Abstract:
:Potassium chromate induced the formation of DNA-protein complexes in cultured Chinese hamster ovary cells. The DNA-protein complexes were isolated by ultracentrifugal sedimentation in the presence of 2% sodium dodecyl sulfate (SDS) and 5 M urea. Two-dimensional SDS-polyacrylamide gel electrophoresis analysis of the chromate-induced DNA-protein complexes revealed that two acidic proteins of 53 and 45 kDa and a basic protein of 54 kDa were selectively complexed to the DNA. Numerous other proteins also became associated with the DNA to a lesser degree as the chromate concentration was increased. Nuclease digestion was not a prerequisite for the resolution of the protein component of the DNA-protein complexes using two-dimensional gel electrophoresis. Ultracentrifugal analysis of the DNA-protein complexes in the presence of proteinase K, nucleases, or a chelating agent demonstrated that protein aggregation was not responsible for the increased protein recovery in chromate-treated samples and that the complexes were disrupted by EDTA. These data suggest that the selectively complexed proteins were associated with the DNA through strong interactions that may be mediated by the trivalent form of chromium.
journal_name
Mol Carcinogjournal_title
Molecular carcinogenesisauthors
Miller CA 3rd,Costa Mdoi
10.1002/mc.2940010208subject
Has Abstractpub_date
1988-01-01 00:00:00pages
125-33issue
2eissn
0899-1987issn
1098-2744journal_volume
1pub_type
杂志文章abstract::Preventive measures against oral carcinogenesis are urgently warranted to lower the high morbidity and mortality associated with this malignancy worldwide. Here, we investigated the chemopreventive efficacy of grape seed extract (GSE) and resveratrol (Res) in 4-nitroquinoline-1-oxide (4NQO)-induced tongue tumorigenesi...
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更新日期:2016-04-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:Molecular carcinogenesis
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更新日期:2013-06-01 00:00:00
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更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2017-04-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940140411
更新日期:1995-12-01 00:00:00
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更新日期:2009-11-01 00:00:00
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更新日期:2017-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.21874
更新日期:2013-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.20233
更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/(sici)1098-2744(199805)22:1<46::aid-mc6>3.
更新日期:1998-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.22458
更新日期:2016-12-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2744(199612)17:4<175::AID-MC1>3
更新日期:1996-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.1058
更新日期:2001-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.22023
更新日期:2014-09-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章,评审
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更新日期:2012-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/(sici)1098-2744(199902)24:2<128::aid-mc7>3
更新日期:1999-02-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940050207
更新日期:1992-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940050112
更新日期:1992-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22021
更新日期:2014-09-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20836
更新日期:2012-08-01 00:00:00