive for malignancy (six high grade (HG)/carcinoma in situ; four low grade (LG) transitional cell carcinomas (TCC)). Forty-eight cases we...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10245

    authors: Nassar H,Ali-Fehmi R,Madan S

    更新日期:2003-03-01 00:00:00

  • Nuclear morphometry and texture analysis of B-cell non-Hodgkin lymphoma: utility in subclassification on cytosmears.

    abstract::Non-Hodgkin lymphoma (NHL) is a heterogeneous group of lymphoid neoplasms and accurate subclassification is an essential prerequisite for proper management of patients. This study was aimed at evaluating the utility of nuclear morphometry and textural features on cytology smears to classify the cases of NHL on aspirat...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21154

    authors: Gupta S,Gupta R,Singh S,Gupta K,Kudesia M

    更新日期:2010-02-01 00:00:00

  • Diagnostic significance and possible pitfalls of nuclear grooves in extrathyroid cytology.

    abstract::The diagnostic significance of grooved nuclei in the cytology of extrathyroid lesions is not well-described in the literature. Isolated cases of metastatic adult granulosa cell tumor, reactive mesothelial hyperplasia, Langerhans' cell histiocytosis, recurrent renal cell carcinoma, and round cell liposarcoma were revie...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199701)16:1<57::aid-dc14>3

    authors: Ng WK,Collins RJ

    更新日期:1997-01-01 00:00:00

  • Pitfalls in the diagnosis of hepatic epithelioid hemangioendothelioma by FNA and needle core biopsy.

    abstract::Epithelioid hemangioendothelioma (EHE) of the liver is an extremely rare tumor that masquerades as an epithelial neoplasm and poses significant diagnostic pitfalls for the cytopathologist. This report describes a 64-year-old woman whom on computerized tomography was found to have multiple peripherally calcified hypode...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22943

    authors: Jurczyk M,Zhu B,Laskin W,Lin X

    更新日期:2014-06-01 00:00:00

  • Hepatic angiomyolipoma with predominant epithelioid component: Diagnostic clues on aspiration and core needle biopsies.

    abstract::Hepatic angiomyolipoma (HAML) is a rare mesenchymal neoplasm that belongs to the perivascular epithelioid tumor family. Though it is characteristically, a triphasic tumor composed of smooth muscle, blood vessels, and adipocytes, the smooth muscle cells are often epithelioid and can represent the near-entirety of the t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24688

    authors: De la Sancha C,Khan S,Alruwaii F,Cramer H,Saxena R

    更新日期:2020-12-29 00:00:00

  • Chest wall abscess: an unusual presentation of Hodgkin's lymphoma.

    abstract::A chest wall abscess is a very rare presentation of extranodal Hodgkin's lymphoma (HL); only one case has been reported to date. Here, we describe a case of a 38-yr-old man with HL whose initial presentation was a chest wall abscess. The diagnosis of HL was suggested by cytological examination of the purulent discharg...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20220

    authors: Khalbuss WE,Grigorian S,Mignone J,D'Agostino HJ

    更新日期:2005-07-01 00:00:00

  • Gouty tophus presenting as a soft-tissue mass diagnosed by fine-needle aspiration: a case report.

    abstract::We present a case in which a primary diagnosis of gout was made by fine-needle aspiration. The patient had a right distal ulnar mass, and the initial clinical and radiological diagnoses were that of giant cell tumor of tendon sheath. While tophi are the hallmark of gout, they rarely undergo aspiration because of the k...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0339(199609)15:3<246::AID-DC14>

    authors: Liu K,Moffatt EJ,Hudson ER,Layfield LJ

    更新日期:1996-09-01 00:00:00

  • Ovarian carcinoma cells in effusions show increased S-phase fraction compared to corresponding primary tumors.

    abstract::The objective of this study was to analyze large-scale genomic patterns during disease progression from primary tumor to effusion in ovarian carcinoma, and to study the association between DNA ploidy parameters in effusions, proliferation/survival markers, and clinicopathologic characteristics. DNA ploidy status, DNA ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20879

    authors: Kleinberg L,Pradhan M,Tropé CG,Nesland JM,Davidson B,Risberg B

    更新日期:2008-09-01 00:00:00

  • Tracheal aspirate cytology and bronchopulmonary dysplasia.

    abstract::Sequential tracheal aspirates from 39 neonates with hyaline membrane disease were examined to correlate cytological findings with the development of bronchopulmonary dysplasia (BPD). A total of 224 tracheal aspirates were examined from these infants, 15 of whom developed BPD as diagnosed by conventional clinical and r...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840020307

    authors: Rothberg AD,Miot A,Leiman G

    更新日期:1986-09-01 00:00:00

  • Fine-needle aspiration cytology in locally advanced breast adenocarcinoma: a case with complete response to preoperative chemotherapy in association with granulomatous inflammatory reaction.

    abstract::Preoperative chemotherapy for locally advanced breast carcinoma (stage IIIA,B) is increasingly utilized demonstrating a 70 to 95% objective response and 15 to 35% complete response. A 70-yr-old woman presented with a 9 cm left upper outer quadrant breast mass associated with overlying skin redness and a 2.5 cm left ax...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840100413

    authors: Costa MJ,Stewart G,Perez EA,Goodnight JE

    更新日期:1994-01-01 00:00:00

  • Fine-needle aspiration cytology of lipoblastoma: a report of two cases.

    abstract::Lipoblastoma is an uncommon lipomatous tumor that typically occurs in infants and children. It may present as a single subcutaneous nodule or with multiple lesions (lipoblastomatosis). We describe fine-needle aspiration (FNA) cytologic features of two cases that presented as a subcutaneous lump in the scapular area an...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.20073

    authors: López-Ferrer P,Jiménez-Heffernan JA,Yébenes L,Vicandi B,Viguer JM

    更新日期:2005-01-01 00:00:00

  • Flow cytometric analysis of fine needle aspirates is affected by tumor subtype, but not by anatomic location nor technique.

    abstract:BACKGROUND:Current clinical practices are shifting towards utilizing less invasive biopsy techniques, including fine needle aspiration (FNA) and needle core biopsies. If a patient has a suspected hematologic malignancy, a portion of the FNA sample is typically submitted for flow cytometry (FC) analysis, providing valua...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24417

    authors: Wake LM,VandenBussche CJ,Ali SZ,Wagner-Johnston ND,Burns KH,Gocke CD,Vuica-Ross M,Borowitz MJ,Duffield AS

    更新日期:2020-06-01 00:00:00

  • Aspiration cytology of chromophobe cell carcinoma of the kidney.

    abstract::Fine-needle aspiration biopsy findings in three cases of chromophobe cell carcinoma are described and correlated with histologic and ultrastructural observations. In addition, comparisons are made with three cases each of oncocytoma and granular cell carcinoma. The cells in aspiration smears from chromophobe cell carc...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840130403

    authors: Akhtar M,Ali MA

    更新日期:1995-11-01 00:00:00

  • Cytopathological features of solitary fibrous tumor of the pleura: a study of 5 cases.

    abstract::Solitary fibrous tumor is a rare lesion of serosal membranes. In a pleural location, it may be confused clinically with a localized mesothelioma or a peripheral lung tumor. A transthoracic needle aspiration can be done to obtain material for a diagnosis in these easily accessible tumors. In the literature, there are o...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199802)18:2<118::aid-dc7>3

    authors: Weynand B,Collard P,Galant C

    更新日期:1998-02-01 00:00:00

  • The utility of ancillary studies in pediatric FNA cytology.

    abstract::We evaluated the diagnostic contribution of adjunct studies performed on aspirated material in the work-up of pediatric fine-needle aspiration (FNA) biopsies. Ancillary studies were performed on 54 of 136 (39.7%) pediatric FNA biopsies during a 5-year period. In 23 (16.9%) cases, immunocytochemical (ICC) studies, cons...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840080210

    authors: Gurley AM,Silverman JF,Lassaletta MM,Wiley JE,Holbrook CT,Joshi VV

    更新日期:1992-01-01 00:00:00

  • HBME-1 and CK19 are highly discriminatory in the cytological diagnosis of papillary thyroid carcinoma.

    abstract::The cytologic diagnosis of papillary thyroid carcinoma is straightforward in most instances. However, there are some mimics including goitrous nodules and Hurthle cell neoplasms. Many studies have shown the combination of HBME-1 and CK19 expression to be useful in reaching a correct histologic diagnosis on tissue sect...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20841

    authors: Nga ME,Lim GS,Soh CH,Kumarasinghe MP

    更新日期:2008-08-01 00:00:00

  • Interpretation of endometrium obtained by the Endo-pap sampler and a clinical study of its use.

    abstract::Endometrical cytology sampling using the Endo-pap sampler is an inexpensive, painless, and simple procedure. Tissue microfragments obtained include surface epithelial sheets, gland segments, and stromal cell aggregates. Gland segments are critical for evaluation of the sample. Glands are dilated in hyperplasia and the...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840010104

    authors: Palermo VG

    更新日期:1985-01-01 00:00:00

  • Urothelial toxicity following conditioning therapy in bone marrow transplantation and bladder cancer: morphologic and morphometric comparison by exfoliative urinary cytology.

    abstract::Since cyclophosphamide and busulphan used for therapy of bone marrow transplantation (BMT) can cause urothelial cell changes similar to those found in bladder cancer, comparative morphologic and morphometric urinary cytologic research was carried out, examining 812 urine samples taken from 121 patients undergoing BMT ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840080306

    authors: Stella F,Battistelli S,Marcheggiani F,De Santis M,Donnorso RP,Baronciani D,Durazzi SM,Troccoli R,Lucarelli G

    更新日期:1992-01-01 00:00:00

  • Diagnostic value of fine-needle aspiration in supraclavicular lymphadenopathy: a study of 106 patients and review of literature.

    abstract::The simplicity and safety of the fine-needle aspiration (FNA) make it a useful procedure for the initial evaluation of supraclavicular lymphadenopathy. We analyzed data (106 patients) in combination with the data of three recently published studies (total 301 patients) to make the observations more meaningful. The use...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.10002

    authors: Nasuti JF,Mehrotra R,Gupta PK

    更新日期:2001-12-01 00:00:00

  • Infarcted intraduct papilloma diagnosed by fine-needle biopsy: a cytologic, clinical, and mammographic pitfall.

    abstract::Necrosis is a worrisome cytologic feature in breast aspirates which, when paired with suspicious clinical and mammographic findings, could potentially result in a false positive diagnosis. A 66-yr-old female presented with clinical evidence of stage 1 breast carcinoma supported by the mammographic and ultrasound findi...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840110216

    authors: Greenberg ML,Middleton PD,Bilous AM

    更新日期:1994-01-01 00:00:00

  • Extranodal NK/T-cell lymphoma, nasal type of the uterine cervix: A case report.

    abstract::We report a rare case of extranodal NK/T-cell lymphoma, nasal type of the uterine cervix that showed cytologic features mimicking cervical cancer. A 65-year-old woman presented with vaginal bleeding. Gynecological examination revealed a bulky tumor of the cervix. A conventional Papanicolaou-stained cervical smear show...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23439

    authors: Omori M,Oishi N,Nakazawa T,Nakazawa K,Mitsumori T,Yuminamochi T,Kirito K,Hirata S

    更新日期:2016-05-01 00:00:00

  • Detection of tumor cells of serous tubal intraepithelial carcinoma (STIC) in cervical smears and rapid development of the ovarian involvement: A case report.

    abstract::Serous tubal intraepithelial carcinoma (STIC) has attracted attention as a precursor lesion of high-grade serous carcinoma (HGSC) of the ovary. We report the rare case of a woman in whom adenocarcinoma cells were detected in cervical smears and demonstrated to be derived from STIC in the fimbria. The patient was a 48-...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23957

    authors: Shintaku M,Taniguchi H,Yamamoto Y,Kono F,Sumitomo M

    更新日期:2018-11-01 00:00:00

  • Diagnosis of systemic lupus erythematosus in an elderly male by pericardial fluid cytology: a case report.

    abstract::Although pleural and pericardial effusions are common and may rarely be the initial manifestation of disease, there are few reports of SLE diagnosed in a cytopathology laboratory. We describe an unusual case of SLE in a 75-year-old man who presented with cardiac tamponade. Cytologic examination of the pericardial flui...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199805)18:5<346::aid-dc8>3

    authors: Fazio J,Friedman HD,Swerdlow J,Michiel RR

    更新日期:1998-05-01 00:00:00

  • Pregnancy-associated anaplastic large-cell lymphoma of the breast: a rare mimic of ductal carcinoma.

    abstract::Anaplastic large-cell lymphoma (ALCL) is a rare T-cell lymphoma typically seen in children and young adults. It has been described in numerous sites; however, the breast is one of the least common locations. We herein report a case of ALCL arising in the breast of a 36-yr-old pregnant woman. To our knowledge this is t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20442

    authors: Iyengar P,Reid-Nicholson M,Moreira AL

    更新日期:2006-04-01 00:00:00

  • Leiomyosarcoma metastatic to the orbit: diagnosis of fine-needle aspiration.

    abstract::A 47-yr-old man with history of metastatic low-grade rectal leiomyosarcoma presented with progressive protrusion of his left eye due to an enlarging orbital mass. The differential diagnosis included tumor metastasis or orbital infection due to an unknown infectious agent. Diagnostic fine-needle aspiration (FNA) of the...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199711)17:5<369::aid-dc10>

    authors: Logrono R,Inhorn SL,Dortzbach RK,Kurtycz DF

    更新日期:1997-11-01 00:00:00

  • Cytological diagnosis of osteoblastoma of cervical spine: a case report with review of literature.

    abstract::Osteoblastomas are rare benign but sometimes aggressive primary bone tumors (1%), usually seen in males in the 2nd decade. Osteoblastomas, occurring in the spine, constitute a medical emergency due to impending cord compression. Hence a rapid and simple diagnostic modality, such as fine-needle aspiration (FNA) plays a...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23175

    authors: Venugopal SB,Prasad S

    更新日期:2015-03-01 00:00:00

  • Clear cell hepatocellular carcinoma diagnosed by bile duct brushing cytology.

    abstract::Clear cell hepatocellular carcinoma (CCHCC) is an uncommon morphologic variant of HCC and rarely invades into the main bile ducts. Here we describe a case of CCHCC that was diagnosed by bile duct brushing cytology. Liquid-based preparation of brushing specimen showed clusters of atypical epithelial cells with abundant...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23397

    authors: Fu LY,Mitchell KA,Cai G

    更新日期:2016-02-01 00:00:00

  • Cytopathologic characteristics and differential diagnostic considerations of neuroglial heterotopia of the retropharyngeal space.

    abstract::Neuroglial heterotopias (NGH) are rare congenital head and neck lesions composed of differentiated neuroectodermal tissue and representing developmental heterotopias rather than true neoplasms. The case of a male neonate with respiratory distress and early feeding problems depicting a retropharyngeal space mass which ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21575

    authors: Hayashi T,Haba R,Kushida Y,Kadota K,Katsuki N,Bando K,Miyai Y,Shibuya S,Matsunaga T

    更新日期:2011-11-01 00:00:00