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Applicability of 2-DE to assess differences in the protein profile between cold storage and not cold storage in nectarine fruits.

Abstract：

:Cold storage is being used to increase nectarine fruits' postharvest life. However, low temperatures lead to chilling injury and limit their commercial quality and value. In this study a proteomic approach was used to compare the protein profile between control and cold storage nectarine fruits. Protein extracted from tissue was resolved by two-dimensional gel electrophoresis (2-DE) in the 4-7 pH and 10-200 kDa Mr range. Around 350 spots were well determined, and 11 from 17 spots that showed significant differences were identified by peptide mass fingerprinting (PMF) using matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS). Four differentially expressed proteins were characterized as allergens and were further assessed at the transcription level using quantitative real time-RT-PCR (qRT-PCR).

journal_name

J Proteomics

journal_title

Journal of proteomics

authors

Giraldo E,Díaz A,Corral JM,García A

doi

10.1016/j.jprot.2012.08.005

subject

Has Abstract

pub_date

2012-10-22 00:00:00

pages

5774-82

issue

18

eissn

1874-3919

issn

1876-7737

pii

S1874-3919(12)00598-2

journal_volume

75

pub_type

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