Abstract:
:Mutations in the Ki-ras oncogene and the p53 tumor suppressor gene are known to occur at high frequencies in human colon cancers. We measured the frequency of mutations in these two genes in colon adenocarcinomas obtained from a widely used experimental model of human colon carcinogenesis: F344 rats treated with the carcinogens azoxymethane (AOM) or dimethylhydrazine (DMH). We detected codon 12 mutations in Ki-ras in approximately 60% of colon adenocarcinomas induced by either carcinogen. We characterized the rat p53 intron-exon junctions to construct primers for polymerase chain reaction amplification of this gene. We discovered that the rat p53 gene was structurally different from the human p53 gene, as the rat gene was missing one intron between exons 6 and 7. Both single-stranded DNA conformational polymorphism analysis and direct DNA sequencing of the highly conserved regions of rat exons 5-7 were conducted because the corresponding human regions (exons 5-8) have been reported as being mutated most frequently in human colon cancers. Using these methods, we were unable to identify any p53 mutations in the highly conserved regions of exons 5-7 in either AOM- or DMH-induced colon adenocarcinomas. These data confirm that Ki-ras was mutated in most colon cancers in AOM- or DMH-treated rats but indicate that molecular alterations in the p53 gene, if they occur in this animal model, are different from most p53 mutations in human colon cancers.
journal_name
Mol Carcinogjournal_title
Molecular carcinogenesisauthors
Erdman SH,Wu HD,Hixson LJ,Ahnen DJ,Gerner EWdoi
10.1002/(sici)1098-2744(199707)19:2<137::aid-mc8>3subject
Has Abstractpub_date
1997-06-01 00:00:00pages
137-44issue
2eissn
0899-1987issn
1098-2744pii
10.1002/(SICI)1098-2744(199707)19:2<137::AID-MC8>3journal_volume
19pub_type
杂志文章abstract::Previous studies have established the in vivo bioavailability and efficacious dosages of phenylbutyl isoselenocyanate (ISC-4), a selenium-substituted isothiocyanate, against mouse xenograft models of human melanoma and colorectal cancer. To explore its potential attributes against prostate cancer, we treated human LNC...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22825
更新日期:2018-08-01 00:00:00
abstract::Cancer cells mainly rely on glycolysis for energetic needs, and mitochondrial ATP production is almost inactive. However, cancer cells require the integrity of mitochondrial functions for their survival, such as the maintenance of the internal membrane potential gradient (DeltaPsim). It thus may be predicted that Delt...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20539
更新日期:2009-08-01 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is becoming one of the major causes of hepatocellular carcinoma (HCC) in the United States and Western countries; however, the molecular mechanisms associated with NASH-related liver carcinogenesis are not well understood. In the present study, we investigated cancer-associated chr...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22818
更新日期:2018-08-01 00:00:00
abstract::Centchroman (CC), a female oral contraceptive, has been shown to possess breast anti-cancer activities. Recently, we have shown CC-mediated antimetastatic effect through reversal of epithelial-to-mesenchymal transition (EMT) in breast cancer. The loss of tumor suppressor genes (TSGs) has been shown to promote EMT in b...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22424
更新日期:2016-11-01 00:00:00
abstract::Increased epidermal growth factor receptor (EGFR) expression in triple-negative breast cancer (TNBC) is recognized as a promising therapeutic target, specifically through the use of selective EGFR inhibitors combined with chemotherapies. TNBC is characterized by genetic instability that leads to increased sensitivity ...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22596
更新日期:2017-05-01 00:00:00
abstract::Triptolide is an active component from a Chinese herb, Tripterygium wilfordii which has been applied for treating immune-related diseases over centuries. Recently, it was reported that a variety of cancer cell lines could be sensitized to DNA-damage based chemotherapy drugs in combination with Triptolide treatment. In...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22795
更新日期:2018-06-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that is frequently over-expressed in human cancers and is associated with tumorigenesis, and increased tumor proliferation and progression. Also found in breast tumors with high levels is B-Myb, a transcription factor whose expression is activated b...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20147
更新日期:2006-01-01 00:00:00
abstract::Polymorphisms in metabolic and DNA repair genes may alter protein function, consequently affecting patients' response to chemo/radiotherapy. We retrospectively assessed whether polymorphisms of glutathione-S-transferase genes GSTM1 (deletion), GSTT1 (deletion), GSTP1 (Ile105Val, rs1695), and DNA repair genes hOGG1 (Se...
journal_title:Molecular carcinogenesis
pub_type: 临床试验,杂志文章
doi:10.1002/mc.21868
更新日期:2012-10-01 00:00:00
abstract::Sex-determining region Y box (SOXs) are expressed in various cells and control cell fate and differentiation in a multitude of physiologic processes. SOX6, a main representative of SOXs, is involved in the regulation of carcinogenesis in various human malignancies. However, the role of SOX6 in clear cell renal cell ca...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.23246
更新日期:2020-10-01 00:00:00
abstract::Human colorectal cancer (CRC) cells are resistant to the anti-proliferative effect of transforming growth factor-β (TGF-β), suggesting that disruption of TGF-β signaling plays an important role in colorectal carcinogenesis. Ecotropic virus integration site-1 (Evi-1) oncoprotein represses TGF-β signaling by interacting...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.21852
更新日期:2013-04-01 00:00:00
abstract::Flavonoids are the most investigated phytochemicals due to their pharmacological and therapeutic activities. Their ability to chelate with metal ions has resulted in the emergence of a new category of molecules with a broader spectrum of pharmacological activities. In this study, the ruthenium quercetin complex has be...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22792
更新日期:2018-06-01 00:00:00
abstract::Indole-3-carbinol (I3C), an anti-cancer phytochemical derived from cruciferous vegetables, strongly inhibited proliferation and down-regulated protein levels of the melanocyte master regulator micropthalmia-associated transcription factor (MITF-M) in oncogenic BRAF-V600E expressing melanoma cells in culture as well as...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22472
更新日期:2017-01-01 00:00:00
abstract::The E5 open reading frame (ORF) from bovine papillomavirus type 1 (BPV 1) as well as the E5 ORFs from human papillomaviruses (HPV) type 6 and type 16 have been reported to transform immortalized rodent cells. In an analysis of murine and human tumors for the presence of putative papillomavirus-related sequences, we cl...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940060204
更新日期:1992-01-01 00:00:00
abstract::It is well known that the TP53 gene considerably influences on DNA repair processes. Polymorphisms in the TP53 gene, particularly the well-known Arg72Pro in codon 72 of exon 4 (Ex4+119 G>C; rs1042522), can modify the functionality of the p53 protein and activation of DNA repair. Actually, polymorphic variants Arg and ...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20633
更新日期:2010-06-01 00:00:00
abstract::In this report we characterized the transcriptional regulation of the rat mdr1b gene by xenobiotics. The expression of this gene was increased in primary rat hepatocytes and in the H4-II-E hepatoma cell line by exposure to carcinogens such as aflatoxin B1, N-acetoxy-2-acetylaminofluorene, and methyl methanesulfonate. ...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940130109
更新日期:1995-05-01 00:00:00
abstract::In this report, we describe in detail the evolving procedures to optimize humanized mouse cohort generation, including optimal conditioning, choice of lineage for engraftment, threshold for successful engraftment, HNSCC tumor implantation, and immune and stroma cell analyses. We developed a dual infusion protocol of h...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22887
更新日期:2018-11-01 00:00:00
abstract::The tumor suppressor p27, which is a member of the Cip/Kip family of Cyclin-dependent kinase inhibitory proteins (CKIs), controls anti-proliferative events. The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22490
更新日期:2017-01-01 00:00:00
abstract::Notch signaling plays an important role in ovarian cancer chemoresistance, which is responsible for recurrence. Gamma-secretase inhibitor (GSI) is a broad-spectrum Notch inhibitor, but it has serious side effects. The efficacy of Notch3-specific inhibition in paclitaxel-resistant ovarian cancers was assessed in this s...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22363
更新日期:2016-07-01 00:00:00
abstract::The atomic bombing of Hiroshima and Nagasaki and the nuclear accident at Chernobyl raised the question of prenatal sensitivity to ionizing radiation-induced cancer. In this study, mice were exposed to single doses of gamma-radiation (0.2-2.0 Gy) at different embryonic stages. The tumor incidence increased with dose fr...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Promoter hypermethylation is gaining strength as one of the main mechanisms through which tumor suppressor genes are silenced during tumor progression. Three tumor suppressor genes are frequently found methylated in their promoter, in concordance with absence of expression, RASSF1A, SLIT2, and WIF1. In addition, a pre...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.21881
更新日期:2013-06-01 00:00:00
abstract::Studies with murine models suggest that maternal exposure to aromatic hydrocarbon receptor (AhR) agonists may impair mammary gland differentiation and increase the susceptibility to mammary carcinogenesis in offspring. However, the molecular mechanisms responsible for these perturbations remain largely unknown. Previo...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22095
更新日期:2015-04-01 00:00:00
abstract::Regions of genomic DNA containing repetitive nucleotide sequences can adopt a number of different structures in addition to the canonical B-DNA form: many of these non-B DNA structures are causative factors in genetic instability and human disease. Although chromosomal DNA replication through such repetitive sequences...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章,评审
doi:10.1002/mc.20508
更新日期:2009-04-01 00:00:00
abstract::RhoBTB2 was isolated recently as a tumor suppressor gene from human chromosome 8p21.3. Although RhoBTB2 was found to be frequently lost in breast cancer lines, expression status of RhoBTB2 in sporadic breast cancer tissues and its clinical and prognostic value, however, remain unclear. Tissue samples from breast cance...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20598
更新日期:2010-03-01 00:00:00
abstract::Rapid advances in multimodality therapy have not significantly improved the overall 5-yr survival of oral cancer patients in the past two decades, thereby underscoring the need for molecular therapeutics. The development of new treatment strategies for more effective management of oral cancer requires identification o...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20048
更新日期:2005-02-01 00:00:00
abstract::This study examines the relationship between p53 immunostaining and direct sequencing of polymerase chain reaction (PCR) products in 61 gliomas. Glioma tissues obtained from patients at surgery were analyzed immunohistochemically with the monoclonal antibody PAb1801 to detect p53 protein abnormalities. Amplified p53 c...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2744(199601)15:1<1::AID-MC1>3.0
更新日期:1996-01-01 00:00:00
abstract::Thymidylate synthase (TYMS) is involved in the folate metabolism and provision of nucleotides needed for DNA synthesis and repair. Thus, functional genetic variants in TYMS may alter cancer risk. In the study, we evaluated associations of three germline variants (rs2790 A > G, rs16430 6 bp > 0 bp, and rs1059394 C > T)...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22097
更新日期:2015-04-01 00:00:00
abstract::The majority of ovarian cancers over-express the estrogen receptor (ERα) and grow in response to estrogens. We previously demonstrated that ER induction of the chemokine CXCL12 (stromal cell-derived factor-1) is required for estradiol (E2)-stimulated proliferation of human ovarian carcinoma cells. In the current study...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.21913
更新日期:2013-09-01 00:00:00
abstract::Ezrin is known to regulate cellular survival, adhesion, migration, and invasion and has been identified as one of the key components of tumor progression and metastasis. The authors investigated ezrin expression in human hepatocellular carcinoma (HCC) and sought to determine its relation with clinicopathologic paramet...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20653
更新日期:2010-09-01 00:00:00
abstract::Colorectal cancer (CRC) is one of the most common malignant tumors worldwide. As tumor metastasis is the leading cause of death in patients with CRC, it is important to elucidate the molecular mechanisms that drive CRC metastasis. Studies have shown a close relationship between Iroquois homeobox (IRX) family genes and...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.23098
更新日期:2019-11-01 00:00:00
abstract::Obesity and its associated metabolic syndrome (MetS) are recognized risk factors for breast cancer. The molecular basis for this association remains largely unknown. Adipokines, in particular leptin and adiponectin, are thought to form part of the mechanism linking obesity with cancer through their altered expression/...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20764
更新日期:2011-08-01 00:00:00