Abstract:
BACKGROUND:Pediatric glioblastoma is a malignant disease with an extremely poor clinical outcome. Patients usually suffer from resistance to radiation therapy, so targeted drug treatment may be a new possibility for glioblastoma therapy. Survivin is also overexpressed in glioblastoma. YM155, a novel small-molecule survivin inhibitor, has not been examined for its use in glioblastoma therapy. METHODS:The human glioblastoma cell line M059K, which expresses normal DNA-dependent protein kinase (DNA-PK) activity and is radiation-resistant, and M059J, which is deficient in DNA-PK activity and radiation-sensitive, were used in the study. Cell viability, DNA fragmentation, and the expression of survivin and securin following YM155 treatment were examined using MTT (methylthiazolyldiphenyl-tetrazolium) assay, ELISA assay, and Western blot analysis, respectively. RESULTS:YM155 caused a concentration-dependent cytotoxic effect, inhibiting the cell viability of both M059K and M059J cells by 70% after 48 hours of treatment with 50 nM YM155. The half-maximal inhibitory concentration (IC50) was around 30-35 nM for both cell lines. Apoptosis was determined to have occurred in both cell lines because immunoreactive signals from the DNA fragments in the cytoplasm were increased 24 hours after treatment with 30 nM YM155. The expression of survivin and securin in the M059K cells was greater than that measured in the M059J cells. Treatment with 30 nM YM155, for both 24 and 48 hours, significantly suppressed the expression of survivin and securin in both cell lines. CONCLUSION:The novel survivin inhibitor YM155 elicits potent cytotoxicity in glioblastoma cells in vitro via DNA-PK-independent mechanisms. YM155 could be used as a new therapeutic agent for the treatment of human glioblastomas.
journal_name
Pediatr Neonatoljournal_title
Pediatrics and neonatologyauthors
Lai PC,Chen SH,Yang SH,Cheng CC,Chiu TH,Huang YTdoi
10.1016/j.pedneo.2012.04.008subject
Has Abstractpub_date
2012-06-01 00:00:00pages
199-204issue
3eissn
1875-9572issn
2212-1692pii
S1875-9572(12)00058-7journal_volume
53pub_type
杂志文章abstract::Nystagmus is an involuntary rhythmic oscillation of the eyes, which leads to reduced visual acuity due to the excessive motion of images on the retina. Nystagmus can be grouped into infantile nystagmus (IN), which usually appears in the first 3-6 months of life, and acquired nystagmus (AN), which appears later. IN can...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章,评审
doi:10.1016/j.pedneo.2014.02.007
更新日期:2014-10-01 00:00:00
abstract::Glycogen storage disease (GSD) type Ib is caused by the deficiency of glucose-6-phosphate translocase activity. The elder brother of the proband died at age 20 months, and GSD Ia, a disease caused by the deficiency of glucose-6-phosphatase, was the diagnosis. Theproband developed hypoglycemia shortly after birth. Diet...
journal_title:Pediatrics and neonatology
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更新日期:2009-06-01 00:00:00
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journal_title:Pediatrics and neonatology
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更新日期:2015-02-01 00:00:00
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journal_title:Pediatrics and neonatology
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更新日期:2016-08-01 00:00:00
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journal_title:Pediatrics and neonatology
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
abstract::Recently, heated humidified high-flow nasal cannula (HHHFNC) has been introduced and applied as a noninvasive respiratory support in neonates. Although HHHFNC is widely used in neonates presenting with respiratory distress, the efficiency and safety when compared with nasal continuous positive airway pressure or nonin...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章,评审
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abstract:BACKGROUND:The parameters of oxidative stress [advanced oxidation protein products (AOPPs), malondialdehyde (MDA), and S100B] and the effect of intensive phototherapy (PT) on these parameters have not been studied extensively in newborns with significant hyperbilirubinemia (SH). We aimed to measure the levels of MDA, S...
journal_title:Pediatrics and neonatology
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journal_title:Pediatrics and neonatology
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更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:To investigate the clinical values of serum melatonin and αII spectrin cleavage products (SBDPs) in assessing the severity of brain injury in preterm infants. METHODS:Sixty-four premature infants in total were selected and classified into the brain injury group (BI, n = 30) and the non-brain injury group (C...
journal_title:Pediatrics and neonatology
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abstract::Head trauma is one of the most challenging fields of traumatology and demands immediate attention and intervention by first-line clinicians. Symptoms can vary from victim to victim and according to the victim's age, leading to difficulties in making timely and accurate decisions at the point of care. In children, fall...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章,评审
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abstract:BACKGROUND:Excessive noise in nurseries has been found to cause adverse effects in infants, especially preterm infants in neonatal intensive care units (NICUs). The NICU design may influence the background sound level. We compared the sound level in two differently designed spaces in one NICU. We hypothesized that the ...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/S1875-9572(09)60076-0
更新日期:2009-12-01 00:00:00
abstract::Nonocclusive mesenteric ischemia (NOMI) is induced by intestinal vasospasm without thromboembolic occlusion and is associated with high morbidity and mortality. The estimated overall incidence of autopsy-verified fatal NOMI is 2.0 cases/100,000 person-years; however, no pediatric or adolescent cases have yet been repo...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2014.07.008
更新日期:2017-02-01 00:00:00
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journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2016.09.010
更新日期:2017-12-01 00:00:00
abstract::Pediatric cardiomyopathies are a group of myocardial diseases with complex taxonomies. Cardiomyopathy can occur in children at any age, and it is a common cause of heart failure and heart transplantation in children. The incidence of pediatric cardiomyopathy is increasing with time. They may be associated with variabl...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章,评审
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更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:This study identified factors associated with the recurrence of appendicitis in children with appendiceal masses after successful nonsurgical treatment. METHODS:In this retrospective study, children who were diagnosed as having appendiceal masses after undergoing conservative treatment between 2000 and 2014...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2016.12.001
更新日期:2017-08-01 00:00:00
abstract::Respiratory distress syndrome (RDS) was recognized to be caused by primary surfactant deficiency almost 70 years ago and continuous positive airway pressure was introduced approximately 50 years ago. Since then, there have been many developments in neonatology; we know many things but others are still controversial. T...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2020.11.005
更新日期:2020-12-05 00:00:00
abstract:BACKGROUND:The survival of children with malignant germ cell tumor (GCT) increased over the past 2 decades with platinum-based chemotherapy. This report has three objectives: (1) comparison of PVB (cisplatin, vinblastine, and bleomycin) with JEB (carboplatin, etoposide, and bleomycin) regimens; (2) treatment modality o...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2014.12.003
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Parents often express concerns about feeding difficulties in their child. We hypothesized that these parental concerns were associated with adverse growth status in early childhood. We aimed to determine the prevalence of such concerns and whether these concerns were associated with adverse growth status in ...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2019.04.004
更新日期:2019-12-01 00:00:00
abstract::Internal herniation is a rare cause of intestinal obstruction, especially in the emergency department. We report a child with acute abdomen resulting from transmesenteric internal herniation of the small bowel. Radiographic findings revealed gaseous distension of the bowel loops in the upper abdominal area with a pauc...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2012.12.008
更新日期:2014-04-01 00:00:00
abstract::The prevalence of atopic dermatitis (AD) appears to have increased dramatically over the past decades. It is generally believed that such rapid increase in prevalence cannot be explained fully by genetic factors. Environmental factors might play a role in such an increment. Children with AD are most likely to suffer c...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章,评审
doi:10.1016/j.pedneo.2015.08.005
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abstract:BACKGROUND:Airway remodeling refers to the structural changes in the airways of asthma. Caveolin-1 reduces cell growth and negatively regulates smooth muscle cell proliferation. The aim was to investigate lung caveolin-1 status in a murine model of acute allergic airway disease. METHODS:Six- to eight-week-old female B...
journal_title:Pediatrics and neonatology
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doi:10.1016/j.pedneo.2010.12.006
更新日期:2011-02-01 00:00:00
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journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2015.03.015
更新日期:2016-02-01 00:00:00
abstract::Multicystic dysplastic kidney (MCDK) is a congenital maldevelopment in which the renal cortex is characteristically replaced by numerous cysts of multiple sizes. MCDK presenting as a single predominant large cyst in morphology is less common. We report on the prenatal imaging findings and perinatal management of a fet...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2011.05.009
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:Community-acquired Gram-Negative (GN) bacteremia caused more morbidity and mortality recently in children. The increasing drug resistance was also an important issue. However, published reference was few about children. METHODS:We conducted a retrospective study to collect febrile patients with blood cultur...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00
abstract:BACKGROUND:Over the years the need for surgical treatment, timing of intervention, and the type of surgical approach have been discussed, but the treatment of congenital lung malformations remains controversial. The aim of this study was to compare the thoracotomy approach with the thoracoscopic technique by evaluating...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2015.10.009
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:The majority of children born with very low birth weight (VLBW; < 1500g) enter mainstream schools. They experience significant neurodevelopmental disabilities during childhood. The specific aims of our study were to evaluate the neonatal outcomes of VLBW infants and whether they would influence intelligence ...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/S1875-9572(08)60005-4
更新日期:2008-04-01 00:00:00
abstract:AIM:We aimed to define the etiologic and prognostic factors in live-born infants with hydrops fetalis (HF) in our tertiary neonatal intensive care unit over a 10-year period. METHODS:Medical records of newborn infants with HF during 2002-2011 were reviewed retrospectively. Demographic data, prenatal interventions, cli...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2013.07.008
更新日期:2014-04-01 00:00:00
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journal_title:Pediatrics and neonatology
pub_type: 杂志文章,随机对照试验
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更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:The goal of this study was to compare the growth and effect of growth on cognitive performance at 5 years of age of a group of very-low-birth-weight (VLBW) infants and a group of healthy full-term infants. METHODS:Beginning in 1995, under the sponsorship of the Premature Baby Foundation, the Society of Neon...
journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2013.08.001
更新日期:2014-04-01 00:00:00
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journal_title:Pediatrics and neonatology
pub_type: 杂志文章
doi:10.1016/j.pedneo.2017.02.003
更新日期:2018-02-01 00:00:00