Abstract:
UNLABELLED:Fine needle aspiration (FNA) in conjunction with flow cytometry (FC) is a useful technique for non-Hodgkin's lymphoma (NHL) diagnosis. We sought to investigate the effect of storage medium and time to processing on lymph node (LN) FNA viability. Benign LN FNAs were distributed among Roswell Park Memorial Institute (RPMI), Hanks' Balanced Salt Solution (HBSS) and Dulbecco's Modified Eagle's Medium (DMEM) storage media, and viability was compared at 0, 4.5 and 24 hours. FC survival analysis showed viable cells (%): at 0 hrs: HBSS 83.6%, RPMI 87.7%, DMEM 87.7%. At 4.5 hrs: HBSS 86.3%, RPMI 89.0%, DMEM 78.2%. At 24 hrs: HBSS 82.7%, RPMI 86.7%, DMEM 77.2%. FNA from a peri-pancreatic LN involved by grade 2 follicular lymphoma was stored in RPMI at 4° C and analyzed at 1, 3, 5 and 7 days. Over 90% of follicular lymphoma cells were suitable for FC analysis at 1, 3, and 5 days after collection, decreasing to 76% at 7 days. IN CONCLUSION:RPMI appears to be the optimal storage medium compared to DMEM and HBSS.An NHL FNA sample stored at 4° C remains suitable for FC analysis for up to 5 days.
journal_name
Diagn Cytopatholjournal_title
Diagnostic cytopathologyauthors
Shetuni B,Lakey M,Kulesza Pdoi
10.1002/dc.21780subject
Has Abstractpub_date
2012-11-01 00:00:00pages
984-6issue
11eissn
8755-1039issn
1097-0339journal_volume
40pub_type
杂志文章abstract::Cytohistologic correlation was performed by 3 observers on 100 atypical squamous cells of undetermined significance (ASCUS) cases from a colposcopy clinic. Our objectives were to: 1) subclassify ASCUS cases and determine their clinical significance; 2) assess the independent predictive value of different cytologic par...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199909)21:3<211::aid-dc14>
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Lobular endocervical glandular hyperplasia (LEGH) was first described by Nucci et al. in 1999 and is believed to be a precancerous lesion of minimal deviation adenocarcinoma and gastric-type adenocarcinoma in the uterine cervix. LEGH lesions do not always exhibit apparent cellular and structural atypia, so a...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24155
更新日期:2019-06-01 00:00:00
abstract::We compared the ThinPrep (TP) technique to the cytospin (CS) preparation in the cytological diagnosis of urine by processing 79 specimens by these two techniques. Ten cases were positive for malignancy (six high grade (HG)/carcinoma in situ; four low grade (LG) transitional cell carcinomas (TCC)). Forty-eight cases we...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10245
更新日期:2003-03-01 00:00:00
abstract::Non-Hodgkin lymphoma (NHL) is a heterogeneous group of lymphoid neoplasms and accurate subclassification is an essential prerequisite for proper management of patients. This study was aimed at evaluating the utility of nuclear morphometry and textural features on cytology smears to classify the cases of NHL on aspirat...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21154
更新日期:2010-02-01 00:00:00
abstract::The diagnostic significance of grooved nuclei in the cytology of extrathyroid lesions is not well-described in the literature. Isolated cases of metastatic adult granulosa cell tumor, reactive mesothelial hyperplasia, Langerhans' cell histiocytosis, recurrent renal cell carcinoma, and round cell liposarcoma were revie...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199701)16:1<57::aid-dc14>3
更新日期:1997-01-01 00:00:00
abstract::Epithelioid hemangioendothelioma (EHE) of the liver is an extremely rare tumor that masquerades as an epithelial neoplasm and poses significant diagnostic pitfalls for the cytopathologist. This report describes a 64-year-old woman whom on computerized tomography was found to have multiple peripherally calcified hypode...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.22943
更新日期:2014-06-01 00:00:00
abstract::Hepatic angiomyolipoma (HAML) is a rare mesenchymal neoplasm that belongs to the perivascular epithelioid tumor family. Though it is characteristically, a triphasic tumor composed of smooth muscle, blood vessels, and adipocytes, the smooth muscle cells are often epithelioid and can represent the near-entirety of the t...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24688
更新日期:2020-12-29 00:00:00
abstract::A chest wall abscess is a very rare presentation of extranodal Hodgkin's lymphoma (HL); only one case has been reported to date. Here, we describe a case of a 38-yr-old man with HL whose initial presentation was a chest wall abscess. The diagnosis of HL was suggested by cytological examination of the purulent discharg...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20220
更新日期:2005-07-01 00:00:00
abstract::We present a case in which a primary diagnosis of gout was made by fine-needle aspiration. The patient had a right distal ulnar mass, and the initial clinical and radiological diagnoses were that of giant cell tumor of tendon sheath. While tophi are the hallmark of gout, they rarely undergo aspiration because of the k...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0339(199609)15:3<246::AID-DC14>
更新日期:1996-09-01 00:00:00
abstract::The objective of this study was to analyze large-scale genomic patterns during disease progression from primary tumor to effusion in ovarian carcinoma, and to study the association between DNA ploidy parameters in effusions, proliferation/survival markers, and clinicopathologic characteristics. DNA ploidy status, DNA ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20879
更新日期:2008-09-01 00:00:00
abstract::Sequential tracheal aspirates from 39 neonates with hyaline membrane disease were examined to correlate cytological findings with the development of bronchopulmonary dysplasia (BPD). A total of 224 tracheal aspirates were examined from these infants, 15 of whom developed BPD as diagnosed by conventional clinical and r...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840020307
更新日期:1986-09-01 00:00:00
abstract::Preoperative chemotherapy for locally advanced breast carcinoma (stage IIIA,B) is increasingly utilized demonstrating a 70 to 95% objective response and 15 to 35% complete response. A 70-yr-old woman presented with a 9 cm left upper outer quadrant breast mass associated with overlying skin redness and a 2.5 cm left ax...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840100413
更新日期:1994-01-01 00:00:00
abstract::Lipoblastoma is an uncommon lipomatous tumor that typically occurs in infants and children. It may present as a single subcutaneous nodule or with multiple lesions (lipoblastomatosis). We describe fine-needle aspiration (FNA) cytologic features of two cases that presented as a subcutaneous lump in the scapular area an...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.20073
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Current clinical practices are shifting towards utilizing less invasive biopsy techniques, including fine needle aspiration (FNA) and needle core biopsies. If a patient has a suspected hematologic malignancy, a portion of the FNA sample is typically submitted for flow cytometry (FC) analysis, providing valua...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24417
更新日期:2020-06-01 00:00:00
abstract::Fine-needle aspiration biopsy findings in three cases of chromophobe cell carcinoma are described and correlated with histologic and ultrastructural observations. In addition, comparisons are made with three cases each of oncocytoma and granular cell carcinoma. The cells in aspiration smears from chromophobe cell carc...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840130403
更新日期:1995-11-01 00:00:00
abstract::Solitary fibrous tumor is a rare lesion of serosal membranes. In a pleural location, it may be confused clinically with a localized mesothelioma or a peripheral lung tumor. A transthoracic needle aspiration can be done to obtain material for a diagnosis in these easily accessible tumors. In the literature, there are o...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199802)18:2<118::aid-dc7>3
更新日期:1998-02-01 00:00:00
abstract::We evaluated the diagnostic contribution of adjunct studies performed on aspirated material in the work-up of pediatric fine-needle aspiration (FNA) biopsies. Ancillary studies were performed on 54 of 136 (39.7%) pediatric FNA biopsies during a 5-year period. In 23 (16.9%) cases, immunocytochemical (ICC) studies, cons...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840080210
更新日期:1992-01-01 00:00:00
abstract::The cytologic diagnosis of papillary thyroid carcinoma is straightforward in most instances. However, there are some mimics including goitrous nodules and Hurthle cell neoplasms. Many studies have shown the combination of HBME-1 and CK19 expression to be useful in reaching a correct histologic diagnosis on tissue sect...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20841
更新日期:2008-08-01 00:00:00
abstract::Endometrical cytology sampling using the Endo-pap sampler is an inexpensive, painless, and simple procedure. Tissue microfragments obtained include surface epithelial sheets, gland segments, and stromal cell aggregates. Gland segments are critical for evaluation of the sample. Glands are dilated in hyperplasia and the...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840010104
更新日期:1985-01-01 00:00:00
abstract::Since cyclophosphamide and busulphan used for therapy of bone marrow transplantation (BMT) can cause urothelial cell changes similar to those found in bladder cancer, comparative morphologic and morphometric urinary cytologic research was carried out, examining 812 urine samples taken from 121 patients undergoing BMT ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840080306
更新日期:1992-01-01 00:00:00
abstract::The simplicity and safety of the fine-needle aspiration (FNA) make it a useful procedure for the initial evaluation of supraclavicular lymphadenopathy. We analyzed data (106 patients) in combination with the data of three recently published studies (total 301 patients) to make the observations more meaningful. The use...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.10002
更新日期:2001-12-01 00:00:00
abstract::Necrosis is a worrisome cytologic feature in breast aspirates which, when paired with suspicious clinical and mammographic findings, could potentially result in a false positive diagnosis. A 66-yr-old female presented with clinical evidence of stage 1 breast carcinoma supported by the mammographic and ultrasound findi...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840110216
更新日期:1994-01-01 00:00:00
abstract::We report a rare case of extranodal NK/T-cell lymphoma, nasal type of the uterine cervix that showed cytologic features mimicking cervical cancer. A 65-year-old woman presented with vaginal bleeding. Gynecological examination revealed a bulky tumor of the cervix. A conventional Papanicolaou-stained cervical smear show...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23439
更新日期:2016-05-01 00:00:00
abstract::Serous tubal intraepithelial carcinoma (STIC) has attracted attention as a precursor lesion of high-grade serous carcinoma (HGSC) of the ovary. We report the rare case of a woman in whom adenocarcinoma cells were detected in cervical smears and demonstrated to be derived from STIC in the fimbria. The patient was a 48-...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23957
更新日期:2018-11-01 00:00:00
abstract::Although pleural and pericardial effusions are common and may rarely be the initial manifestation of disease, there are few reports of SLE diagnosed in a cytopathology laboratory. We describe an unusual case of SLE in a 75-year-old man who presented with cardiac tamponade. Cytologic examination of the pericardial flui...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199805)18:5<346::aid-dc8>3
更新日期:1998-05-01 00:00:00
abstract::Anaplastic large-cell lymphoma (ALCL) is a rare T-cell lymphoma typically seen in children and young adults. It has been described in numerous sites; however, the breast is one of the least common locations. We herein report a case of ALCL arising in the breast of a 36-yr-old pregnant woman. To our knowledge this is t...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20442
更新日期:2006-04-01 00:00:00
abstract::A 47-yr-old man with history of metastatic low-grade rectal leiomyosarcoma presented with progressive protrusion of his left eye due to an enlarging orbital mass. The differential diagnosis included tumor metastasis or orbital infection due to an unknown infectious agent. Diagnostic fine-needle aspiration (FNA) of the...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199711)17:5<369::aid-dc10>
更新日期:1997-11-01 00:00:00
abstract::Osteoblastomas are rare benign but sometimes aggressive primary bone tumors (1%), usually seen in males in the 2nd decade. Osteoblastomas, occurring in the spine, constitute a medical emergency due to impending cord compression. Hence a rapid and simple diagnostic modality, such as fine-needle aspiration (FNA) plays a...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23175
更新日期:2015-03-01 00:00:00
abstract::Clear cell hepatocellular carcinoma (CCHCC) is an uncommon morphologic variant of HCC and rarely invades into the main bile ducts. Here we describe a case of CCHCC that was diagnosed by bile duct brushing cytology. Liquid-based preparation of brushing specimen showed clusters of atypical epithelial cells with abundant...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23397
更新日期:2016-02-01 00:00:00
abstract::Neuroglial heterotopias (NGH) are rare congenital head and neck lesions composed of differentiated neuroectodermal tissue and representing developmental heterotopias rather than true neoplasms. The case of a male neonate with respiratory distress and early feeding problems depicting a retropharyngeal space mass which ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21575
更新日期:2011-11-01 00:00:00