Abstract:
:Human pluripotent stem cells (hPSCs) offer a multifaceted platform to study cardiac developmental biology, understand disease mechanisms, and develop novel therapies. Remarkable progress over the last two decades has led to methods to obtain highly pure hPSC-derived cardiomyocytes (hPSC-CMs) with reasonable ease and scalability. Nevertheless, a major bottleneck for the translational application of hPSC-CMs is their immature phenotype, resembling that of early fetal cardiomyocytes. Overall, bona fide maturation of hPSC-CMs represents one of the most significant goals facing the field today. Developmental biology studies have been pivotal in understanding the mechanisms to differentiate hPSC-CMs. Similarly, evaluation of developmental cues such as electrical and mechanical activities or neurohormonal and metabolic stimulations revealed the importance of these pathways in cardiomyocyte physiological maturation. Those signals cooperate and dictate the size and the performance of the developing heart. Likewise, this orchestra of stimuli is important in promoting hPSC-CM maturation, as demonstrated by current in vitro maturation approaches. Different shades of adult-like phenotype are achieved by prolonging the time in culture, electromechanical stimulation, patterned substrates, microRNA manipulation, neurohormonal or metabolic stimulation, and generation of human-engineered heart tissue (hEHT). However, mirroring this extremely dynamic environment is challenging, and reproducibility and scalability of these approaches represent the major obstacles for an efficient production of mature hPSC-CMs. For this reason, understanding the pattern behind the mechanisms elicited during the late gestational and early postnatal stages not only will provide new insights into postnatal development but also potentially offer new scalable and efficient approaches to mature hPSC-CMs.
journal_name
Pediatr Cardioljournal_title
Pediatric cardiologyauthors
Marchianò S,Bertero A,Murry CEdoi
10.1007/s00246-019-02165-5subject
Has Abstractpub_date
2019-10-01 00:00:00pages
1367-1387issue
7eissn
0172-0643issn
1432-1971pii
10.1007/s00246-019-02165-5journal_volume
40pub_type
杂志文章,评审abstract::We present a 2-month-old male infant with thrombosis in the superior vena cava and pericardium due to transient protein C deficiency. Protein C deficiency was related to sepsis and hepatitis-induced liver function impairment. The patient's cardiac anatomy was otherwise normal. The patient was referred to us with signs...
journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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更新日期:2005-07-01 00:00:00
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
pub_type: 杂志文章
doi:10.1007/BF00796330
更新日期:1994-05-01 00:00:00
abstract::We sought to characterize the shifting epidemiology and resource utilization of Lyme disease and associated carditis in US children's hospitals. We hypothesized that the Lyme carditis burden has increased and that hospitalizations for Lyme carditis are costlier than those for Lyme disease without carditis. The PHIS da...
journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
pub_type: 杂志文章
doi:10.1007/BF02505087
更新日期:1996-03-01 00:00:00
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journal_title:Pediatric cardiology
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更新日期:2014-10-01 00:00:00
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journal_title:Pediatric cardiology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Pediatric cardiology
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doi:10.1007/s00246-018-1857-0
更新日期:2018-06-01 00:00:00
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journal_title:Pediatric cardiology
pub_type: 杂志文章
doi:10.1007/s00246-019-02087-2
更新日期:2019-06-01 00:00:00
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journal_title:Pediatric cardiology
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更新日期:2005-09-01 00:00:00
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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journal_title:Pediatric cardiology
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