Abstract：
:Metabolite concentrations in gastrocnemius and soleus muscles were compared in young-adult (4 months), mature (12 months) and senescent (24 months) rats after continuous (72 consecutive hours) exposure to normobaric hypoxia or normoxia with the intraperitoneal administration of the vasodilator naftidrofuryl or saline solution for 30 days consecutively before hypoxia. The following metabolites were assessed in gastrocnemius muscle in relation to: (a) energy mediators: ATP, ADP, AMP; (b) energy store: creatine phosphate; (c) anaerobic glycolysis: glycogen, glucose, glucose 6-phosphate, pyruvate, lactate; (d) Krebs' cycle: citrate, alpha-ketoglutarate, malate; (e) free amino acids related to Krebs' cycle: aspartate, glutamate, alanine; and (f) ammonia. In the soleus muscle only ATP, creatine phosphate, glycogen, glucose, glucose 6-phosphate, pyruvate, lactate, citrate, alpha-ketoglutarate malate, aspartate and glutamate were assessed. Aging does not seem to affect soleus and gastrocnemius muscles in the same way. Some gastrocnemius muscle metabolites show linear changes in their concentrations with aging, while for the soleus muscle the only linear change relates to glucose 6-phosphate. As regards the influence of hypoxia on muscular metabolism, all the most important changes observed in metabolite concentrations in comparison with control values take place at the age of 4 and 24 months. Furthermore, as regards naftidrofuryl action, the most important variations observed concern only 4-month-old animals. Finally our data show that only in certain cases has pharmacological treatment been able to modify the influence of hypoxic conditions on the concentration of muscle metabolites, regardless of the age of the animals.
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
Pastoris O,Dossena M,Allorio F,Vercesi Ldoi
10.1016/0047-6374(90)90051-gsubject
Has Abstractpub_date
1990-06-01 00:00:00pages
207-19issue
3eissn
0047-6374issn
1872-6216pii
0047-6374(90)90051-Gjournal_volume
54pub_type
杂志文章abstract：:During experiments on the molecular basis of morphological and functional changes observed in rat liver mitochondria upon aging, we found that the buoyant density profile of mitochondrial DNA (mtDNA) shows a wide distribution pattern especially in the lighter region than that of young rat liver mtDNA. The heterogeneou...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(91)90040-7
更新日期：1991-11-01 00:00:00
abstract：:T cells in vivo have been shown to accumulate DNA damage with age. To investigate the effects of DNA damage on T cell biology we have utilised an in vitro human CD4+ T cell clone model. Levels and types of DNA damage were determined in 11 independent T cell clones as a function of their in vitro lifespan. Increased le...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(01)00254-8
更新日期：2001-08-01 00:00:00
abstract：:In the present work, we investigated whether IGF-1 regulates the transcription of the genes encoding the L-type Ca2+ channel (DHPR) channel and RyR1 in young adult and senescent mice. To this end, a transgenic mouse model overexpressing IGF-1 exclusively in skeletal muscle (S1S2) was studied at different ages and the ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(00)00236-0
更新日期：2001-04-15 00:00:00
abstract：:The age-related changes in the distribution of enkephalin-immunoreactive (ENK-IR) nerve fibers in relation to the principal neurons were studied in 23 human sympathetic ganglia from patients aged between 22 and 98 years. There were no age-related changes in the diameter of nerve cells and the packing density of the ne...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(88)90086-3
更新日期：1988-08-01 00:00:00
abstract：:To investigate the relationship between immunological potential and advance of age, patterns of humoral immune response in different aged rabbits were compared. In the primary response, the appearance of total antibody and the arrival to its peak level were retarded by advance of age. On the other hand, the appearance...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(76)90038-5
更新日期：1976-01-01 00:00:00
abstract：:In the present study, a number of physiological and behavioral measures that are related to metabolism were continuously monitored in 19-month-old male Fischer 344 rats that were fed ad libitum or fed a caloric restricted diet. Caloric restricted rats ate fewer meals but consumed more food during each meal and spent m...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/0047-6374(89)90044-4
更新日期：1989-05-01 00:00:00
abstract：:Detailed structural, mutational, and biochemical analyses of human FEN1/DNA complexes have revealed the mechanism for recognition of 5' flaps formed during lagging strand replication and DNA repair. FEN1 processes 5' flaps through a previously unknown, but structurally elegant double-stranded (ds) recognition/single s...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/j.mad.2011.11.009
更新日期：2012-04-01 00:00:00
abstract：:The strychnine-insensitive binding of [3H]glycine was characterized in purified cerebral cortical membranes from mice aged from 7 days to 22 months. The binding was saturable, exhibiting only one component during the whole life-span studied. The binding constant KD did not change during development and ageing, whereas...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(93)90131-a
更新日期：1993-11-01 00:00:00
abstract：:In an attempt to determine the relevance of the free radical theory of ageing to age changes discernible in connective tissue parameters, a small colony of C3H/He and LAF1 mice was set up, with sample culled at intervals throughout the lifespan to provide experimental samples. To half of the stock a dietary supplement...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(80)90004-4
更新日期：1980-11-01 00:00:00
abstract：:The data concerning the effects of age on the brainstem are scarce and few works are devoted to the human vestibular nuclear complex. The study of the effects of aging in the vestibular nuclei could have clinical interest due to the high prevalence of balance control and gait problems in the elderly. We have used in t...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(00)00098-1
更新日期：2000-04-14 00:00:00
abstract：:The impairment of brown adipose tissue (BAT) thermogenic activity with aging has been well documented. The current study investigated the effect of swimming training on BAT activity in 2-month-old (young) and 26-month-old (old) male mice. The trained mice underwent a 6-week swimming program (1 h/day, 5 days/week) in w...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(96)01727-7
更新日期：1996-08-15 00:00:00
abstract：:There are reports demonstrating elevated levels of autoantibodies in elderly people. We now analyzed whether the strong inflammatory response associated with aging is interrelated with the production of autoantibodies, antinuclear antibodies (ANA). In a cohort of 284 nonagenarians the rate of ANA positivity was 12.3%,...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2007.03.001
更新日期：2007-05-01 00:00:00
abstract：:During senescence, autofluorescent lysosomal storage bodies known as lipofusin or age pigment accumulate in many post-mitotic types of cells. Among these cell types is the retinal pigment epithelium (RPE) of the mammalian eye. The mechanisms of lipofuscin formation and accumulation have been studied more extensively i...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2004.11.004
更新日期：2005-04-01 00:00:00
abstract：:Previous studies on DNA repair in ageing have demonstrated increased frequencies of single and double strand breaks in lymphocytes from elderly subjects and, as a consequence, decreased efficiency in DNA replication. We have investigated the relationship between cell proliferation and the nuclear expression of ku prot...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(97)00137-1
更新日期：1998-01-30 00:00:00
abstract：:Accumulation of oxidative DNA damage in the human brain has been implicated in etiologies of post-traumatic and age-associated declines in neuronal function. In neurons, because of high metabolic rates and prolonged life span, exposure to free radicals is intense and risk for accumulation of damaged DNA is amplified. ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/j.mad.2008.02.001
更新日期：2008-07-01 00:00:00
abstract：:Some observations on cellular senescence are discussed regarding the possibility that the postulated genes, which bring about the DNA synthesis block of senescent fibroblasts, might be similar to those designated as anti-oncogenes or tumour suppressor genes. The latter genes have been defined by tumorogenicity tests o...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/0047-6374(90)90044-g
更新日期：1990-04-30 00:00:00
abstract：:The presence of damaged and microbial DNA can pose a threat to the survival of organisms. Cells express various sensors that recognize specific aspects of such potentially dangerous DNA. Recognition of damaged or microbial DNA by sensors induces cellular processes that are important for DNA repair and inflammation. He...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/j.mad.2016.09.001
更新日期：2017-07-01 00:00:00
abstract：:The ascidian Ciona intestinalis has a short life span and powerful regeneration capacities. The regeneration of the oral siphon (OS) involves wound healing, blastema formation, cell proliferation, and replacement of 8 oral pigment organs (OPO), the latter via differentiation and migration of stem/precursor cells from ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2012.08.003
更新日期：2012-09-01 00:00:00
abstract：:The effects of interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), and lipopolysaccharide (LPS) on the productions of nitric oxide (NO) and cGMP by cultured articular chondrocyte (AC) monolayers from 1-, 8- and 18-month old male Wistar rats were studied. It was found that basal NO and cGMP productions decre...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(97)00150-4
更新日期：1998-03-16 00:00:00
abstract：:As the endothelium ages it may become more susceptible to damage by atherogenic plasma components such as toxic lipid oxidation products. Vitamin E (vit E) might prove to be anti-atherogenic by reducing oxidative injury. This study investigated the effects of age and chronic exposure to fatty acid hydroperoxides (OFA)...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(90)90110-2
更新日期：1990-10-01 00:00:00
abstract：:Previous studies have suggested a possible relationship between the ubiquitin-proteasome pathway and some pathological manifestations of Alzheimer's disease (AD). This study investigated the possibility that the Abeta peptides interact with the ubiquitin-proteasome pathway inside neuronal cells. The ubiquitin-proteaso...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2005.07.006
更新日期：2005-12-01 00:00:00
abstract：:Concanavalin A (Con A)-mediated red blood cell (RBC) adsorption with the RBC coating method (in which Con A-coated RBC's are adsorbed to fibroblasts) was greatly increased by glutaraldehyde fixation of RBCs before Con A-coating and decreased by the fixation of fibroblasts. On the other hand, RBC adsorption with the fi...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(79)90003-4
更新日期：1979-11-01 00:00:00
abstract：:The steroid hormone ecdysone influences Drosophila lifespan. Longevity is extended in mutants deficient for ecdysone synthesis or mutants of the ecdysone receptor (EcR). However, the underlying mechanisms remain unclear. Here we conditionally inactivated EcR by RNA interference or expression of dominant negative forms...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2009.05.004
更新日期：2009-08-01 00:00:00
abstract：:Circulating antibodies in the sera of normal, healthy humans of different age groups to native DNA (nDNA) and reactive oxygen species modified DNA (ROS-DNA) was studied by competition ELISA. Sera from the young population (< 50 years of age) showed negligible levels of anti-DNA antibodies. In contrast, anti-DNA antibo...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(98)00025-6
更新日期：1998-06-01 00:00:00
abstract：:The dynamics of in vitro uptake of [14C]-ascorbic acid into intact lenses of young (50 days old) and old (730 days old) guinea pigs was evaluated in this study. Two-dimensional protein gel analysis of [35S]-methionine labeled proteins provided evidence that the isolated lenses were viable throughout the culture period...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(90)90025-b
更新日期：1990-08-01 00:00:00
abstract：:We showed earlier that life spans of murine B lymphocytes could be estimated by measuring the functional reactivities of normal B cells upon transfer into x-linked immunodeficient (xid) mice, which do not respond to anti-mouse IgM (anti-mu) antibodies and thymic-independent type-2 (TI-2) antigens. Here the same approa...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(91)90012-o
更新日期：1991-12-02 00:00:00
abstract：:This paper is the second one in a series of two papers hypothesizing and testing systemic grounds of reproductive life history in the female fruit fly. In the first paper, we analyzed mechanisms of individual fecundity scheduling and have drawn the following conclusions. Individual fecundity in female flies is endowed...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2003.10.007
更新日期：2004-01-01 00:00:00
abstract：:The intertwining between epilepsy, sleep disorders and beta amyloid pathology has been progressively highlighted, as early identification and stratification of patients at high risk of cognitive decline is the need of the hour. Modification of the sleep-wake activity, such as sleep impairment or excessive daytime slee...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/j.mad.2021.111434
更新日期：2021-01-11 00:00:00
abstract：:Activity of (Na+,K+)ATP-ase measured by means of influx of 86Rb was compared in lymphocytes from young, middle-age and old people. It was found that the (sensitive to ouabain) activity of (Na+,K+)ATPase was significantly diminished in the lymphocytes from aged subjects. ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(85)90105-8
更新日期：1985-12-01 00:00:00
abstract：:A quantitative ultrastructural study of the posterior pituitary was undertaken. The posterior pituitary is the storage of the hormones vasopressin and oxytocin. Female C57BL/Icrfat mice which were under conditions of normal hydration were sampled at 8 and 32 months of age. There was a slight age-related decline in the...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(81)90069-5
更新日期：1981-02-01 00:00:00
