Abstract：
OBJECTIVE:Metformin is one of the most widely used drugs for the treatment of type 2 diabetes. Recent investigations demonstrated that application of metformin reduces cancer risk. The present study aimed to determine the role of liver kinase B1 (LKB1) in the response of cervical cancer cells to metformin. METHODS:LKB1 expression and the integrity of LKB1-AMPK signaling were determined with immunoblot in 6 cervical cancer cell lines. Cellular sensitivity to metformin was analyzed with MTT assay. RESULTS:Metformin inhibited growth of cervical cancer cells, C33A, Me180, and CaSki, but was less effective against HeLa, HT-3, and MS751 cells. Analyzing the expression status and the integrity of LKB1-AMPK-mTOR signaling, we found that cervical cancer cells sensitive to metformin were LKB1 intact and exerted an integral AMPK-mTOR signaling response after the treatment. Ectopic expression of LKB1 with stable transduction system or inducible expression construct in endogenous LKB1 deficient cells improved the activation of AMPK, promoted the inhibition of mTOR, and prompted the sensitivity of cells to metformin. In contrast, knock-down of LKB1 compromised cellular response to metformin. Our further investigation demonstrated that metformin could induce both apoptosis and autophagy in cervical cancer cells when LKB1 is expressed. CONCLUSIONS:Metformin is a potential drug for the treatment of cervical cancers, in particular to those with intact LKB1 expression. Administration of cell metabolism agonists may enhance LKB1 tumor suppression, inhibit cell growth, and reduce tumor cell viability via the activation of LKB1-AMPK signaling.
journal_name
Gynecol Oncoljournal_title
Gynecologic oncologyauthors
Xiao X,He Q,Lu C,Werle KD,Zhao RX,Chen J,Davis BC,Cui R,Liang J,Xu ZXdoi
10.1016/j.ygyno.2012.06.032subject
Has Abstractpub_date
2012-10-01 00:00:00pages
249-55issue
1eissn
0090-8258issn
1095-6859pii
S0090-8258(12)00483-0journal_volume
127pub_type
杂志文章abstract：OBJECTIVE:To test if TP53 hot spot mutations (HSMs) confer differential chemotherapy resistance or survival outcomes, the effects of microtubule stabilizers on human ovarian carcinoma cells (OCCs) expressing TP53 HSMs were studied in vitro. Survival outcomes of patients with high grade serous epithelial ovarian carcino...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2015.04.039
更新日期：2015-07-01 00:00:00
abstract：:Clear cell carcinoma of the ovary accounts for 2 to 3% of all epithelial ovarian neoplasms. Patient profiles, pathological characteristics, and results of treatment are reviewed for 59 patients. The median age was 51. Disease extent at diagnosis was as follows: stage I, 18 patients (31%); stage II, 20 patients (34%); ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(89)90043-7
更新日期：1989-11-01 00:00:00
abstract：:The steroid receptor content of the primary endometrial cancer of 22 patients who were treated for recurrent or advanced disease has been measured and correlated with response to medroxyprogesterone acetate. No patient with a progesterone receptor (PR)-negative tumor responded and only 2 patients with PR-positive tumo...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(85)90269-0
更新日期：1985-07-01 00:00:00
abstract：:Two patients with vulvar carcinoma are reported who were treated with internal iliac transcatheter arterial embolization and radiotherapy. Patient 1 had T3N3M0 vulvar carcinoma. The lesion remaining after radiotherapy was treated by bilateral internal iliac arterial embolization. Vulvectomy was then performed, and the...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(90)90407-c
更新日期：1990-10-01 00:00:00
abstract：OBJECTIVES:Based on our previous report showing that orobol, a potent phosphatidylinositol 4-kinase (PI4K) inhibitor, produced cisplatin (DDP) sensitivity, we have determined the mechanism of orobol-sensitization effect. METHODS AND RESULTS:Orobol produced >2-fold DDP sensitivity in human ovarian carcinoma 2008 cells ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/s0090-8258(03)00316-0
更新日期：2003-08-01 00:00:00
abstract：BACKGROUND:Angiomyolipoma is a distinctive benign neoplasm of the renal origin. Rare extrarenal instances of angiomyolipoma have been documented at various body sites. A case of extrarenal angiomyolipoma arising from the parametrial soft tissues is described in the present report. CASE:A woman, age 40 years, presented...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/s0090-8258(03)00251-8
更新日期：2003-07-01 00:00:00
abstract：OBJECTIVES:To evaluate reasons for discontinuing intraperitoneal (IP) chemotherapy, and to compare characteristics of patients who did versus did not successfully complete six cycles of IP chemotherapy. METHODS:In a phase III trial, women with optimal stage III ovarian or peritoneal carcinoma were randomly allocated t...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.ygyno.2005.11.013
更新日期：2006-01-01 00:00:00
abstract：OBJECTIVE:The aim of this trial was to investigate the efficacy and toxicity of a relative high-dose of topotecan combined with carboplatin in recurrent or persistent epithelial ovarian cancer (EOC). METHODS:Patients participating in this phase II trial received topotecan at a dose of 1.0 mg/m(2)/day intravenously (IV...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2009.04.016
更新日期：2009-08-01 00:00:00
abstract：OBJECTIVE:To help determine whether global collaborations for prospective gynecologic surgery trials should include hospitals in developing countries, we compared surgical and oncologic outcomes of patients undergoing laparoscopic radical hysterectomy at a large comprehensive cancer center in the United States and a ca...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.01.007
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to evaluate the impact of the 1999 national recommendations for ovarian cancer surgery in England to be performed by specialist surgeons in specialist centres. METHODS:A retrospective analysis of English cancer registry records, Hospital Episode Statistics (HES) data for all English ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2015.03.003
更新日期：2015-09-01 00:00:00
abstract：:Lymph node sampling is part of the FIGO staging of patients with ovarian carcinoma and is usually part of a meticulous second look operation. We analyzed the primary lymph node status of patients and compared this to the lymph node status at second look operation. From 3/86-3/91, 97 patients with epithelial ovarian tu...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(92)90097-3
更新日期：1992-11-01 00:00:00
abstract：BACKGROUND:The presence of cardiac metastasis from cervical carcinoma is extremely rare. The diagnosis is made almost exclusively postmortem. There are few cases of premortem diagnosis, and it is believed that when cardiac metastasis are found in vivo, the prognosis is extremely poor. Due to the rarity of this conditio...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1006/gyno.1998.5009
更新日期：1998-06-01 00:00:00
abstract：OBJECTIVES:Limited knowledge exists about the value of tumor size in surgically treated cervical cancer (CX) using a tumor size of 2 cm as cut-off value. METHODS:A total of 366 cases of CX FIGO stage IB who received upfront surgery were evaluated regarding tumor size, the prediction of pelvic lymph node involvement, a...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2014.04.011
更新日期：2014-07-01 00:00:00
abstract：:An estimated 2-5% of endometrial cancers and 15-20% of high-grade, non-mucinous epithelial ovarian cancers have an underlying hereditary cause. Appropriate risk assessment, genetic counseling, and germline genetic testing for cancer predisposition genes in both gynecologic cancer patients and their at-risk relatives i...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2019.01.024
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVE:To determine if extra-peritoneal laparoscopic para-aortic (PA) lymphadenectomy allows a reliable assessment of PA nodes in patients with endometrial cancer (EC). METHODS:In October of 2005, a single surgeon began performing extra-peritoneal laparoscopic PA lymphadenectomy for patients with EC. A prospective ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2008.08.021
更新日期：2008-12-01 00:00:00
abstract：BACKGROUND:Traditionally, hysterectomy is considered definitive therapy for cervical adenocarcinoma in situ (AIS) in women beyond childbearing. CASE:A 45-year-old gravida 2, para 2 patient presented with cervical dysplasia and on pathology review of the large loop excision procedure cervical adenocarcinoma in situ was...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.06.044
更新日期：2005-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the feasibility of the sentinel lymph node (SLN) identification with SPECT/CT lymphoscintigraphy imaging in the early stage invasive cervical cancer in patients undergoing radical hysterectomy and pelvic lymphadenectomy. METHODS:Between March 2007 and June 2009, a pro...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2010.12.336
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to determine whether para-aortic lymphadenectomy improves disease-related survival (DRS) in stage IIIc endometrial cancer. METHODS:A total of 63 patients with stage IIIc endometrial carcinoma underwent primary radical surgery in the Tohoku Gynecologic Cancer Unit from 1993 to 20...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2007.06.009
更新日期：2007-11-01 00:00:00
abstract：OBJECTIVES:In this study, we analyzed the PTEN expression in a large collection of clear cell adenocarcinomas of the ovary. Furthermore, we analyzed the expression of cyclin D1 and p27, and investigated the correlation among all these variables. METHODS:Totally, 40 clear cell adenocarcinomas were included in this stud...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.09.047
更新日期：2006-04-01 00:00:00
abstract：OBJECTIVE:To evaluate the utility of amiodarone and its derivative dronedarone as novel drug repositioning candidates in EOC and to determine the potential pathways targeted by these drugs. METHODS:Drug-predict bioinformatics platform was used to assess the utility of amiodarone as a novel drug-repurposing candidate i...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2018.09.019
更新日期：2018-12-01 00:00:00
abstract：:For the past several years, all women with epithelial ovarian cancer have been treated identically, whether in a clinical trial or off protocol. Over the past decade, we have come to appreciate the magnitude of the heterogeneity of ovarian cancer. The development of the binary grading system for serous carcinoma was a...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2013.05.021
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the clinicopathological features of ovarian Sertoli-Leydig cell tumors (SLCTs) and to explore the reasonable therapy. METHODS:A total of 40 cases of SLCTs were retrospectively reviewed. RESULTS:The incidence of SLCTs was 0.41%, with a median age of 28 years. All tumors were confined to one ovary...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.07.114
更新日期：2012-11-01 00:00:00
abstract：:Effective cytotoxic treatment options for advanced cervical cancer are exceedingly limited. Cisplatin-based combination chemotherapy, the most commonly used cytotoxic therapy, has produced response rates ranging from 20% to 30% and overall survival of less than 10 months. Because of the minimal degree of success with ...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2008.04.016
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:A LEEP-Cone may not be necessary for all patients with traditional cone indications. This study defines populations where a single pass technique with the LEEP is appropriate. METHODS:We retrospectively reviewed patients undergoing LEEP-Cone procedures performed at the University of Oklahoma Health Science C...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.09.015
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to examine the relationships between cyclooxygenase-2 (COX-2) expression and prognostic factors in cervical carcinomas. METHODS:We studied COX-2 expression in 53 women with cervical cancers, including 35 squamous cell carcinomas (SCCs), 1 adenosquamous cell carcinoma (ASCC), and...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/s0090-8258(02)00066-5
更新日期：2003-03-01 00:00:00
abstract：OBJECTIVE:Despite the fact that endometrial cancer commonly occurs in elderly women, little is known about the outcome of the oldest old, those > 80 years of age. We examined the patterns of care and outcome of the oldest old women with endometrial cancer. METHODS:An analysis of women > 65 years of age with endometrio...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2011.02.040
更新日期：2011-07-01 00:00:00
abstract：:From 1971 through 1981, 81 women received 4350 to 5075 rad to the periaortic lymph nodes as part of their primary management for carcinoma of the uterine cervix. While two patients developed chronic small bowel damage, only one required surgical intervention. Five-year disease-free survival was 40%. Approximately one-...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(83)90110-5
更新日期：1983-02-01 00:00:00
abstract：OBJECTIVE:The purpose of this case-controlled study was to determine the prevalence of anemia and incidence of perioperative blood transfusions in patients undergoing treatment for advanced ovarian cancer with neoadjuvant chemotherapy (NACT) or primary debulking surgery (PDS). METHODS:We performed a single institution...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2018.05.014
更新日期：2018-07-01 00:00:00
abstract：:Toxic shock syndrome is a multisystem disease which presents with a high fever, rash, gastrointestinal symptoms, and hypotension. A 58-year-old woman presented to the emergency room with these symptoms, hypotension and tachycardia. Because of vaginal spotting a pelvic examination was performed which demonstrated vulvo...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1994.1043
更新日期：1994-02-01 00:00:00
abstract：OBJECTIVES:Lack of exercise and excess body weight may exacerbate treatment-related declines in quality of life (QoL) in endometrial cancer survivors. The primary purpose of this study was to examine the associations among exercise, body weight, and QoL in a population-based sample of endometrial cancer survivors. MET...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.01.007
更新日期：2005-05-01 00:00:00
