Abstract：
:Understanding the relationship between physical exercise, reactive oxygen species and skeletal muscle modification is important in order to better identify the benefits or the damages that appropriate or inappropriate exercise can induce. Unbalanced ROS levels can lead to oxidation of cellular macromolecules and a major class of protein oxidative modification is carbonylation. The aim of this investigation was to study muscle protein expression and carbonylation patterns in trained and untrained animal models. We analyzed two muscles characterized by different metabolisms: tibialis anterior and soleus. Whilst tibialis anterior is mostly composed of fast-twitch fibers, the soleus muscle is mostly composed of slow-twitch fibers. By a proteomic approach we identified 15 protein spots whose expression is influenced by training. Among them in tibialis anterior we observed a down-regulation of several glycolitic enzymes. Concerning carbonylation, we observed the existence of a high basal level of protein carbonylation. Although this level shows some variation among individual animals, several proteins (mostly involved in energy metabolism, muscle contraction, and stress response) appear carbonylated in all animals and in both types of skeletal muscle. Moreover we identified 13 spots whose carbonylation increases after training.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Magherini F,Abruzzo PM,Puglia M,Bini L,Gamberi T,Esposito F,Veicsteinas A,Marini M,Fiorillo C,Gulisano M,Modesti Adoi
10.1016/j.jprot.2011.10.017subject
Has Abstractpub_date
2012-01-04 00:00:00pages
978-92issue
3eissn
1874-3919issn
1876-7737pii
S1874-3919(11)00526-4journal_volume
75pub_type
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